ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy

BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae . Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. le...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Memórias do Instituto Oswaldo Cruz 2019-05, Vol.112 (12)
Hauptverfasser: Lima, Filipe R, Takenami, Iukary, Cavalcanti, Maurílio AL, Riley, Lee W, Arruda, Sérgio
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 12
container_start_page
container_title Memórias do Instituto Oswaldo Cruz
container_volume 112
creator Lima, Filipe R
Takenami, Iukary
Cavalcanti, Maurílio AL
Riley, Lee W
Arruda, Sérgio
description BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae . Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p < 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.
format Article
fullrecord <record><control><sourceid>bioline</sourceid><recordid>TN_cdi_bioline_primary_cria_bioline_oc_oc17122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_oc_oc17122</sourcerecordid><originalsourceid>FETCH-bioline_primary_cria_bioline_oc_oc171223</originalsourceid><addsrcrecordid>eNqVjs1KxEAQhOeg4PrzDn3UQySTlWTxFmT9Afek99CZdGLLzHSYnhXyCL61OegDCAVFfUVBnZiNrZtdsSvr8sycq36WZdVs67uN-d6_vry1RY9KA6AqLiAjcAjHKJOX_ug5whNgzNzLwKSAE3LUDAFDQM8YwZH3QDGnBWwL1wdHtr2BOUkmjveAEOWLPAyMUxTN7ADntUX3AaMk8LQGXS7N6Yhe6erXL8zt4_794bnoWdYT1M2JA6alc4mx-4PiVtnGVtX234MfwL9coA</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</title><source>Bioline International</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Lima, Filipe R ; Takenami, Iukary ; Cavalcanti, Maurílio AL ; Riley, Lee W ; Arruda, Sérgio</creator><creatorcontrib>Lima, Filipe R ; Takenami, Iukary ; Cavalcanti, Maurílio AL ; Riley, Lee W ; Arruda, Sérgio</creatorcontrib><description>BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae . Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p &lt; 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p &lt; 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p &lt; 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.</description><identifier>ISSN: 1678-8060</identifier><language>eng</language><publisher>Fundação Oswaldo Cruz, Fiocruz</publisher><subject>antibodies ; diagnosis ; immunoglobulin ; leprosy ; Mce1A protein</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2019-05, Vol.112 (12)</ispartof><rights>Copyright 2017 - Memórias do Instituto Oswaldo Cruz</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,79395</link.rule.ids></links><search><creatorcontrib>Lima, Filipe R</creatorcontrib><creatorcontrib>Takenami, Iukary</creatorcontrib><creatorcontrib>Cavalcanti, Maurílio AL</creatorcontrib><creatorcontrib>Riley, Lee W</creatorcontrib><creatorcontrib>Arruda, Sérgio</creatorcontrib><title>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</title><title>Memórias do Instituto Oswaldo Cruz</title><description>BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae . Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p &lt; 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p &lt; 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p &lt; 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.</description><subject>antibodies</subject><subject>diagnosis</subject><subject>immunoglobulin</subject><subject>leprosy</subject><subject>Mce1A protein</subject><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNqVjs1KxEAQhOeg4PrzDn3UQySTlWTxFmT9Afek99CZdGLLzHSYnhXyCL61OegDCAVFfUVBnZiNrZtdsSvr8sycq36WZdVs67uN-d6_vry1RY9KA6AqLiAjcAjHKJOX_ug5whNgzNzLwKSAE3LUDAFDQM8YwZH3QDGnBWwL1wdHtr2BOUkmjveAEOWLPAyMUxTN7ADntUX3AaMk8LQGXS7N6Yhe6erXL8zt4_794bnoWdYT1M2JA6alc4mx-4PiVtnGVtX234MfwL9coA</recordid><startdate>20190510</startdate><enddate>20190510</enddate><creator>Lima, Filipe R</creator><creator>Takenami, Iukary</creator><creator>Cavalcanti, Maurílio AL</creator><creator>Riley, Lee W</creator><creator>Arruda, Sérgio</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><scope>RBI</scope></search><sort><creationdate>20190510</creationdate><title>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</title><author>Lima, Filipe R ; Takenami, Iukary ; Cavalcanti, Maurílio AL ; Riley, Lee W ; Arruda, Sérgio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-bioline_primary_cria_bioline_oc_oc171223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>antibodies</topic><topic>diagnosis</topic><topic>immunoglobulin</topic><topic>leprosy</topic><topic>Mce1A protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima, Filipe R</creatorcontrib><creatorcontrib>Takenami, Iukary</creatorcontrib><creatorcontrib>Cavalcanti, Maurílio AL</creatorcontrib><creatorcontrib>Riley, Lee W</creatorcontrib><creatorcontrib>Arruda, Sérgio</creatorcontrib><collection>Bioline International</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima, Filipe R</au><au>Takenami, Iukary</au><au>Cavalcanti, Maurílio AL</au><au>Riley, Lee W</au><au>Arruda, Sérgio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><date>2019-05-10</date><risdate>2019</risdate><volume>112</volume><issue>12</issue><issn>1678-8060</issn><abstract>BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae . Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p &lt; 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p &lt; 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p &lt; 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.</abstract><pub>Fundação Oswaldo Cruz, Fiocruz</pub></addata></record>
fulltext fulltext
identifier ISSN: 1678-8060
ispartof Memórias do Instituto Oswaldo Cruz, 2019-05, Vol.112 (12)
issn 1678-8060
language eng
recordid cdi_bioline_primary_cria_bioline_oc_oc17122
source Bioline International; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects antibodies
diagnosis
immunoglobulin
leprosy
Mce1A protein
title ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T10%3A50%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-bioline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ELISA-based%20assay%20of%20immunoglobulin%20G%20antibodies%20against%20mammalian%20cell%20entry%201A%20(Mce1A)%20protein:%20a%20novel%20diagnostic%20approach%20for%20leprosy&rft.jtitle=Mem%C3%B3rias%20do%20Instituto%20Oswaldo%20Cruz&rft.au=Lima,%20Filipe%20R&rft.date=2019-05-10&rft.volume=112&rft.issue=12&rft.issn=1678-8060&rft_id=info:doi/&rft_dat=%3Cbioline%3Ecria_bioline_oc_oc17122%3C/bioline%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true