Anti-tumor activity of polysaccharides extracted from Senecio scandens Buch, -Ham root on hepatocellular carcinoma
Purpose: To optimize the extraction conditions of polysaccharides from the root of Senecio scandens Buch,-Ham. (PRS) and evaluate its anti-tumor effect on hepatocellular carcinoma. Methods: Response surface methodology (RSM) applied with a Box-Behnken design (BBD, three levels and three factors) was...
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Veröffentlicht in: | Tropical journal of pharmaceutical research 2019-07, Vol.16 (1) |
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Sprache: | eng |
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Zusammenfassung: | Purpose: To optimize the extraction conditions of polysaccharides from
the root of Senecio scandens Buch,-Ham. (PRS) and evaluate its
anti-tumor effect on hepatocellular carcinoma. Methods: Response
surface methodology (RSM) applied with a Box-Behnken design (BBD, three
levels and three factors) was employed to determine the effect of
extraction time, number of extraction and ratio of water to raw
material on the yield of PRS. The anti-tumor effect of PRS on A549,
HL60, S180 and H22 cell lines was evaluated in vitro by
3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT)
assay, while in vivo anti-tumor effect was evaluated in H22 tumor
transplanted mice. Furthermore, expressions of proteins including
caspase-3, caspase-9, Bcl-2 and Bax were determined by western blotting
assay. Results: The established BBD model was highly significant and
the optimal conditions were: extraction time, 3.06 h; number of
extractions, 2; and ratio of water to raw material, 16.17 mL/g. PRS
showed significant inhibitory effect on H22 cells (IC50 = 42.4
μg/mL), and significantly inhibited the growth of transplanted H22
tumors in mice at the doses of 20, 40 and 80 mg/kg (p < 0.05, p <
0.05 and p < 0.01, respectively). Treatment with PRS (20, 40 and 80
μg/mL) significantly up-regulated the expressions of Bax,
caspase-3 and caspase-9 in H22 cells, whereas Bcl-2 protein was
significantly down-regulated. Conclusion: The results suggest that PRS
possesses significant anti-tumor activity on H22 cell line in vitro and
in vivo, and the mechanism may be closely related to the induction of
mitochondria-mediated apoptosis. |
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ISSN: | 1596-5996 |