Changes in respiration and excretion rates of boreal zooplankton after capture (Saanich Inlet, British Columbia, Canada)

Maintenance and Update Frequency: notPlanned | Statement: Statement: The method used to measure respiration rate is described in: Ikeda T (1976) The effect of laboratory conditions on the extrapolation of experimental measurements to the ecology of marine zooplankton. I. Effect of feeding condition on the respiration rate. Bull. Plankton Soc. Japan 23, 1-10.Ammonia and inorganic phosphate were determined by methods described in:Strickland JDH and Parsons TR (1972) A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can. 167, 1-310.The results for days 34 and 49 for Calanus plumchrus were compromised due to the presence of epiphytic pennate diatoms on the body surface of starved specimens at day 34 and on both fed and starved individuals by day 49. The implications of the presence of epiphytic pennate diatoms is discussed in:Ikeda T (1977) A pelagic marine copepod associated with diatoms. Bulletin of the Plankton Society of Japan 24: 115-118.The starved replicates were not successful for Parathemisto pacifica because cannibalism occurred in all but 1 replicate as soon as the experiment began. Young larvae appeared in one of the fed replicates of Parathemisto pacifica on day 4. A large number of larvae was produced in all replicates of fed Pleurobrachia pileus from day 5. | Credit Ikeda, Tsutomu, Dr (Principal Investigator) | The herbivorous copepods, Calanus plumchrus and Paracalanus parvus, the herbivorous euphausiid, Euphausia pacifica, the carnivorous amphipod, Parathemisto pacifica and the carnivorous ctenophore, Pleurobrachia pileus used in this experiment were collected from Saanich Inlet, British Columbia, Canada, between May and August 1975.All animals, except for Paracalanus parvus, which were used immediately, were left overnight in unfiltered sea water collected from the same site as the zooplankton. Zooplankton were then maintained in 300 to 500 ml beakers containing well aerated filtered seawater in a constant temperature room at 10°C ± 1°C. Two different feeding regimes were used for the experiment, with one group of each species fed excess food and the other starved by placing the zooplankton in filtered sea water only. Calanus plumchrus, Paracalanus parvus and Euphausia pacifica were fed a diet of cultured Chaetoceros spp., originally collected from the same site as the zooplankton. Parathemisto pacifica were fed chopped fresh Euphausia pacifica and Pleurobrachia pileus were fed freshly collected Calanus plumchrus.Prior to startin