Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen
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| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
Hamburg
[2021]
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| 245 | 1 | 0 | |a Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen |c vorgelegt von: Kristen Kei Thomsen |
| 264 | 1 | |a Hamburg |c [2021] | |
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Datensatz im Suchindex
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| adam_text | INHALTSVERZEICHNIS
1
ARBEITSHYPOTHESE
UND
FRAGESTELLUNG
.........................................................................................
1
2
EINLEITUNG
UND
STAND
DER
FORSCHUNG
.........................................................................................
3
2.1
DER
HIPPOCAMPUS
................................................................................................................
3
2.1.1
DIE
FUNKTION
DES
HIPPOCAMPUS
................................................................................
4
2.1.2
DIE
ANATOMIE
DER
HIPPOCAMPALEN
FORMATION
.........................................................
5
2.1.3
DER
GYRUS
DENTATUS
....................................................................................................
7
2.1.3.1
STRATUM
MOLEKULARE
......................................................................................
7
2.1.3.2
STRATUM
GRANULAERE
..........................................................................................
8
2.1.3.3
POLYMORPHE
ZELLSCHICHT
................................................................................
8
2.1.4
DIE
KOEMERZELLEN
IM
STRATUM
GRANULAERE
DES
GYRUS
DENTATUS
..................................
9
2.1.4.1
DIE
MORPHOLOGIE
DER
KOEMERZELLEN
.............................................................
9
2.1.4.2
DIE
NEUROGENESE
DER
KOEMERZELLEN
...........................................................
10
2.1.4.3
EIGENSCHAFTEN
UND
FUNKTIONEN
DER
KOEMERZELLEN
.....................................
12
2.2
DER
SPINE
APPARAT
UND
DIE
YYCISTEMAL
ORGANELLE
..........................................................
14
2.2.1
DENDRITISCHE
SPINES
................................................................................................
14
2.2.2
DAS
PROTEIN
SYNAPTOPODIN
.....................................................................................
15
2.2.3
DER
SPINE
APPARAT
..................................................................................................
17
2.2.4
DAS
AXONALE
INITIALSEGMENT
...................................................................................
19
2.2.5
DIE
YYCISTEMAL
ORGANELLE
......................................................................................
19
2.2.6
SYNAPTOPODIN-KNOCKOUT
MAEUSE
...........................................................................
20
2.3
CALCIUM
IN
NEURONEN
UND
DENDRITISCHEN
SPINES
.............................................................
21
2.4
SYNAPTISCHE
PLASTIZITAET
UND
LANGZEITPOTENZIERUNG
..........................................................
23
2.5
DIE
PATCH-CLAMP-TECHNIK
.................................................................................................25
2.5.1
CE7/-.4//AC/2E 7-ABLEITUNGEN
......................................................................................26
2.5.2
IF7ZO/E-CE//-ABLEITUNGEN
..........................................................................................27
2.5.3
DER
EINFLUSS
DER
PIPETTE
...........................................................................................28
3
MATERIAL
UND
METHODE
..............................................................................................................29
3.1
GEWEBEKULTUR
..................................................................................................................
29
3.1.1
VERSUCHSTIERE
...........................................................................................................
29
3.1.2
PRAEPARATION
...............................................................................................................29
3.1.3
INKUBATION
.................................................................................................................
30
3.1.4
REZEPTE
GEWEBEKULTUR
...........................................................................................
31
3.2
ELEKTROPHYSIOLOGIE
...........................................................................................................
31
3.2.1
ALLGEMEINER
VERSUCHSABLAUF
..................................................................................
31
3.2.1.1
KAPAZITAETSKORREKTUR
....................................................................................
33
3.2.1.2
DATENAUFNAHME
...........................................................................................
33
3.2.2
VORVERSUCHE
..............................................................................................................
35
3.2.2.1
77G7Z/-SEA/-ABLEITUNGEN
IM
CELL-ATTACHED-MODUS
..................................
35
3.2.2.2
KONVENTIONELLE
LOOSE-SEAL-ABLEITUNGEN
IM
CELL-ATTACHED-MODUS.
..
.35
3.2.2.3
WHOLE-CELL-ABLEITUNGEN
..............................................
37
3.2.2.4
CELL-ATTACHED
DOUBLE-PATCH
.....................................................................37
3.2.3
LOOSE-SEAL-ABLEITXINGEN
IM
CELL-ATTACHED-ALODUS
MIT
ALBUMIN
IN
DER
ECF.
.
.38
3.2.4
DATENAUSWERTUNG
IN
LABVIEW
................................................................................
39
3.2.5
REZEPTE
ELEKTROPHYSIOLOGIE
....................................................................................
40
4
ERGEBNISSE
...............................................................................................................................
42
4.1
VORVERSUCHE
.....................................................................................................................
42
4.1.1
TIGHT-SEAL-ABLEITUNGEN
IM
CELL-ATTACHED-ALODUS
...............................................
42
4.1.2
KONVENTIONELLE
LOOSE-SEAL-ABLEITUNGEN
IM
CELL-ATTACHED-ALODUS
..................
44
4.1.3
WHOLE-CELL-A AEITUNGEN
.........................................................................................45
4.1.4
CELL-ATTACHEDDOUBLE-PATCH
..................................................................................
46
4.2
LOOSE-SEAL-ABLEITUNGEN
IM
CELL-ATTACHED-MODUS
MIT
ALBUMIN
IN
DER
EXTRAZELLULAERLOESUNG
..........................................................................................................
47
4.2.1
DIE
QUANTITAET
DER
AKTIONSPOTENTIALE
.....................................................................
48
4.2.2
DIE
HALBWERTSBREITE
DER
AKTIONSPOTENTIALE
...........................................................50
4.2.3
DIE
INTER-AKTIONSPOTENTIAL-INTERVALLE
....................................................................
52
5
DISKUSSION
...............................................................................................................................
54
5.1
CELL-ATTACHED-ABLEITUNGEN
AN
HIPPOCAMPALEN
KOEMERZELLEN
.....................................54
5.1.1
TECHNISCHER
ANSATZ
UND
YYPROOF
OF
CONCEPT
.......................................................54
5.1.2
ECF
VS.
ICF
..............................................................................................................
57
5.1.3
TEMPERATUREINFLUESSE
................................................................................................
58
5.1.4
PHYSIOLOGISCHE
EIGENSCHAFTEN
IM
VERLAUF
DER
NEUROGENESE
...............................
58
5.1.5
ORGANOTYPISCHE
SCHNITTKULTUREN
............................................................................
59
5.1.6
DATENERFASSUNG
UND
AUSWAHLKRITERIEN
..................................................................60
5.2
VERGLEICH
DER
NEURONALEN
AKTIVITAET
VON
SYNAPTOPODIN-KNOCKOUT
UND
WILDTYP
KOEMERZELLEN
......................................................................................................................
61
5.2.1
DIE
QUANTITAET
DER
AKTIONSPOTENTIALE
.....................................................................62
5.2.2
DIE
BREITE
DER
AKTIONSPOTENTIALE
...........................................................................
66
5.2.3
DAS
FEUERVERHALTEN
DER
KOEMERZELLEN
...................................................................69
6
ZUSAMMENFASSUNG
....................................................................................................................
71
7
SUMMARY
...................................................................................................................................
72
8
ABKUERZUNGSVERZEICHNIS
...........................................................................................................
73
9
LITERATURVERZEICHNIS
.................................................................................................................
75
10
DANKSAGUNG
...........................................................................................
87
11
LEBENSLAUF.
.............................................................................................................................
88
12
EIDESSTATTLICHE
ERKLAERUNG
.......................................................................................................89
|
| any_adam_object | 1 |
| author | Thomsen, Kristen |
| author_GND | (DE-588)1246114674 |
| author_facet | Thomsen, Kristen |
| author_role | aut |
| author_sort | Thomsen, Kristen |
| author_variant | k t kt |
| building | Verbundindex |
| bvnumber | BV048246514 |
| ctrlnum | (OCoLC)1290849700 (DE-599)DNB1245661566 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048246514 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T09:23:06Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033626889 |
| oclc_num | 1290849700 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 88 Blätter Illustrationen, Diagramme 30 cm |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| record_format | marc |
| spellingShingle | Thomsen, Kristen Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen 4\p Knockout Molekulargenetik (DE-588)4671699-3 gnd 5\p Körnerzelle (DE-588)4390856-1 gnd 6\p Aktionspotenzial (DE-588)4141745-8 gnd 7\p Zelle (DE-588)4067537-3 gnd 8\p Spine (DE-588)4326241-7 gnd |
| subject_GND | (DE-588)4671699-3 (DE-588)4390856-1 (DE-588)4141745-8 (DE-588)4067537-3 (DE-588)4326241-7 (DE-588)4113937-9 |
| title | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen |
| title_auth | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen |
| title_exact_search | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen |
| title_full | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen vorgelegt von: Kristen Kei Thomsen |
| title_fullStr | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen vorgelegt von: Kristen Kei Thomsen |
| title_full_unstemmed | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen vorgelegt von: Kristen Kei Thomsen |
| title_short | Die neuronale Aktivität hippocampaler Körnerzellen in Synaptopodin-Knockout-Mäusen |
| title_sort | die neuronale aktivitat hippocampaler kornerzellen in synaptopodin knockout mausen |
| topic | 4\p Knockout Molekulargenetik (DE-588)4671699-3 gnd 5\p Körnerzelle (DE-588)4390856-1 gnd 6\p Aktionspotenzial (DE-588)4141745-8 gnd 7\p Zelle (DE-588)4067537-3 gnd 8\p Spine (DE-588)4326241-7 gnd |
| topic_facet | Knockout Molekulargenetik Körnerzelle Aktionspotenzial Zelle Spine Hochschulschrift |
| url | https://d-nb.info/1245661566/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033626889&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT thomsenkristen dieneuronaleaktivitathippocampalerkornerzelleninsynaptopodinknockoutmausen |