SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in November 2021

Maintenance and Update Frequency: asNeeded | Statement: Since May 2014 water samples were taken at each station at three depths; 5m, DCM and 10m below DCM. In vivo CTD profiles were used to identify the temperature, salinity and fluorescence profiles of the water column, ultimately determining the depth of the Deep Chlorophyll Maximum (DCM). Niskin bottles were used for seawater sampling for nutrients, chlorophyll a, Pico phytoplankton, bacteria and virus analysis and Particulate Inorganic Matter, Particulate Organic Matter, Total Suspended Sediments, and phytoplankton community compositions and abundances. If no DCM could be identified, seawater sampling was done at or near the thermocline interface. Note - from September 2017, "Particulate Inorganic Matter, Particulate Organic Matter, Total Suspended Sediments" was discontinued for now. Seawater samples of 50-70 mL were filtered through bonnet syringe filters (0.45 um porosity, Micro Analytix Pty Ltd) and stored at –20oC for nutrient analysis. Dissolved ammonium (NH3, detection limit 0.071 µM), oxides of nitrogen (NOx (NO2 + NO3), detection limit 0.071 µM), phosphate (PO4, detection limit 0.032 µM) and silicate (SiO2, detection limit 0.333 µM), were determined by flow injection analysis with a QuickChem 8500 Automated Ion Analyser. Chlorophyll a concentrations were determined by filtering 2L seawater samples through stacked 5um mesh and pre-combusted glass fibre filter (Whatman GF/F, nominal pore size 0.7 um porosity). Filterswere stored in cryovials and frozen in liquid nitrogen in the field. Samples were stored at -80oC until analysis. Chlorophyll a was analysed via High Performance Liquid Chromatography using anAgilent LC1260 HPLC with a photodiode array detector and a refrigerated autosampler. Since May 2014 SAIMOS has analysed chlorophyll pigments using HPLC techniques. The data being supplied is slightly processed (reformatted really) to show similar results to the previous spectrophotometry analyse method. For picophytoplankton, triplicate 1 ml samples were added to cryovials which were pre-spiked with 10 ul glutaraldehyde (25% EM grade). For bacteria and viruses, triplicate 1 ml samples were added to cryovials which were pre-spiked with 20 ul glutaraldehyde (25% EM grade). For bacteria and viruses, triplicateSamples were fixed for 10-15 minutes in the dark in the fridge (4oC) before being submerged in liquid nitrogen. Samples were then stored at -80oC in the laboratory until analysed by flow cyto