THE COEXPRESSION OF THE preS1（1—42） AND THE CORE （1—144） ANTIGEN OF HBV IN E.coli

Objective:To sutdy the therapeutic T cell vaccine for the treatment of chronic hepatitis B by improving the cellular immunization of HBsAg vaccine with the coexpression of the preS1(1-42)and the Core(1-144)antigen of HBV in E.coli.Methods:The genes of HBcAg（1-144) and preS1(1-42)were amplified and fused by PCR.This fused gene was inserted in the prokaryotic expression vector pET-11d and expressed in E.coli.Results:It was showed by SDS-PAGE that the protein moloecular weight of the coexpression product was about 20kD,20% of all bacteria protein.The monoclonal antibodies against core and preS1 atibody could react with this fused protein by Western-blot technique respectively.The fused gene was verified by sequencing.Under the immune electron microscopy,this fused protein is typical particles of HBcAg but in an aggregated form.Conclusion:The results might aid for studying T cell immunotherapeutic vaccine for chronic hepatitis B.