GenBank数据库中鳜鱼微卫星位点的筛选及特征分析

This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats （SSRs）, or microsatellites. A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites, account for 9.87% of wholly GenBank database. Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites, accounting for 53.33 % of the total microsatellite-containing sequences; 11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences, accounting for 36.66 % and 10.01% respective. 17 primer pairs were designed from these microsatellite sequences. Among the 17 primer pairs, 13 pairs have amplified products, 6 pairs can achieve clear PCR products by Electrophoresis. Genotype analysis results indicated that： the average polymorphism information contents （PIC） were 0.692.