Antioxidant Activity, Phenols, Flavonoids, Flavonols and Anthocyanins Content of Tussilago forfara (L.) Growing Wild in Kosovo

Total phenols, flavonoids, flavonols and anthocyanis content and antioxidant activity were quantitatively determined to the Tussilago fatfara (L.) growing wild in Sharr (South part of Kosova). In the tested extracts of plant species Tussilago fatfara (L.) quantitative composition of total phenols, flavonoids, flavonols and anthocyanins were determined by spectrophotometric method. Total phenols determined by Folin-Ciocalteu reagent and their amounts in the range of 56.50 mg/g to 84.70 mg/g (expressed as gallic acid equivalent, mg GAE/g). The amounts of flavonoids in plant extracts are in the range of 2.75 mg/g to 8.76 mg/g (expressed as rutin equivalent, mg RE/g).The amount of total flavonols in Tussilago farfara (L.) extracts ranged from 0.21 mg RE/g to 0.51 mg RE/g. The contents of total anthocyanosides in the Tussilago farfara (L) ranged from 1.44 (ethanol extract) to 1.86 (acetone extract) anthocyanins determined as mg yanidin 3-glucoside/g dry weight. Antioxidative activity was determined in vitro, using the DPPH reagent by spectrophotometric method.The results demonstrated that extract RSC was dependent on the solvent polarity, and the type of free radical used in the reaction. The IC50 values extracts in DPPH• assay was from 0.10 mg/ml (ethanol extract) to 0.20 mg/ml (acetone extract).