Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

Coumarin-based fluorescent probe for the detection of glutathione and nitroreductase

With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluo...

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Veröffentlicht in:Tetrahedron 2021-02, Vol.82, Article 131890
Hauptverfasser: Tian, Xue, Kumawat, Lokesh K., Bull, Steven D., Elmes, Robert B. P., Wu, Luling, James, Tony D.
Format: Artikel
Sprache:eng
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Zusammenfassung:With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe NTR-AHC was constructed by attaching the NTR trigger moiety (p-nitrobenzyl) and biothiol acceptor (maleic acid monoamide), to the core fluorophore AHC. In the presence of both glutathione (GSH) and NTR, probe NTR-AHC produced a >8-fold increase in fluorescence intensity at 463 nm.
ISSN:0040-4020
1464-5416
DOI:10.1016/j.tet.2020.131890