STUDY ON PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES TO Anabaena azollae

Forty hybridoma cell lines secreting monoclonal antibodies against Anabaena azollae have been established by fusing SP2/O myeloma cells with spleen cells from BALB/c mice immunized with purified antigen Anabaena azollae from different strains of azolla.According to the fluorescent antibody test, 40 McAbs were divided into 8 types. They may differentiate the Anabaena azollae in 195 azolla strains which are collected from different places in the world, into 8 antigenic groups.The identifying result of Anabaena azollae isolated from reconstituted azolla and sexual hybrid azolla showed that the Anabaena azollae in the reconstituted azolla retains its antigenicity from the donor azolla, and Anabacna azollae in hybrid azolla maintains its antigenicity from the female parent plant.