Interaction between DNA and the Hfq amyloid-like region triggers a viscoelastic response

Molecular transport of biomolecules plays a pivotal role in the machinery of life. Yet, this role is poorly understood due the lack of quantitative information. Here, the role and properties of the C-terminal region of Escherichia coli Hfq is reported, involved in controlling the flow of a DNA solut...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:arXiv.org 2020-07
Hauptverfasser: Omar El Hamoui, Yadav, Indresh, Radiom, Milad, Wien, Frank, Berret, Jean-Francois, Johan R C van der Maarel, Arluison, Véronique
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Molecular transport of biomolecules plays a pivotal role in the machinery of life. Yet, this role is poorly understood due the lack of quantitative information. Here, the role and properties of the C-terminal region of Escherichia coli Hfq is reported, involved in controlling the flow of a DNA solution. A combination of experimental methodologies has been used to probe the interaction of Hfq with DNA and to measure the rheological properties of the complex. A physical gel with a temperature reversible elasticity modulus is formed due to formation of non-covalent crosslinks. The mechanical response of the complexes shows that they are inhomogeneous soft solids. Our experiments indicate that Hfq C-terminal region could contribute to genome mechanical response. The reported viscoelasticity of the DNA-protein complex might have implications for cellular pro-cesses involving molecular transport of DNA or segments thereof.
ISSN:2331-8422
DOI:10.48550/arxiv.2007.14663