The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication
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| 100 | 1 | |a Tran, Vu Quynh Giao |d 1990- |e Verfasser |0 (DE-588)121197958X |4 aut | |
| 245 | 1 | 0 | |a The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |c vorgelegt von Vu Quynh Giao Tran |
| 264 | 1 | |a Münster |c 2020 | |
| 300 | |a VIII, 126 Blatt |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Westfälische Wilhelms-Universyität Münster |d 2020 |g Beschränkt für den Austausch | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-032222556 | ||
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|---|---|
| adam_text | LIST
OF
CONTENTS
LIST
OF
FIGURES
..............................................................................................................................
ILL
LIST
OF
TABLES
..............................................................................................................................
IV
ABBREVIATIONS
..............................................................................................................................
V
1.
INTRODUCTION
...........................................................................................................................
1
1.1
INFLUENZA
AND
ITS
CHARACTERISTICS
.......................................................................................
1
1.1.1
CLASSIFICATION
AND
BIOLOGY
OF
INFLUENZA
A
VIRUS
.........................................................
1
1.1.2
VIRUS
STRUCTURE
AND
PROTEINS
......................................................................................
3
1.1.3
REPLICATION
CYCLE
......................................................................................................
5
1.2
INFLUENZA
VIRUS
INDUCES
INNATE
IMMUNE
RESPONSES
IN
HOST
CELLS
.....................................
9
1.2.1
PATHOGEN
RECOGNITION
RECEPTORS
FOR
IAV
...................................................................
9
1.2.2
CYTOKINES
...............................................................................................................
11
1.2.3
POTENTIAL
FOR
IMMUNOPATHOLOGICAL
ACTION
OF
THE
INNATE
IMMUNE
RESPONSE
................
13
1.3
POST-TRANSCRIPTIONAL
REGULATION
OF
GENE
EXPRESSION
IN
INNATE
IMMUNITY
...........................
13
1.3.1
ALTERNATIVE
SPLICING
AND
POLYADENYLATION
IN
INNATE
IMMUNITY
....................................
14
1.3.2
MRNA
STABILITY
IN
INNATE
IMMUNITY
...........................................................................
15
1.3.3
TRANSLATION
EVENTS
REGULATE
THE
INNATE
IMMUNE
RESPONSE
.......................................
22
1.4
THE
RELATION
BETWEEN
THE
CELLULAR
NONSENSE-MEDIATED
MRNA
DECAY
AND
VRNAS
........
23
1.4.1
VRNAS
CAN
BE
SUBSTRATES
FOR
NMD
........................................................................
23
1.4.2
VIRUSES
HAVE
THE
CAPACITY
TO
MANIPULATE
NMD
ACTIVITY
............................................
24
1.5
AIM
OF
THIS
STUDY
............................................................................................................
25
2.
MATERIALS
AND
METHODS
.......................................................................................................
27
2.1
MATERIALS
.......................................................................................................................
27
2.1.1
COMMON
MATERIALS
.................................................................................................
27
2.1.2
MEDIA
AND
BUFFERS
..................................................................................................
36
2.1.3
HARDWARE
AND
EQUIPMENT
.......................................................................................
39
2.1.4
SOFTWARE
.................................................................................................................
40
2.2
METHODS
........................................................................................................................
40
2.2.1
CELL
CULTURE
METHODS
...............................................................................................
40
2.2.2
CRYOCONSERVATION
OF
CELLS
......................................................................................
41
2.2.3
CELL
TREATMENTS
.......................................................................................................
41
2.2.4
VIROLOGICAL
METHODS
................................................................................................
43
LIST
OF
CONTENTS
2.2.5 MOLECULAR
BIOLOGICAL
METHODS
-
NUCLEIC
ACID
........................................................
46
2.2.6
MOLECULAR
BIOLOGY
METHODS
-
PROTEINS
..................................................................
50
3.
RESULTS
................................................................................................................................
53
3.1
FUNCTIONS
OF
MRNA STABILITY
REGULATING
PROTEINS
IN
INNATE
IMMUNE
RESPONSES
DURING
INFLUENZA
A
VIRUS
INFECTIONS
......................................................................................................
53
3.1.1
SCREENINGS
FOR
EXPRESSION
PROFILES
OF
MRNA
STABILITY
REGULATING
PROTEINS
DURING
INFLUENZA
A
VIRUS
INFECTIONS
OR
PAMP
STIMULATION
(VRNA)
.....................................................
53
3.1.2
THE
DOWNSHIFT
IN
TRANSCRIPTIONAL
LEVELS
OF
NMD
PROTEINS
UPON
INFLUENZA
INFECTION
IS
MORE
PROMINENT
THAN
FOR
UNRELATED
CELLULAR
CONTROL
GENES
....................................................
58
3.1.3
PHARMACOLOGICAL
BLOCKAGE
OF
NMD
AFFECTS
INNATE
IMMUNE
RESPONSES
..................
60
3.1.4
THE
TRANSCRIPTS
OF
INNATE
IMMUNE
GENES
ARE
NOT
AFFECTED
BY
THE
SILENCING
OF
NMD
COMPONENT
EXPRESSIONS
........................................................................................................
65
3.1.5
MANIPULATING
THE
EXPRESSION
OF
UPF1
DOES
NOT
AFFECT
THE
PRO-INFLAMMATORY
RESPONSE
..............................................................................................................................
67
3.2
THE
INFLUENCE
OF
THE
NMD
PATHWAY
IN
VIRUS
REPLICATION
.................................................
73
3.2.1
SILENCING
THE
EXPRESSION
OF
UPF1
DOES
NOT
APPARENTLY
RESULT
IN
ALTERED
IAV
REPLICATION
.............................................................................................................................
74
3.2.2
KNOCKDOWN
OF
CRITICAL
NMD
PROTEINS
DOES
NOT
AFFECT
IAV
H1N1
REPLICATION
..........
78
3.2.3
KNOCKDOWN
OF
NMD
COMPONENTS
DOES
NOT
INFLUENCE
THE
REPLICATION
OF
DIFFERENT
SUBTYPES
OF
IAV
....................................................................................................................
82
3.2.4
BLOCKAGE
OF
THE
NMD
MACHINERY
BY
A
PHARMACOLOGICAL
INHIBITOR
DOES
NOT
INFLUENCE
IAV
REPLICATION
......................................................................................................................
84
3.2.5
DOWNREGULATION
OF
NMD
FACTORS
BY
RNAI
DOES
NOT
AFFECT
RESPIRATORY
SYNCYTIAL
VIRUS
(RSV)
REPLICATION
..................................................................................................................
86
4.
DISCUSSION
..........................................................................................................................
90
4.1
THE
INTERPLAY
BETWEEN
INFLUENZA
VIRUSES
AND
INNATE
IMMUNE
RESPONSES
......................
90
4.2
IAV
INFECTIONS
MANIPULATE
THE
EXPRESSION
OF
MRNA
DECAY
PROTEINS
AND
NMD
FACTORS
.92
4.3
THE
INFLUENCE
OF
THE
NMD
PATHWAY
ON
INNATE
IMMUNE
RESPONSE
AFTER
INFLUENZA
VIRUS
INFECTIONS
..................................................................................................................................
94
4.4 THE
ROLE
OF
NMD
FACTORS
IN
INFLUENZA
VIRUS
REPLICATION
...................................................
96
5.
SUMMARY
............................................................................................................................
102
6.
REFERENCES
.........................................................................................................................
103
7.
ACKNOWLEDGEMENT
............................................................................................................
122
8.
LEBENSLAUF
.........................................................................................................................
124
9.
EIDESSTATTLICHE
ERKLARUNG
................................................................................................
126
II
|
| adam_txt |
LIST
OF
CONTENTS
LIST
OF
FIGURES
.
ILL
LIST
OF
TABLES
.
IV
ABBREVIATIONS
.
V
1.
INTRODUCTION
.
1
1.1
INFLUENZA
AND
ITS
CHARACTERISTICS
.
1
1.1.1
CLASSIFICATION
AND
BIOLOGY
OF
INFLUENZA
A
VIRUS
.
1
1.1.2
VIRUS
STRUCTURE
AND
PROTEINS
.
3
1.1.3
REPLICATION
CYCLE
.
5
1.2
INFLUENZA
VIRUS
INDUCES
INNATE
IMMUNE
RESPONSES
IN
HOST
CELLS
.
9
1.2.1
PATHOGEN
RECOGNITION
RECEPTORS
FOR
IAV
.
9
1.2.2
CYTOKINES
.
11
1.2.3
POTENTIAL
FOR
IMMUNOPATHOLOGICAL
ACTION
OF
THE
INNATE
IMMUNE
RESPONSE
.
13
1.3
POST-TRANSCRIPTIONAL
REGULATION
OF
GENE
EXPRESSION
IN
INNATE
IMMUNITY
.
13
1.3.1
ALTERNATIVE
SPLICING
AND
POLYADENYLATION
IN
INNATE
IMMUNITY
.
14
1.3.2
MRNA
STABILITY
IN
INNATE
IMMUNITY
.
15
1.3.3
TRANSLATION
EVENTS
REGULATE
THE
INNATE
IMMUNE
RESPONSE
.
22
1.4
THE
RELATION
BETWEEN
THE
CELLULAR
NONSENSE-MEDIATED
MRNA
DECAY
AND
VRNAS
.
23
1.4.1
VRNAS
CAN
BE
SUBSTRATES
FOR
NMD
.
23
1.4.2
VIRUSES
HAVE
THE
CAPACITY
TO
MANIPULATE
NMD
ACTIVITY
.
24
1.5
AIM
OF
THIS
STUDY
.
25
2.
MATERIALS
AND
METHODS
.
27
2.1
MATERIALS
.
27
2.1.1
COMMON
MATERIALS
.
27
2.1.2
MEDIA
AND
BUFFERS
.
36
2.1.3
HARDWARE
AND
EQUIPMENT
.
39
2.1.4
SOFTWARE
.
40
2.2
METHODS
.
40
2.2.1
CELL
CULTURE
METHODS
.
40
2.2.2
CRYOCONSERVATION
OF
CELLS
.
41
2.2.3
CELL
TREATMENTS
.
41
2.2.4
VIROLOGICAL
METHODS
.
43
LIST
OF
CONTENTS
2.2.5 MOLECULAR
BIOLOGICAL
METHODS
-
NUCLEIC
ACID
.
46
2.2.6
MOLECULAR
BIOLOGY
METHODS
-
PROTEINS
.
50
3.
RESULTS
.
53
3.1
FUNCTIONS
OF
MRNA STABILITY
REGULATING
PROTEINS
IN
INNATE
IMMUNE
RESPONSES
DURING
INFLUENZA
A
VIRUS
INFECTIONS
.
53
3.1.1
SCREENINGS
FOR
EXPRESSION
PROFILES
OF
MRNA
STABILITY
REGULATING
PROTEINS
DURING
INFLUENZA
A
VIRUS
INFECTIONS
OR
PAMP
STIMULATION
(VRNA)
.
53
3.1.2
THE
DOWNSHIFT
IN
TRANSCRIPTIONAL
LEVELS
OF
NMD
PROTEINS
UPON
INFLUENZA
INFECTION
IS
MORE
PROMINENT
THAN
FOR
UNRELATED
CELLULAR
CONTROL
GENES
.
58
3.1.3
PHARMACOLOGICAL
BLOCKAGE
OF
NMD
AFFECTS
INNATE
IMMUNE
RESPONSES
.
60
3.1.4
THE
TRANSCRIPTS
OF
INNATE
IMMUNE
GENES
ARE
NOT
AFFECTED
BY
THE
SILENCING
OF
NMD
COMPONENT
EXPRESSIONS
.
65
3.1.5
MANIPULATING
THE
EXPRESSION
OF
UPF1
DOES
NOT
AFFECT
THE
PRO-INFLAMMATORY
RESPONSE
.
67
3.2
THE
INFLUENCE
OF
THE
NMD
PATHWAY
IN
VIRUS
REPLICATION
.
73
3.2.1
SILENCING
THE
EXPRESSION
OF
UPF1
DOES
NOT
APPARENTLY
RESULT
IN
ALTERED
IAV
REPLICATION
.
74
3.2.2
KNOCKDOWN
OF
CRITICAL
NMD
PROTEINS
DOES
NOT
AFFECT
IAV
H1N1
REPLICATION
.
78
3.2.3
KNOCKDOWN
OF
NMD
COMPONENTS
DOES
NOT
INFLUENCE
THE
REPLICATION
OF
DIFFERENT
SUBTYPES
OF
IAV
.
82
3.2.4
BLOCKAGE
OF
THE
NMD
MACHINERY
BY
A
PHARMACOLOGICAL
INHIBITOR
DOES
NOT
INFLUENCE
IAV
REPLICATION
.
84
3.2.5
DOWNREGULATION
OF
NMD
FACTORS
BY
RNAI
DOES
NOT
AFFECT
RESPIRATORY
SYNCYTIAL
VIRUS
(RSV)
REPLICATION
.
86
4.
DISCUSSION
.
90
4.1
THE
INTERPLAY
BETWEEN
INFLUENZA
VIRUSES
AND
INNATE
IMMUNE
RESPONSES
.
90
4.2
IAV
INFECTIONS
MANIPULATE
THE
EXPRESSION
OF
MRNA
DECAY
PROTEINS
AND
NMD
FACTORS
.92
4.3
THE
INFLUENCE
OF
THE
NMD
PATHWAY
ON
INNATE
IMMUNE
RESPONSE
AFTER
INFLUENZA
VIRUS
INFECTIONS
.
94
4.4 THE
ROLE
OF
NMD
FACTORS
IN
INFLUENZA
VIRUS
REPLICATION
.
96
5.
SUMMARY
.
102
6.
REFERENCES
.
103
7.
ACKNOWLEDGEMENT
.
122
8.
LEBENSLAUF
.
124
9.
EIDESSTATTLICHE
ERKLARUNG
.
126
II |
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| author | Tran, Vu Quynh Giao 1990- |
| author_GND | (DE-588)121197958X |
| author_facet | Tran, Vu Quynh Giao 1990- |
| author_role | aut |
| author_sort | Tran, Vu Quynh Giao 1990- |
| author_variant | v q g t vqg vqgt |
| building | Verbundindex |
| bvnumber | BV046814063 |
| ctrlnum | (OCoLC)1190924356 (DE-599)DNB1211744434 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046814063 |
| illustrated | Illustrated |
| index_date | 2024-07-03T14:59:43Z |
| indexdate | 2024-07-10T08:54:34Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032222556 |
| oclc_num | 1190924356 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | VIII, 126 Blatt Illustrationen 30 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| record_format | marc |
| spelling | Tran, Vu Quynh Giao 1990- Verfasser (DE-588)121197958X aut The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication vorgelegt von Vu Quynh Giao Tran Münster 2020 VIII, 126 Blatt Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische Wilhelms-Universyität Münster 2020 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1211744434/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032222556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tran, Vu Quynh Giao 1990- The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |
| subject_GND | (DE-588)4113937-9 |
| title | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |
| title_auth | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |
| title_exact_search | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |
| title_exact_search_txtP | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |
| title_full | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication vorgelegt von Vu Quynh Giao Tran |
| title_fullStr | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication vorgelegt von Vu Quynh Giao Tran |
| title_full_unstemmed | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication vorgelegt von Vu Quynh Giao Tran |
| title_short | The role of nonsense-mediated mRNA decay on host defense system and influenza virus replication |
| title_sort | the role of nonsense mediated mrna decay on host defense system and influenza virus replication |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1211744434/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032222556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT tranvuquynhgiao theroleofnonsensemediatedmrnadecayonhostdefensesystemandinfluenzavirusreplication |