B cell receptor signaling methods and protocols

This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B ce...

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Weitere Verfasser: Liu, Chaohong ca. 20./21. Jh (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: New York, NY Springer New York 2018
Ausgabe:1st ed. 2018
Schriftenreihe:Methods in Molecular Biology
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Online-Zugang:DE-355
DE-91
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Beschreibung
Zusammenfassung:This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field
Beschreibung:1 Online-Ressource (XI, 262 Seiten) Illustrationen
ISBN:9781493974740
DOI:10.1007/978-1-4939-7474-0