328 Ccr5δ32 (rs333) polymorphism is associated with susceptibility to systemic lupus erythematosus in female brazilian patients

Background and aimsThe role of CCR5Δ32(rs333) polymorphism in the pathogenesis of systemic lupus erythematosus (SLE) has been evaluated worldwide. The aim of this study was to determine the association between CCR5Δ32polymorphism with the susceptibility to SLE and the activity of disease in female Southern Brazilian patients.MethodsThe study enrolled 169 female SLE patients and 132 unrelated female healthy individuals. Baseline clinical, laboratorial characteristics, and the SLE activity (determined using the SLEDAI) were evaluated according to the CCR5Δ32genotypes. The CCR5Δ32 polymorphism was determined from genomic DNA using a polymerase chain reaction.ResultsThe frequencies of the genotypes CCR5/CCR5, CCR5/CCR5Δ32 and CCR5Δ32/CCR5Δ32 were 87.6%, 11.8%, and 0.6%, respectively, among the patients, and 96.2%, 3.8%, and 0.0%, respectively among the controls, [p=0.0116, odds ratio:3.432 (95% confidence interval:1.252–9.40). Patients carrying the CCR5/CCR5Δ32 and CCR5Δ32/CCR5Δ32 genotypes presented earlier age of onset of disease (p=0.0293) and higher levels of anti-dsDNA (p=0.0255), than those carrying the wild type genotype. When the analysis was adjusted for ethnicity, only the age at onset of disease remained associated with the CCR5Δ32 polymorphism (p