STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS

The isolated laminin receptor （LN-R） labeled by 125I was reconstituted into liposomes.125I-LN-R-liposomes and free 125I-LN-R were separated by Sepharose 4B column chromatogra-phy. The LN-R-liposomes showed affinity for laminin （LN） and were capable of binding toimmobilized LN substrate. In order to make transplantation of LN-R, LN-R-liposomes werefused with cultured murine Lewis lung carcinoma cells with the help of polyethylene glycol（PEG） induction. The radiation with the fused cells was not removed by salt sclution. Thebinding of the fused cells enriched in foreign LN-R to LN substrate increased by 87.5%.Furthermore, the murine Lewis lung carcinoma cells with and without transplanted LN-Rwere injected into C57BL/6J mice through tail veins （5×105 cells/each mouse）respectively.The mice in the test group died earlier than those in the control group. The total weight oflung tumor in the test group remarkably increased in comparison with those in the controlgroup. The results taken together