ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy
BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae . Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. le...
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description | BACKGROUND Leprosy is a chronic infectious disease caused by the
obligate intracellular bacillus Mycobacterium leprae . Because leprosy
diagnosis is complex and requires professional expertise, new tools and
methodologies are needed to detect cases in early stages and prevent
transmission. The M. leprae genome contains mce1A, which encodes a
putative mammalian cell entry protein (Mce1A). We hypothesised that the
presence of Mce1A on the cell surface could be detected by the
host's immune system. OBJECTIVE The aim of this study was to
evaluate antibody responses against the Mce1A protein in leprosy
patients, household contacts of patients, and the general population to
present an addition tool for leprosy diagnosis. METHODS A
cross-sectional study involving 89 volunteers [55 leprosy cases, 12
household contacts (HHC) and 22 endemic controls (EC)] was conducted at
Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The
median anti-Mce1A IgA was significantly higher in multibacillary (MB)
and paucibacillary (PB) cases than in EC (p < 0.0001). A similar
trend was observed in IgM levels, which were significantly higher in
both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC
individuals. The greatest differences were observed for IgG
class-specific antibodies against Mce1A. The median levels of MB and PB
were significantly higher compared to both controls HHC and EC (MB or
PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among
leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and
specificity were 92.7% and 97.1%, respectively. IgG positivity was
confirmed in 92.1% and 94.1% of MB and PB patients, respectively.
CONCLUSION This novel diagnostic approach presents an easy,
non-invasive, and inexpensive method for leprosy screening, which may
be applicable in endemic areas. |
format | Article |
fullrecord | <record><control><sourceid>bioline</sourceid><recordid>TN_cdi_bioline_primary_cria_bioline_oc_oc17122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_oc_oc17122</sourcerecordid><originalsourceid>FETCH-bioline_primary_cria_bioline_oc_oc171223</originalsourceid><addsrcrecordid>eNqVjs1KxEAQhOeg4PrzDn3UQySTlWTxFmT9Afek99CZdGLLzHSYnhXyCL61OegDCAVFfUVBnZiNrZtdsSvr8sycq36WZdVs67uN-d6_vry1RY9KA6AqLiAjcAjHKJOX_ug5whNgzNzLwKSAE3LUDAFDQM8YwZH3QDGnBWwL1wdHtr2BOUkmjveAEOWLPAyMUxTN7ADntUX3AaMk8LQGXS7N6Yhe6erXL8zt4_794bnoWdYT1M2JA6alc4mx-4PiVtnGVtX234MfwL9coA</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</title><source>Bioline International</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Lima, Filipe R ; Takenami, Iukary ; Cavalcanti, Maurílio AL ; Riley, Lee W ; Arruda, Sérgio</creator><creatorcontrib>Lima, Filipe R ; Takenami, Iukary ; Cavalcanti, Maurílio AL ; Riley, Lee W ; Arruda, Sérgio</creatorcontrib><description>BACKGROUND Leprosy is a chronic infectious disease caused by the
obligate intracellular bacillus Mycobacterium leprae . Because leprosy
diagnosis is complex and requires professional expertise, new tools and
methodologies are needed to detect cases in early stages and prevent
transmission. The M. leprae genome contains mce1A, which encodes a
putative mammalian cell entry protein (Mce1A). We hypothesised that the
presence of Mce1A on the cell surface could be detected by the
host's immune system. OBJECTIVE The aim of this study was to
evaluate antibody responses against the Mce1A protein in leprosy
patients, household contacts of patients, and the general population to
present an addition tool for leprosy diagnosis. METHODS A
cross-sectional study involving 89 volunteers [55 leprosy cases, 12
household contacts (HHC) and 22 endemic controls (EC)] was conducted at
Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The
median anti-Mce1A IgA was significantly higher in multibacillary (MB)
and paucibacillary (PB) cases than in EC (p < 0.0001). A similar
trend was observed in IgM levels, which were significantly higher in
both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC
individuals. The greatest differences were observed for IgG
class-specific antibodies against Mce1A. The median levels of MB and PB
were significantly higher compared to both controls HHC and EC (MB or
PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among
leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and
specificity were 92.7% and 97.1%, respectively. IgG positivity was
confirmed in 92.1% and 94.1% of MB and PB patients, respectively.
CONCLUSION This novel diagnostic approach presents an easy,
non-invasive, and inexpensive method for leprosy screening, which may
be applicable in endemic areas.</description><identifier>ISSN: 1678-8060</identifier><language>eng</language><publisher>Fundação Oswaldo Cruz, Fiocruz</publisher><subject>antibodies ; diagnosis ; immunoglobulin ; leprosy ; Mce1A protein</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2019-05, Vol.112 (12)</ispartof><rights>Copyright 2017 - Memórias do Instituto Oswaldo Cruz</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,79395</link.rule.ids></links><search><creatorcontrib>Lima, Filipe R</creatorcontrib><creatorcontrib>Takenami, Iukary</creatorcontrib><creatorcontrib>Cavalcanti, Maurílio AL</creatorcontrib><creatorcontrib>Riley, Lee W</creatorcontrib><creatorcontrib>Arruda, Sérgio</creatorcontrib><title>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</title><title>Memórias do Instituto Oswaldo Cruz</title><description>BACKGROUND Leprosy is a chronic infectious disease caused by the
obligate intracellular bacillus Mycobacterium leprae . Because leprosy
diagnosis is complex and requires professional expertise, new tools and
methodologies are needed to detect cases in early stages and prevent
transmission. The M. leprae genome contains mce1A, which encodes a
putative mammalian cell entry protein (Mce1A). We hypothesised that the
presence of Mce1A on the cell surface could be detected by the
host's immune system. OBJECTIVE The aim of this study was to
evaluate antibody responses against the Mce1A protein in leprosy
patients, household contacts of patients, and the general population to
present an addition tool for leprosy diagnosis. METHODS A
cross-sectional study involving 89 volunteers [55 leprosy cases, 12
household contacts (HHC) and 22 endemic controls (EC)] was conducted at
Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The
median anti-Mce1A IgA was significantly higher in multibacillary (MB)
and paucibacillary (PB) cases than in EC (p < 0.0001). A similar
trend was observed in IgM levels, which were significantly higher in
both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC
individuals. The greatest differences were observed for IgG
class-specific antibodies against Mce1A. The median levels of MB and PB
were significantly higher compared to both controls HHC and EC (MB or
PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among
leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and
specificity were 92.7% and 97.1%, respectively. IgG positivity was
confirmed in 92.1% and 94.1% of MB and PB patients, respectively.
CONCLUSION This novel diagnostic approach presents an easy,
non-invasive, and inexpensive method for leprosy screening, which may
be applicable in endemic areas.</description><subject>antibodies</subject><subject>diagnosis</subject><subject>immunoglobulin</subject><subject>leprosy</subject><subject>Mce1A protein</subject><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNqVjs1KxEAQhOeg4PrzDn3UQySTlWTxFmT9Afek99CZdGLLzHSYnhXyCL61OegDCAVFfUVBnZiNrZtdsSvr8sycq36WZdVs67uN-d6_vry1RY9KA6AqLiAjcAjHKJOX_ug5whNgzNzLwKSAE3LUDAFDQM8YwZH3QDGnBWwL1wdHtr2BOUkmjveAEOWLPAyMUxTN7ADntUX3AaMk8LQGXS7N6Yhe6erXL8zt4_794bnoWdYT1M2JA6alc4mx-4PiVtnGVtX234MfwL9coA</recordid><startdate>20190510</startdate><enddate>20190510</enddate><creator>Lima, Filipe R</creator><creator>Takenami, Iukary</creator><creator>Cavalcanti, Maurílio AL</creator><creator>Riley, Lee W</creator><creator>Arruda, Sérgio</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><scope>RBI</scope></search><sort><creationdate>20190510</creationdate><title>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</title><author>Lima, Filipe R ; Takenami, Iukary ; Cavalcanti, Maurílio AL ; Riley, Lee W ; Arruda, Sérgio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-bioline_primary_cria_bioline_oc_oc171223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>antibodies</topic><topic>diagnosis</topic><topic>immunoglobulin</topic><topic>leprosy</topic><topic>Mce1A protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima, Filipe R</creatorcontrib><creatorcontrib>Takenami, Iukary</creatorcontrib><creatorcontrib>Cavalcanti, Maurílio AL</creatorcontrib><creatorcontrib>Riley, Lee W</creatorcontrib><creatorcontrib>Arruda, Sérgio</creatorcontrib><collection>Bioline International</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima, Filipe R</au><au>Takenami, Iukary</au><au>Cavalcanti, Maurílio AL</au><au>Riley, Lee W</au><au>Arruda, Sérgio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><date>2019-05-10</date><risdate>2019</risdate><volume>112</volume><issue>12</issue><issn>1678-8060</issn><abstract>BACKGROUND Leprosy is a chronic infectious disease caused by the
obligate intracellular bacillus Mycobacterium leprae . Because leprosy
diagnosis is complex and requires professional expertise, new tools and
methodologies are needed to detect cases in early stages and prevent
transmission. The M. leprae genome contains mce1A, which encodes a
putative mammalian cell entry protein (Mce1A). We hypothesised that the
presence of Mce1A on the cell surface could be detected by the
host's immune system. OBJECTIVE The aim of this study was to
evaluate antibody responses against the Mce1A protein in leprosy
patients, household contacts of patients, and the general population to
present an addition tool for leprosy diagnosis. METHODS A
cross-sectional study involving 89 volunteers [55 leprosy cases, 12
household contacts (HHC) and 22 endemic controls (EC)] was conducted at
Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The
median anti-Mce1A IgA was significantly higher in multibacillary (MB)
and paucibacillary (PB) cases than in EC (p < 0.0001). A similar
trend was observed in IgM levels, which were significantly higher in
both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC
individuals. The greatest differences were observed for IgG
class-specific antibodies against Mce1A. The median levels of MB and PB
were significantly higher compared to both controls HHC and EC (MB or
PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among
leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and
specificity were 92.7% and 97.1%, respectively. IgG positivity was
confirmed in 92.1% and 94.1% of MB and PB patients, respectively.
CONCLUSION This novel diagnostic approach presents an easy,
non-invasive, and inexpensive method for leprosy screening, which may
be applicable in endemic areas.</abstract><pub>Fundação Oswaldo Cruz, Fiocruz</pub></addata></record> |
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source | Bioline International; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
subjects | antibodies diagnosis immunoglobulin leprosy Mce1A protein |
title | ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy |
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