Electrochemical and Spectroscopic Studies on Interaction between Complex of Copper（Ⅱ） with Schiff Base Ligand and DNA

The crystal structure of ([CuL(H2O)2](ClO4)2·2H2O, CuL)(L=N- (5-sulfosalicylidene)-4'-bromoaniline) was determined by means of X-ray crystallography. The interaction between CuL and salmon Sperm DNA in a 0. 1 mol/L HOAc-NaOAc buffer solution (pH 5.10) was investigated by cyclic voltammetry and UV-Vis spectroscopy. A couple of redox peaks of CuL in the cyclic voltammograms(CV) can be seen at the glassy carbon electrode. It was found that the peak current of CuL decreased significantly with a negative shift of the peak potential in the presence of DNA compared with that in the absence of DNA, In addition,the decreased absorbance of CuL was observed with increasing the concentration of DNA; the absorption spectrum of an ethidium bromide(EB)-DNA system in the presence of CuL confirms that the binding mode of CuL to DNA is different from the intercalation of EB with DNA. All the experimental results indicate that CuL can bind to DNA by electrostatic binding and form a 1 : 1 association complex with a binding constant of 2. 72×10^4 L/mol.