Local Delivery of C-myc Antisense Oligodeoxynucleotide by Gelatin Coated Platinium-Iridium Stent to Prevent Restenosis in a Normal Rabbit Carotid Artery

Objectives To investigate the feasibility and effect of local deliveryof c-myc antisense oligodeoxynucleotide (ASODN) by gelatin coated Platinium-Iridium stent to prevent restenosis in a normal rabbit carotid artery. Methods Gelatin coated Platinium-Iridium stent were implanted in the right carotid arteries of 32 rabbits under vision. Animals were randomized to the control group and the treated group receiving c-myc ASODN (n=16 respectively).7,14,30,90days following the stenting procedure,morphometry for caculation of neointimal area and mean neointimal thickness were performed.The expression of c-mye protein was detected by immunohistochemical methods. Results 32 stents were successfully implanted into the right carotid arteries in 32 animals.Morphometrie analysis showed that neointimal area and mean neointimal thickness siginificantly increased continuously up to 12weeks after stent implantation,and at each time point,neointimal area and mean neointimal thickness were siginificantly smaller in the treated group than control group. (P