MULTILAYERS AND POLY（ALLYLAMINE HYDROCHLORIDE）-GRAFT- POLY（ETHYLENE GLYCOL） MODIFIED BOVINE SERUM ALBUMIN NANOPARTICLES： IMPROVED STABILITY AND pH-RESPONSIVE DRUG DELIVERY

To improve the colloidal stability of bovine serum albumin （BSA） nanoparticles （hiPs） in diverse mediums, poly（allylamine hydrochloride） （PAH）/sodium poly（4-styrene sulfonate） （PSS） multilayers and poly（allylamine hydrochloride）-graft-poly（ethylene glycol） （PAH-g-PEG） coating were coated on the surface of BSA NPs. Stabilities of the BSA NPs in diverse mediums with different surfaces were detected by dynamic light scattering （DLS）. Multilayers and PAH-g-PEG coated BSA NPs can be well dispersed in various mediums with a narrow polydispersity index （PDI）. The BSA NPs with the highest surface density of PEG show the best stability. The multilayers and PAH-g-PEG coating do not deter the pH-dependent loading and release property of BSA NPs. At pH 9, the encapsulation efficiency of doxorubicin reaches almost 99%, and the release rate at pH 5.5 is significantly higher than that at pH 7.4.