Streptavidin Capture and Detection Using Individual Agarose Bead-based Microfluidic Immunoassay Devices

A single microwell on polycarbonate substrate was fabricated using hot embossing by silicon master. The silicon master （85 μm in top, 100 μm in bottom , 53 μm in height） and 0.25 mm-thick polycarbonate substrate were sandwiched between two glass plates in hot embossing system. The system was heated to 155-160℃ and pressed with a force of 300 psi for 10-30 s. The single microwell was stampted on polycarbonate substrate. Apply a -0.2μL aliquot of agarose beads to the single microwell. After the solution nearly dried, individual beads were attached and embedded into the microwell using dry lint-free towelette. Excess beads were simultaneously removed from the microwell area.