Influence of Formate on Bioactivity Material-thuringiensin Synthesized by Baeillus thuringiensis YBT-032

The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellu...

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Veröffentlicht in:Journal of Wuhan University of Technology. Materials science edition 2008, Vol.23 (5), p.691-693
1. Verfasser: WANG Zhi CHEN Xiong CHEN Shouwen SUN Ming YU Ziniu
Format: Artikel
Sprache:eng
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Zusammenfassung:The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g^-1 · h and transformation ratio from glucose to thuringiensin increased by 43.30%.
ISSN:1000-2413
1993-0437