A Comparative Study of the Expressions of ets-2, IGF-Ⅱ,C-myc and N-ras in Human Primary Hepatocellular Carcinoma and Tumor-adjacent Tissues

The expressions of ets-2 ,IGF-Ⅱ,C-myc and N-ras in 12 pairs ofhuman primary hepatocellular carcinoma(PHC)and tumor-adjacent tissues are presentedin this paper.The results showed that there was at least one of the four oncogenesstudied over-expressed in the 12 pairs of samples.Ets-2 was the most commonly ex-pressed oncogene seen in all the PHC and tumor-adjacent tissues,with 3.5 and 2.4 Kb asthe major two bands,which are different from the evenly expressed 4.5 ,3.5 and 2.4 Kbbands in the normal control livers.In 6 tumor-adjacent tissues,the expression ofets-2 was higher than that in PHC.IGF-Ⅱ was expressed as 5.0 and 2.0 Kb fetaltranscripts in PHC and tumor-adjacent tissues,while in the normal control livers thetranscript was 5.6 Kb only.In one tumor-adjacent tissue there were IGF-Ⅱ fetal tran-scripts ,but in the corresponding PHC no IGF-Ⅱ transcripts were detected .N-raswas expressed as 4.0 kb band in 8 out of 12 PHC and in 6 out of 12 tumor-adjacent tis-sues.In two cases the expression of N-ras was higher in tumor-adjacent tissues than inPBC.5.6 and 2.6 Kb N-ras transcripts were also detected in one pair of PHC and tumor-adjacent tissues and in two tumor-adjacent tisues only,together with the 4.0 Kbtranscript.C-myc was expressed as 4.0 Kb band in 9 out of 12 PHC and in 6 out of12 tumor-adjacent tissues.One tumor-adjacent tissue had higher C-myc expression thanPHC In two PHC ,a 2.2 Kb C-myc transcript was also detected.The roles and rela-tionships of these oncogenes in the carcinogenesis of human PHC are discussed.