A study of hepatic energy metabolism disturbance and its management in acute obstructive cholangitis in rats

The mitechondrial respiratory rate(S)of hepatocytes,respiratory control ratio(RCR),oxidative phosphorylation effidency(ADP/O),and hepatic ATP level were determined in ratswith acute obstructive cholangitis(AOC)to observe the changes of mitochondrial respiration,andthe therapeutic effects of verapamil and dexamethasone on the changes were assessedIt was found that S especially its state IV was significantly accelerated and RCR,ADP/O,and ATP level of the liver decreased at 6h after AOC,but in those rats treated with verapamilor dexamethasone,no significant changes of these parameters were observed in 48 h period afterAOC.These facts suggest that in the early stage of AOC,severe impairment of the mitochondrialrespiratory function and disturbance of energy metabolism could be present,but verapamil anddexamethasone could effectively prevent these damages from occurring.