Non-Han literature along the Silk Road
1. Across-regional and Local Characteristics of Mythologies: on the Basis of Observing the Lop Museum Carpets -- 2. Legends and Ceremonies: Based on the Observation of the Qu Shu Collection at Xinjiang Lop Museum -- 3. Wang Shuhe Maijue in Iran -- 4. Relation between Sogdiana and Turfan during the 1...
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| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV046899804 | ||
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| 008 | 200915s2020 si a||| |||| 00||| eng d | ||
| 020 | |a 9789811396434 |c Festeinband |9 978-981-13-9643-4 | ||
| 035 | |a (OCoLC)1192483944 | ||
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| 040 | |a DE-604 |b ger |e rda | ||
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| 245 | 1 | 0 | |a Non-Han literature along the Silk Road |c Xiao Li (editor) |
| 264 | 1 | |a Singapore |b Springer |c [2020] | |
| 264 | 4 | |c © 2020 | |
| 264 | 4 | |a [Hong Kong] |b SDX Joint Publishing Company |c [2020] | |
| 300 | |a xiii, 158 Seiten |b Illustrationen, Karte | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Silk Road research series | |
| 520 | 3 | |a 1. Across-regional and Local Characteristics of Mythologies: on the Basis of Observing the Lop Museum Carpets -- 2. Legends and Ceremonies: Based on the Observation of the Qu Shu Collection at Xinjiang Lop Museum -- 3. Wang Shuhe Maijue in Iran -- 4. Relation between Sogdiana and Turfan during the 10th - 11th centuries as reflected in Manichaean Sogdian texts -- 5. Four New Kharoṣṭhī Documents kept in the Xinjiang Uygur Autonomous Region Museum -- 6. A New Kharoṣṭhī Document from Kucha in the Hetian County Museum Collection -- 7. An Examination of Khujand -- 8. The Xinjiang Region Museum Collection – An Interpretation of a Kharoṣṭhī Letter -- 9. A New Transcription and Translation of the Kharoṣṭhī Deed of Divorce in Hetian Museum | |
| 520 | 3 | |a This volume includes outstanding scientific articles on documents written in ancient languages such as Tocharian, Sogdian, Khotanese, and Old Uyghur. Its chief aims are to contribute to the present state of research by adding essential findings on newly discovered historical documents; to present a multi-dimensional investigation of diverse aspects including the history, religion, art, literature, and social life along the Silk Road; and to outline potential future research directions for non-Han literature studies and inspire research into other aspects, such as economics and comparative studies | |
| 648 | 7 | |a Geschichte |2 gnd |9 rswk-swf | |
| 651 | 7 | |a Seidenstraße |0 (DE-588)4054299-3 |2 gnd |9 rswk-swf | |
| 653 | 0 | |a Asia—History | |
| 653 | 0 | |a Religion—History | |
| 653 | 0 | |a Archaeology | |
| 653 | 0 | |a Philology | |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Seidenstraße |0 (DE-588)4054299-3 |D g |
| 689 | 0 | 1 | |a Geschichte |A z |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Li, Xiao |d 1944- |0 (DE-588)1020703741 |4 edt | |
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| 940 | 1 | |q BSB_NED_20210421 | |
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| 942 | 1 | 1 | |c 417.7 |e 22/bsb |g 5 |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-032309483 | |
Datensatz im Suchindex
| _version_ | 1819748692338409472 |
|---|---|
| adam_text | 1
INTRODUCTION
_______________________________________________________
1
1.1
FGF2
SYNTHESIS
AND
STRUCTURE
___________________________________________
1
1.2
THE
UNCONVENTIONAL
SECRETION
OF
FGF2
____________________________________
2
1.3
FGF
RECEPTORS
STRUCTURE
AND
FGF2
RECEPTOR
SPECIFICITY
_____________________
4
1.4
FGF2
SIGNALING
________________________________________________________
5
1.5
FGF2
INTERACTION
PARTNERS
ON
CELL
SURFACES
AND
IN
THE
EXTRACELLULAR
MATRIX
____
6
1.6
BIOLOGICAL
AND
PATHOPHYSIOLOGICAL
ROLES
OF
FGF2
AND
EXAMPLES
OF
ITS MEDICAL
APPLICATIONS
_______________________________________________________________
7
1.7
THE
ROLE
OF
FGF2
AND
FGFR1
IN
PROMOTING
TUMORIGENESIS
___________________
8
1.8
FGF2
AND
FGFR
BASED
ANTI-CANCER
THERAPIES
_____________________________
13
1.9
A
PROAPOPTOTIC
ROLE
OF
FGF2
SIGNALING
IN
SK-N-MC
CELLS
____________________ 16
1.10
ACTIVATION
AND
SUBCELLULAR
LOCALIZATION
OF
ERK
___________________________ 17
1.11
HIGH
THROUGHPUT
SCREENS
IN
DRUG
DISCOVERY
______________________________ 19
2
AIM
OF
THESIS_____________________________________________________
21
3
MATERIAL
AND
METHODS
______________________________________________
22
3.1
MATERIAL
___________________________________________________________ 22
3.1.1
CHEMICALS,
CELL
MEDIA
AND
REAGENTS
_________________________________________ 22
3.1.2
DEVICES
_________________________________________________________________ 23
3.1.3
CONSUMABLES
____________________________________________________________
24
3.1.4
ASSAY
KITS
_______________________________________________________________
24
3.1.5
ANTIBODIES
______________________________________________________________
25
3.1.6
PRIMERS
AND
OLIGONUCLEOTIDES
______________________________________________
25
3.1.7
ENZYMES
________________________________________________________________
25
3.1.8
PLASMIDS
________________________________________________________________
26
3.1.9
SIRNAS
(SMALL
INTERFERING
RNAS)
____________________________________________
26
3.1.10
MAMMALIAN
CEIL
LINES
_____________________________________________________
27
3.1.11
SOFTWARE
TOOLS
USED
FOR
DATA
ANALYSIS
________________________________________
27
3.1.12
CELL
CULTURE
MEDIA
________________________________________________________
27
3.1.13
BACTERIAL
MEDIA
FOR
CLONING
AND
PROTEIN
PRODUCTION
____________________________
28
3.2
METHODS
___________________________________________________________
30
3.2.1
CELL
BIOLOGICAL
METHODS
_____________________________________________________
30
3.2.1.1
MAINTAINING
CELLS
IN
CULTURE
____________________________________________
30
3.2.1.2 SPLITTING
CELLS
_________________________________________________________
30
3.2.1.3
FREEZING
AND
THAWING
MAMMALIAN
CELLS
___________________________________
30
3.2.1.4
GENERATION
OF
SK-N-MCMCHERRY-NLS
STABLE
CELL
LINE
________________________
31
3.2.1.5
FLUORESCENCE
ACTIVATED
CELL
SORTING
(FACS)
________________________________
32
3.2.1.6
SIRNA
TRANSFECTION
OF
HELA
S3
FGF2_IRES_GFP
____________________________
32
3.2.1.7
COCULTURING
EXPERIMENTS
OF
HELA
S3
FGF2_IRES_GFP
AND
SK-N-MCMCHERRY-NLS_
33
3.2.1.8
FGF2
TITRATION
EXPERIMENTS
ON
SK-N-MCMCHERRY-NLS
_______________________
33
3.2.1.9
TREATING
CELLS
WITH INHIBITORS
FOR
SCREENING
_______________________________
33
3.2.1.10
DOSE
RESPONSE
DETERMINATIONS
_______________________________________
33
3.2.1.11
DETECTION
OFP-ERK
IN
WHOLE
CELL
LYSATES
_________________________________
34
3.2.1.12
CELL
VIABILITY
DETERMINATION
USING
THE
ALAMAR
BLUE
ASSAY
_________________
34
3.2.1.13
CELL
VIABILITY
DETERMINATION
USING
THE
MTS
ASSAY
________________________
34
3.2.2
MOLECULAR
BIOLOGY
METHODS
________________________________________________
35
3.2.2.1
DNA
DIGESTION
________________________________________________________
35
3.2.2.2 AGAROSE
GEL
ELECTROPHORESIS
____________________________________________
35
3.2.2.3
DNA
LIGATION
________________________________________________________
36
3.2.2.4
E.
COLI
TRANSFORMATION
WITH PLASMID
DNA
_________________________________
36
3.2.2.5
PLASMID
DNA
ISOLATION
AND
PURIFICATION
__________________________________
36
3.2.2.6
DNA
CONCENTRATION
DETERMINATION
______________________________________
37
3.2.3 BIOCHEMICAL
METHODS
_____________________________________________________
38
3.2.3.1
MAMMALIAN
CELL
LYSIS
__________________________________________________
38
3.2.3.2
MEASUREMENT
OF
PROTEIN
CONCENTRATIONS
_________________________________
38
3.2.3.3
PROTEIN
SEPARATION
USING
SDS-POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
_
38
3.2.3.4
WESTERN
BLOT
ANALYSIS
_________________________________________________
38
3.2.3.5
PROTEIN
EXPRESSION
USING
E.
COLI
__________________________________________
39
3.2.3.6
PROTEIN
AFFINITY
PURIFICATION
____________________________________________
40
3.2.3.7
QUANTIFYING
FGF2-FGFR1
INTERACTION
USING
THE
ALPHA
ASSAY
_________________
40
4
RESULTS
___________________________________________________________
42
4.1
SCREENING
A
PANEL
OF
TUMOR
CELL
LINES
FOR
AN
FGF2
VIABILITY
RESPONSE
___________
42
4.2 ESTABLISHING
A
CELL-BASED
ASSAY
TO
SCREEN
FOR
SMALL
MOLECULE
INHIBITORS
OF
FGF2
SIGNALING
________________________________________________________________
44
4.2.1
GENERATING
A
STABLE
MCHERRY-NLS
CELL
LINE
SINGLE
CLONE
FOR
AN
OPTIMAL
ASSAY
READ-OUT
_45
4.2.2
ESTABLISHING
A
KINETIC
RATIOMETRIC
ASSAY
READ-OUT
WHERE
VARYING
FGF2
CONCENTRATIONS
CAN
BE
READ-OUT
BY
MEASURING
APOPTOSIS
_______________________________________________
47
4.2.3
ESTABLISHING
AN
FGF2
SIGNALING
ASSAY
VARIANT
WHERE
FGF2
IS
SECRETED
FROM
A
TUMOR
CELL
LINE
FOR
PHYSIOLOGICAL
VALIDATION
__________________________________________________
49
4.3 SETTING
UP
THE
FGF2
SIGNALING
ASSAY
FOR
A
HIGH
THROUGHPUT
SCREEN
_____________
53
IV
4.4
SCREENING
FOR
SMALL
MOLECULE
INHIBITORS
OF
FGF2
SIGNALING,
DATA
ANALYSIS
AND
CLASSIFICATION
OF
HITS
_______________________________________________________
58
4.5
VALIDATING
AND
INITIAL
CHARACTERIZATION
OF
A
SUBSET
OF
HITS
_____________________63
4.5.1TESTING
COMPOUNDS
FOR
CONCENTRATION
DEPENDENCE
AND
POTENCY
___________________63
4.5.2
CHARACTERIZING
THE
ROLE
OF
FGF2
SIGNALING
INHIBITORS
IN
DOWNSTREAM
INHIBITION
OF
ERK
PHOSPHORYLATION
_______________________________________________________________
65
4.5.3
CHARACTERIZING
FGF2
SIGNALING
INHIBITORS
ABILITY
TO
BLOCK
THE
INTERACTION
OF
FGF2
AND
A
FRAGMENT
OF
THE
EXTRACELLULAR
DOMAIN
OF
FGFR1
_____________________________________
68
4.5.4
TESTING
THE
ABILITY
OF
THE
INHIBITORS
TO
BLOCK
FGF2
INDUCED
RESISTANCE
IN
THE
PHYSIOLOGICALLY
RELEVANT
LEUKEMIA
CELL
LINE
M0LM14
_________________________________
70
5
DISCUSSION
_________________________________________________________
75
5.1
ESTABLISHING
A
SEMI-QUANTITATIVE,
TIME-
RESOLVED
FGF2
CELL
BASED
SIGNALING
ASSAY
WITH
A
RATIOMETRIC
READ-OUT
THAT
IS
APOPTOSIS
DEPENDENT
________________________
76
5.2
OPTIMIZING
THE
FGF2
SIGNALING
SCREEN
FOR
HIGH
THROUGHPUT
MEASUREMENTS
AND
SCREENING
FOR
FGF2
SIGNALING
BLOCKERS
_______________________________________
78
5.3
VALIDATING
A
SUBSET
OF
HITS
AND
THEIR
INITIAL
CHARACTERIZATION
__________________
79
6
APPENDIX
__________________________________________________________
84
7
ACKNOWLEDGEMENTS
__________________________________________________
85
8
REFERENCES
_________________________________________________________
86
V
|
| any_adam_object | 1 |
| author2 | Li, Xiao 1944- |
| author2_role | edt |
| author2_variant | x l xl |
| author_GND | (DE-588)1020703741 |
| author_facet | Li, Xiao 1944- |
| building | Verbundindex |
| bvnumber | BV046899804 |
| classification_rvk | NK 5190 LB 57330 |
| ctrlnum | (OCoLC)1192483944 (DE-599)KXP1701720191 |
| dewey-full | 950 |
| dewey-hundreds | 900 - History & geography |
| dewey-ones | 950 - History of Asia |
| dewey-raw | 950 |
| dewey-search | 950 |
| dewey-sort | 3950 |
| dewey-tens | 950 - History of Asia |
| discipline | Geschichte Sozial-/Kulturanthropologie / Empirische Kulturwissenschaft |
| era | Geschichte gnd |
| era_facet | Geschichte |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| geographic | Seidenstraße (DE-588)4054299-3 gnd |
| geographic_facet | Seidenstraße |
| id | DE-604.BV046899804 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T08:19:29Z |
| institution | BVB |
| isbn | 9789811396434 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032309483 |
| oclc_num | 1192483944 |
| open_access_boolean | |
| owner | DE-11 DE-12 |
| owner_facet | DE-11 DE-12 |
| physical | xiii, 158 Seiten Illustrationen, Karte |
| psigel | BSB_NED_20210421 |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| record_format | marc |
| series2 | Silk Road research series |
| spellingShingle | Non-Han literature along the Silk Road |
| subject_GND | (DE-588)4054299-3 (DE-588)4143413-4 |
| title | Non-Han literature along the Silk Road |
| title_auth | Non-Han literature along the Silk Road |
| title_exact_search | Non-Han literature along the Silk Road |
| title_full | Non-Han literature along the Silk Road Xiao Li (editor) |
| title_fullStr | Non-Han literature along the Silk Road Xiao Li (editor) |
| title_full_unstemmed | Non-Han literature along the Silk Road Xiao Li (editor) |
| title_short | Non-Han literature along the Silk Road |
| title_sort | non han literature along the silk road |
| topic_facet | Seidenstraße Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032309483&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lixiao nonhanliteraturealongthesilkroad |