Identification of Strains Isolated in Thailand and Assigned to the Genera Kozakia and Swaminathania

Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora (,,khem" in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from...

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Veröffentlicht in:Journal of culture collections 2010-05, Vol.6 (1)
Hauptverfasser: Kommanee, Jintana, Tanasupawat, Somboon, Akaracharanya, Ancharida, Malimas, Taweesak, Yukphan, Pattaraporn, Muramatsu, Yuki, Nakagawa, Yasuyoshi, Yamada, Yuzo
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Zusammenfassung:Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora (,,khem" in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO3-containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO3 (w/v). Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % Dglucose (w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates SI15-1 and SI15-2 were unequivocally identified respectively as Kozakia baliensis and Swaminathania salitolerans . Изследвани са таксономично четири щама, изолирани съответно от плод на саподила в Чантабури, означени като CT8-1 и CT8-2, и от семена на иксора (,,кем" на тайвански) в Районг и означени като SI15-1 and SI15-2. Че- тирите щама са подбрани от общо 181 изолата на оцетнокисели бактерии. Щамове CT8-1 и CT8-2 са неподвижни и продуцират леваноподобен муко- полизахарид от захароза или D-фруктоза, но не отделят водноразтворим кафяв пигмент от D-глюкоза върху скосен агар, съдържащ CaCO3. Изола- тите произвеждат оцетна киселина от етанол и окисляват ацетат и лактат до въглероден двуокис и вода, но интензивността на окислението е слаба. Техният растеж не се инхибира от 0.35 % оцетна киселина (v/v) при pH 3.5. Изолатите не растат върху 30 % D-глюкоза (w/v), а усвояване на метанол не се установява. Щамове SI15-1 и SI15-2 имат перитрихално разположени ресни и растат в присъствие както на 0.35 % оцетна киселина (v/v) при pH 3.5, така и на 3 % NaCl (w/v) или 1 % KNO3 (w/v). Ацетатът и лактатът се окисляват д
ISSN:1310-8360