A bacterial artificial chromosome library for Biomphalaria glabrata , intermediate snail host of Schistosoma mansoni
To provide a novel resource for analysis of the genome of Biomphalaria glabrata , members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Ins...
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Veröffentlicht in: | Memórias do Instituto Oswaldo Cruz 2007-01, Vol.101 |
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Sprache: | eng |
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Zusammenfassung: | To provide a novel resource for analysis of the genome of Biomphalaria
glabrata , members of the international Biomphalaria glabrata Genome
Initiative (biology.unm.edu/biomphalaria-genome.html), working with the
Arizona Genomics Institute (AGI) and supported by the National Human
Genome Research Institute (NHGRI), produced a high quality bacterial
artificial chromosome (BAC) library. The BB02 strain B. glabrata , a
field isolate (Belo Horizonte, Minas Gerais, Brasil) that is
susceptible to several strains of Schistosoma mansoni , was selfed for
two generations to reduce haplotype diversity in the offspring. High
molecular weight DNA was isolated from ovotestes of 40 snails,
partially digested with HindIII, and ligated into pAGIBAC1 vector. The
resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones
(136.3 kb average insert size) and provides 9.05 ´ coverage of the
931 Mb genome. Probing with single/low copy number genes from B.
glabrata and fingerprinting of selected BAC clones indicated that the
BAC library sufficiently represents the gene complement. BAC end
sequence data (514 reads, 299860 nt) indicated that the genome of B.
glabrata contains ~ 63% AT, and disclosed several novel genes,
transposable elements, and groups of high frequency sequence elements.
This BG_BBa BAC library, available from AGI at cost to the research
community, gains in relevance because BB02 strain B. glabrata is
targeted whole genome sequencing by NHGRI. |
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ISSN: | 1678-8060 |