Association of promoter polymorphism of the CD14 C （-159） T endotoxin receptor gene with chronic hepatitis B

AIM： To investigate whether single-nucleotide polymorphisms in the promoter regions of endotoxin-responsive genes CD14 C （-159） T is associated with chronic hepatitis B. METHODS： We obtained genomic DNA from 80 patients with established diagnosis of chronic hepatitis B and 126 healthy subjects served as a control population. The CD 14 C （-159） T polymorphism was investigated using an allele specific PCR method. RESULTS： Twenty seven percent of chronic hepatitis B patients and 75% of controls were heterozygous for CT genotype. The difference between the chronic hepatitis B and control groups was statistically significant [P 〈 0.0001; Odds ratio （OR） = 2.887; 95% CI： 1.609-5.178]. Twenty four point six percent of chronic hepatitis B and patients 12.3% of the control group were heterozygous for TT genotype. The difference between groups was not statistically significant （P = 0.256; OR = 0.658; 95% CI： 0.319-1.358）. Forty eight point four percent of chronic hepatitis B patients and 12.7% of control were homozygote for CC genotype （P 〈 0.004; OR = 0.416; 95% CI： 0,229-0,755）. The frequency of allele C was 61,9% and allele T was 38.1% in hepatitis B patients group. The frequency of allele C was 55.2% and allele T was 44.8% for the control group （P = 0.179; OR = 1.319; 95% CI： 0.881-1.977）. CONCLUSION： The TT heterozygous genotype was not a risk factor for chronic hepatitis B. CC homozygote genotype is protective for hepatitis B. Lack of heterozygosis of genotype CT is a risk factor for chronic hepatitis B. Alleles C or T were not risk factors for chronic hepatitis B. These findings show the role of a single-nucleotide polymorphism at CD14/-159 on the development of chronic hepatitis B. Endotoxin susceptibility may play a role in the pathogenesis of chronic hepatitis B.