Effect of 2’-O-methylation on the activity of ribozyme

The catalytic structure of a hammerhead ribozyme is composed of 3 helixes and10-nucleotide residue single strand regions (numbered 3—9. 12—14). In our previous pa-pers, three double strand regions were called stems. and now they are called helixes uponthe suggestion of Hertel et al.The previous stem Ⅰ is now called helix Ⅲ, stem Ⅲ iscalled helix Ⅰ,and stem Ⅱ is called helix Ⅱ. The study on the catalytic mechanism ofribozyme in our laboratory indicated that the base pairing strength and property in helixes