Helicobacterpylori Infection in Association with Cell Proliferation, Apoptosis and Prostaglandin E2 Levels

Objective： To evaluate the relationship between H. pylori infection with cell proliferation, apoptosis and PGE2 levels. Methods： A population-based study was conducted in Linqu, a high-risk area of gastric cancer in China. A total of 1523 subjects, aged 35-64, participating in a gastric cancer screening survey were investigated. H. pylori status were determined by 13C-urea breath test, expressions of Ki-67 were assessed by immunohistochemistry, apoptotic cells were detected by terminal deoxynucleotide transferase mediated dUTP nick end-labeling （TUNEL） method, and PGE2 levels were measured by enzyme immunoassay. Results： H. pylori infection was positively associated with cell proliferation activity. The mean and median percentage of Ki-67 labeling index （LI） in subjects with H. pylori positive were 14.1±10.3 and 12.0, significantly higher than those with H. pylori negative （ -↑x±s： 8.4±7.0; median： 5.8; P〈0.0001）. Moreover, the prevalence rates of H. pylori infection showed a tendency to increase according to severity score of cell apoptosis （Pmend 〈0.0001）, from score 0 to 3, the percentage of H. pylori positivity increased from 67.5% to 96.7%. Furthermore, The mean and median of PGE2 concentration were 628.84±726.40 pg/mL and 411.33 pg/mL among subjects with H. pylori positive compared with 658.19±575.91pg/mL and 455.97 pg/mL among those with H. pylori negative （P=0.209）. Conclusion： H. pylori infection was positively associated with increased cell proliferation and apoptosis activity, suggesting that H. pylori infection plays an important role in the gastric epithelial cell malignant transformation.