Effect of Zibu Shenjing Fang（滋补肾精方） on Growth and Development of the Mouse with Kidney-essence Insufficiency and Study on the Mechanism

Objective：To investigate effects of Zibu Shenjing Fang（滋补肾精方） on growth and development of the mouse with insufficiency of kidney-essence and the mechanism.Methods：Total 50 mice were randomly divided into a normal group,a model group,a Jingui Shenqi Wan（金匮肾气丸） group,a Zibu Shenjing Fang high dose group and a Zibu Shenjing Fang low dose group,10 mice in each group.The kidney-essence insufficiency mouse model was established by use of threat-injuring the kidney combined with over-fatigue.At the same time of modeling,the mice in the model group were intragastrically administrated with saline 20 mL·kg-1·d-1,in the Jingui Shenqi Wan group with suspension of the Jingui Shenqi Wan 2.7 g·kg-1·d-1,in the Zibu Shenjing Fang high dose group with Zibu Shenjing Fang 20 g·kg-1·d-1 and in the Zibu Shenjing Fang low dose group with Zibu Shenjing Fang 10 g·kg-1·d-1,for 21 consecutive days.The general state was observed,the body weight was weighted,and serum growth hormone（GH） and insulin-like growth factor-1（IGF-1） contents were detected.Results：Compared with model group,Zibu Shenjing Fang groups and Jingui Shenqi Wan group could improve manifestation of the mouse with kidney-essence insufficiency,increase body weight of the mouse and serum GH and IGF-1 contents,especially in the high dose group.Conclusion：Zibu Shenjing Fang gives play to the function of tonifying the kidney and replenishing essence through regulating GH and IGF-1 levels,so as to influence growth and development of the mouse.