Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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| 100 | 1 | |a Mancinelli, Gloria |d 1991- |e Verfasser |0 (DE-588)1237365678 |4 aut | |
| 245 | 1 | 0 | |a Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree |c vorgelegt von Gloria Mancinelli |
| 264 | 1 | |a Münster |c 2021 | |
| 300 | |a VI, 150 Blätter |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Westfälische Wilhelms-Universität Münster |d 2021 |g Beschränkt für den Austausch | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-032995326 | |
Datensatz im Suchindex
| _version_ | 1819673691163721728 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
PAGE
LIST
OF
ABBREVIATIONS
1
LIST
OF
FIGURES
3
LIST
OF
TABLES
7
1.
INTRODUCTION
8
1.1.
NEUROSCIENCE:
AN
OVERVIEW
8
1.1.1
THE
BRAIN,
A
BASIC
SYNOPSIS
8
1.1.2
THE
HIPPOCAMPUS:
A
MODEL
SYSTEM
IN
NEUROSCIENCE
10
1.1.3
NEURONAL
ARCHITECTURE:
FUNCTION
AND
DEVELOPMENT
15
1.1.3.1
NEURONAL
ARCHITECTURE
15
1.1.3.2
NEURONAL
FUNCTION
AND
SYNAPTIC
TRANSMISSION
17
1.1.3.3
NEURONAL
DEVELOPMENT
21
1.1.3.4
SYNAPSE
FORMATION
VIA
EXPLORATORY
DENDRITIC
FILOPODIA
22
1.1.3.5
FILOPODIA
LIFE
CYCLE
23
1.2.
MEMBRANE
CURVATURE
AND
SHAPE
REMODELLING
MECHANISMS
26
1.2.1
CURVATURE
OF
BIOLOGICAL
MEMBRANES
26
1.2.1.1
LIPID
MEMBRANES
26
1.2.1.2
MEMBRANE
CURVATURE:
INTRACELLULAR
AND
EXTRACELLULAR
FORCES
28
1.2.2
MEMBRANE
CURVATURE
AND
ACTIN
DYNAMICS
33
1.2.2.1
ACTIN
DYNAMICS
FUNDAMENTALS
33
1.2.2.2
ACTIN
DYNAMICS
IN
DENDRITIC
FILOPODIA
36
1.2.3
MEMBRANE
CURVATURE
AND
CURVATURE
SENSITIVE
PROTEINS
37
1.2.3.1
BAR
DOMAINS
AND
MEMBRANE
CURVATURE
38
1.2.3.2
BAR
DOMAINS
AND
ACTIN
DYNAMICS
38
1.2.3.3
BAR
PROTEIN
FUNCTION
AND
EXPRESSION
IN
HCNS
40
2.
RATIONALE
AND
AIM
OF
THE
DISSERTATION
45
GLORIA
MANCINELLI
DOCTORAL
THESIS
3.
MATERIALS
AND
METHODS
46
3.1
CELL
CULTURE
46
3.1.1
PRIMARY
HIPPOCAMPAL
NEURONS
46
3.1.2
NIH
3T3
AND
HELA
CELL
LINES
48
3.2
MOLECULAR
BIOLOGY
48
3.2.1
FLUORESCENTLY
TAGGED
CONSTRUCTS
48
3.2.2
SIRNAS
49
3.2.3
FKBP
AND
MEOS3.2
CONSTRUCTS
50
3.2.4
CLONING
50
3.2.5
SDS
PAGE
AND
WESTERN
BLOT
54
3.3
TRANSFECTION
AND
IMMUNOFLUORESCENCE
58
3.3.1
TRANSFECTION:
LIPOFECTAMINE
2000
58
3.3.2
IMMUNOFLUORESCENCE
59
3.4
FLUORESCENT
MICROSCOPY
60
3.4.1
LIVE
CELL
IMAGING
60
3.4.2
Z
STACKS
ACQUISITIONS
61
3.4.3
TIRF
ACQUISITIONS
61
3.5
SCANNING
ELECTRON
MICROSCOPY
61
3.6
LATTICE
LIGHT
SHEET
MICROSCOPY
61
3.7
PALM
62
3.8
IMAGE
ANALYSIS
62
3.9
STATISTICAL
ANALYSIS
65
4.
RESULTS
66
4.1
FILOPODIA
DYNAMICS
IS
BIASED
TOWARDS
THIN
DISTAL
ENDS
66
4.2
MEMBRANE
CURVATURE
MEDIATES
FILOPODIA
ACTIVATOR
CONCENTRATION
ENRICHMENT
68
IV
GLORIA
MANCINELLI
DOCTORAL
THESIS
4.2.1
CURVATURE
BIOSENSOR
ACCUMULATION
HOLDS
FOR
OTHER
L-BAR
MEMBERS
71
4.3
ENERGETIC
CONSIDERATIONS
ON
FILOPODIA
FORMATION
PREDICT
THE
PRESENCE
OF
AN
OPPOSITE
MEMBRANE
BIOSENSOR
TO
THE
NEGATIVE
DEFORMATION
ACTIVATOR
75
4.4
ACTIVATOR/INHIBITOR
DYNAMICS
IS
REGULATED
ALONG
NEURONAL
ARCHITECTURE
77
4.4.1
BIOSENSORS
OF
CHOICE:
CNS
TISSUE
EXPRESSION
PROFILE
77
4.4.2
MEMBRANE
CURVATURE
REGULATES
ACTIVATOR/INHIBITOR
RATIO
IN
NEURONS
78
4.5
FILOPODIA
INITIATION
IS
TUNED
BY
A
LOCAL
OPPOSITE
CURVATURE
CO-EXISTENCE
81
4.5.1
CLASSIFICATION
OF
DIFFERENT
FILOPODIA
POPULATIONS
TO
ESTABLISH
THE
INITIATION
PHASE
81
4.5.2
BIOSENSORS
OF
OPPOSITE
CURVATURE
AFFINITY
ENRICH
AT
INITIAL
MEMBRANE
DEFORMATION
PRIOR
TO
FILOPODIA
FORMATION
82
4.6
DOWNREGULATION
OF
ACTIVATOR
AND
INHIBITOR
CHANGE
FILOPODIA
FORMATION
IN
THIN
DENDRITIC
PORTION
85
4.6.1
SUPPLEMENT:
ANTIBODY
STAINING
CONTROL
CONFIRMS
SIRNA
DOWNREGULATION
EFFICIENCY
88
4.6.2
SUPPLEMENT:
WESTERN
BLOT
CONTROL
CONFIRMS
SIRNA
DOWNREGULATION
EFFECT
89
4.7
MEMBRANE
DEFORMATION
UPON
L-BAR
INDUCTION
AT
THE
MEMBRANE
TRIGGERS
FILOPODIA
FORMATION
90
4.7.1
MEMBRANE
DEFORMATION
UPON
L-BAR
CID
IS
CONFIRMED
IN
TIRF
AND
IS
SHOWED
TO
INDUCE
ACTIN-CONTAINING
FILOPODIA
IN
NEURONS
92
5.
DISCUSSION
95
5.1
MEMBRANE
CURVATURE
GUIDES
FILOPODIA
DYNAMICS
POSITIONING
ALONG
NEURONAL
ARCHITECTURE
95
5.2
FILOPODIA
CURVATURE-DEPENDENT
ACTIVATOR
DISTRIBUTES
IN
CORRELATION
WITH
CURVATURE
INCREASE
OF
THE
DENDRITIC
TREE
97
5.3
GLOBAL
MEMBRANE
CURVATURE
CONFORMATION
INFLUENCES
LOCAL
CURVATURE-DEPENDENT
EVENTS
99
5.4
FILOPODIA
FORMATION
IS
ALTERED
FOR
ACTIVATOR/INHIBITOR
RATIO
DISTURBANCES
101
V
GLORIA
MANCINELLI
DOCTORAL
THESIS
5.5
NEURONAL
ARCHITECTURE
AN
EXEMPLARY
MODEL
OF
FUNCTIONAL
MORPHOLOGY
103
6.
CONCLUSIONS
109
7.
FUTURE
DIRECTIONS
111
8.
REFERENCES
115
9.
AKNOWLEDGMENTS
138
10.
LEBENSLAUF
140
11.
APPENDIX
142
VI
|
| any_adam_object | 1 |
| author | Mancinelli, Gloria 1991- |
| author_GND | (DE-588)1237365678 |
| author_facet | Mancinelli, Gloria 1991- |
| author_role | aut |
| author_sort | Mancinelli, Gloria 1991- |
| author_variant | g m gm |
| building | Verbundindex |
| bvnumber | BV047610458 |
| ctrlnum | (OCoLC)1260455039 (DE-599)DNB1238190227 |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047610458 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T09:00:04Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032995326 |
| oclc_num | 1260455039 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | VI, 150 Blätter Illustrationen 30 cm |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| record_format | marc |
| spellingShingle | Mancinelli, Gloria 1991- Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree |
| subject_GND | (DE-588)4113937-9 |
| title | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree |
| title_auth | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree |
| title_exact_search | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree |
| title_full | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree vorgelegt von Gloria Mancinelli |
| title_fullStr | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree vorgelegt von Gloria Mancinelli |
| title_full_unstemmed | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree vorgelegt von Gloria Mancinelli |
| title_short | Membrane curvature as intrinsic self-organizing module regulates filopodia formation along the neuronal dendritic tree |
| title_sort | membrane curvature as intrinsic self organizing module regulates filopodia formation along the neuronal dendritic tree |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1238190227/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032995326&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mancinelligloria membranecurvatureasintrinsicselforganizingmoduleregulatesfilopodiaformationalongtheneuronaldendritictree |