CRYSTALLOGRAPHIC STUDY ON HIGHLY STABLE HUMAN INSULINS——CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF A21-SER MUTANT

CRYSTALLOGRAPHIC STUDY ON HIGHLY STABLE HUMAN INSULINS——CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF A21-SER MUTANT

It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes place readily due to the deamidation of this residue. As a number of pharmaceutical p...

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Veröffentlicht in:中国科学通报：英文版 1992 (16), p.1390-1393
1. Verfasser: 胡永林 伍伯牧 张英 王大成 N.C.KAARSHOLM R.NORRIS
Format: Artikel
Sprache:eng
Schlagworte:
A21-Ser
crystallization
human
insulin
mutant
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Zusammenfassung:It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes place readily due to the deamidation of this residue. As a number of pharmaceutical preparations of insulin are acidic solutions, this kind of instability has brought about a serious problem on purity and storage of this preparation in clinical
ISSN:2095-9273