Preliminary Comparative Study on the Effect of Different Chinese Drugs for Strengthening Pi（脾）in Antagonizing Diet Induced Obesity

Objective：To observe the difference in fatty degree,glucose-lipid disorder and adipose-hormones between diet induced obesity（DIO） rats and diet induced obesity resistance（DIO-R） rats,and to explore the effect and acting mechanism of Chinese drugs for strengthening Pi（脾,CD-SP） and those for both strengthening Pi and dissolving phlegm（CD-SPDP） in inhibiting obesity.Methods：Excepting eight rats allocated in the blank control group,the other 54 rats were fed with high-lipid forage for 12 weeks to establish models of obesity. Finally,30 DIO rats and 8 DIO-R rats（shown by their body weight） were obtained.The DIO rats were divided into three groups,which were given gastric perfusion,respectively,with normal saline（Group A）,CD-SP（Group B）,and CD-SPDP（Group C）.Fourteen weeks later,the animals＇ body weight（BW）,length（BL）,blood levels of fasting insulin（Fln）,fasting glucose（FBG）,triglyceride（TG）,cholesterol（TC）,leptin（LP）,neuropeptide Y（NPY）, C-reactive protein（CRP）,tumor necrosis factor-α（TNF-α）,adiponectin（AN）,and resistin（RS） were measured; insulin resistance index（IRI） was calculated,and the degree of obesity and lipid content in abdominal cavity of rats were estimated.Moreover,the levels of LP,CRP,TNF-α,AN and RS in homogenate of rats＇ adipose tissues（ATH） were determined.Results：After 12 weeks of high-lipid diet,the BW of DIO rats was higher than that of normal or DIO-R rats.After a 14-week continuous high-lipid diet feeding,in DIO rats,BW,lipid coefficient （LC）,and IRI were significantly increased（P〈0.01）;serum levels of TNF-α,LP and AN were lower,NPY was higher,while the ATH levels of LP and AN were lower and TNF-αwas higher in DIO rats than in DIO-R rats （P〈0.05 or P〈0.01）;blood levels of FBG and lipids in DIO rats showed an increasing trend but was statistically insignificant（P〉0.05）;no significant difference was found in serum levels of CRP and RS due to the overly high data dispersion.Comparisons of the 3 DIO groups showed that BW,body weight index（BWI）,LC and IRI were significantly lowered after treatment（P〈0.01） in Group C,while these indexes were not significantly different between Group A and B;the serum levels of TNF-α,LP,and AN increased,NPY decreased in Group B and C, ATH levels of LP and AN increased,and TNF-αdecreased in the two groups;but NPY,LP,and AN in blood and ATH were higher in Group C than those in Group B（P〈0.05 or P〈0.01）.Conclusion：CD-SPDP could inhibit DIO and IR,showing that the effect is better than that of CD-SP,and its mechanism i