Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus
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| Sprache: | English |
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2018
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| 005 | 20191108 | ||
| 007 | t | ||
| 008 | 190123s2018 gw a||| m||| 00||| eng d | ||
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| 016 | 7 | |a 1163745774 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1060620394 | ||
| 035 | |a (DE-599)DNB1163745774 | ||
| 040 | |a DE-604 |b ger |e rda | ||
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| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 |a DE-83 | ||
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| 100 | 1 | |a Mubaraka, Rafia |e Verfasser |0 (DE-588)1161955526 |4 aut | |
| 245 | 1 | 0 | |a Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus |c vorgelegt von Rafia Mubaraka aus Gujrat, Pakistan |
| 264 | 1 | |a Köln |c 2018 | |
| 300 | |a X, 130 Seiten |b Illustrationen |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität zu Köln |d 2018 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1163745774/04 |3 Inhaltsverzeichnis |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-030808114 | ||
Datensatz im Suchindex
| _version_ | 1804179302745374720 |
|---|---|
| adam_text | TABLE O F C ONTENTS
P A G E
NO.
LIST OF A BBREVIATIONS V
A BSTRACT
VII
ZUSAM M ENFASSUNG
IX
1 INTRODUCTION
1
1.1 G ROW TH RESPO NSES, PHYSIOLOGICAL AND M ETABO LIC AD A P TA TIO N S
OF P H O SP H ATE-STARVED 2
PLANTS
1.2 P H O SP H A TE STARVATION REGULATORS 3
1.2.1 P H O SP H A TE TRANSPORT
3
1 .2 .2 TRANSCRIPTION AL REGULATION OF TH E PH O SP H ATE STARVATION
RESPO NSE 4
1 .2 .3 P OST-TRANSCRIPTIONAL REGULATION OF TH E P H OSPHATE STARVATION
RESPO NSE 6
1 .2 .4 P OST-TRAN SLATIONAL REGULATION OF TH E PH O SP H ATE STARVATION
RESP O N SE
8
1 .3 M YCO RRH IZA-IN D U CED TRANSCRIPTIONAL REP RO GRAM M IN G OF
PLANT CORTICAL CELLS
11
1 .4 P H O SP H A TE REGULATION OF ARB USCULAR M YCORRHIZAL SYM BIOSIS
12
1 .5 IN TEGRATION OF P H OSPHATE STARVATION AND M YCORRHIZAL SIGNALING
PATHW AYS 13
1 .6 K EY RESEARCH QU ESTIO NS 14
2. M A TERIA L AN D M ETHOD S 16
2.1 IDENTIFICATION OF A RABIDOP SIS ORTHO LOG UES IN LOTUS JA P O N IC U
S 16
2 .1 .1 PROTEIN S EQ U E N C E S SEARCH 16
2 .1 .2 P HYTOG EN Y ANALYSIS
16
2 .1 .3 S YN TEN Y ANALYSIS 16
2 .2 P LANT LINES
17
2 .2 .1 LOTUS JA P O N IC A S
17
2 .2 .2 A RAB ID O P SIS TH ALIAN A
17
2 .3 LOTUS JA P O N IC U S SEED LING AND PLANT GROW TH CONDITIONS
17
2 .3 .1 S E E D STERILIZATION AND GERM INATION
17
2 .3 .2 M S A G A R EXP E RIM E N TS
17
2 .3 .3 SOIL/ SAN D E XP ERIM EN TS
18
2 .3 .4 P H O SP H A TE C ONTENT M E A S U RE M E N T
18
2 .3 .5 M U LTI-ELEM EN TAL ANALYTICS
18
2 .3 .6 R N A EXTRACTION
19
2 .3 .7 C D N A SYNTHESIS AND SEM IQUANTITATIVE P C R
19
2 .3 .8 Q U AN TITATIVE REAL-TIM E P C R (Q R T -P C R )
2 0
2 .3 .9 V ISUALIZATION OF ARB USCULAR M YCORRHIZAL STRUCTURES
21
2 .3 .1 0 D ETERM INATION OF COLONIZATION RATE 21
2 .4 B ACTERIA STRAINS 21
2 .4 .1 R UBIDIUM CHLORIDE CO M P E TEN T E .CO LI
21
2 .4 .2 E LECTRO -CO M P ETEN T A .RH IZO G E N E S 2 2
2 .4 .3 E SCHERICH IA COLI TRAN SFO RM ATION BY H E A T SHOCK 2 2
2 .4 .4 A G RO B ACTERIA TRANSFORM ATION BY ELECTROPORATION
22
2 .4 .5 B ACTERIAL LIQUID CULTURE AND PLASM ID PREPARATION 2 3
2 .4 .6 D N A SEQ UENCING 2 3
2 .4 .7 B ACTERIAL GLYCEROL STOCKS
2 3
2 .5 P LANT TRANSFORM ATION
2 3
2 .5 .1 T RANSFORM ATIO N OF A RABIDOP SIS THALIAN A BY FLORAL DIP
2 3
2 .5 .2 TRANSFORM ATIO N OF LOTUS JA P O N IC U S BY A GROBACTERIU M RH
IZO G EN ES
2 4
2 .5 .3 G ROW ING OF TRAN SFO RM ED LOTUS JA P O N IC U S
2 4
2 .5 .4 H ISTOCHEM ICAL ANALYSIS
2 4
2 .6 S TAN D ARD M ETH ODS
2 5
2 .6 .1 P O LYM E RAS E CHAIN REACTION (P C R )
2 5
2 .6 .2 A G A RO SE GEL ELECTRO PHORESIS
2 6
2 .7 M O LECU LAR TECH NIQ UES
2 6
2 .7 .1 C LONING WITH P G E M -T E A S Y V E C TO R S YSTEM I
2 6
2 .7 .2 C ONVENTIO NAL CLONING
2 6
2 .7 .3 G A TE W A Y CLONING
2 7
2 .8 G ROW TH M ED IA
2 7
2 .9 A NTIBIOTICS
2 8
2 .1 0 D N A LADD ERS
2 9
2.11 STATISTICAL M ETHODS
30
2 .1 1 .1 O N E W A Y A N O V A B Y S P S S
30
2 .1 1 .2 P RINCIPLE C O M P O N EN T ANALYSIS (P C A ) BY R
3 0
3. R ESULTS
31
3.1 IDENTIFICATION OF C AN D ID ATE G E N E S CODING FOR A T P H 0 2
ORTHOLOGUE IN LOTUS JA P O N IC U S
31
3 .1 .1 PROTEIN BLAST ANALYSIS
31
3 .1 .2 P HYTOG EN Y
32
3 .1 .3 R ECIP RO CAL BLAST
35
3 .1 .4 S YN TEN Y ANALYSIS
3 6
3 .1 .5 G E N E STRUCTURE
3 8
3 .1 .6 PROTEIN A LIG N M EN T OF C ONSERVED U B C DO M AIN
3 8
3 .1 .7 P H 0 2 KNOCKOUT M UTANTS
39
3 .1 .7 .1 P H O 2 -1 IS A KNOCKOUT L O R E IA IN S E RTIO N S M UTANT
4 0
3 .1 .7 .2 G ENO TYP ING O F P H O 2 -1 KNOCK-OUTS
4 0
3 .2 C HA RA C TE RIZATIO N OF L J P H 0 2 -1 LOSS O F FUNCTION M UTANT
4 2
3 .2.1 M ORPH OLO GICAL AND PHYSIOLOGICAL P H EN OTYPE
4 3
3 .2 .2 M O LECU LAR P H EN OTYPE
56
3 .2 .3 M YCO RRHIZAL P H EN OTYPE 6 3
4 . D ISCUSSION 6 5
4.1 L JP H 0 2 -1 AND L JP H 0 2 - 2 A RE TH E CLOSEST H O M O LOG UES
OF A T P H 0 2
6 5
4 .2 G E N E RA TIO N AND TESTING OF LOTUS M U TAN T LINES FOR P H O 2
-1 6 7
4 .3 L JP H 0 2 AND A T P H 0 2 H A V E OVERLAPPIN G FUNCTION REGARDING
TH E P H OSPHATE STARVATION
6 7
RESPO NSE
4 .4 L JP H 02-1 M AY FUNCTION IN M YCORRHIZAL SYM BIOSIS 7 0
5 R E FE RE N C E S
7 4
6 A P P E N D IC ES
8 9
A PPEND IX A - P RIM ERS
8 9
A PPEND IX B - PROTEIN S E Q U E N C E S FOR PHYTOGENY
92
A PPEND IX C - PROTEIN S EQ U E N C E SIM ILARITY B E TW E E N LJ P H 02
-1 AND L J P H 0 2 -2
9 8
A P P EN D IX D - O RTHO LOG OUS PROTEIN S E Q U E N C E S OF LOTUS JA P
O N IC U S IN M E D IC A G O
TRUNCATULA
9 9
ILL
A PPEND IX E - S YN TEN Y ANALYSIS
1 13
A P P EN D IX F - A LIG NM EN T OF Q R T -P C R PRODUCTS AND ITS TA RG
ET SEQ U E N C E 117
A PPEND IX G - S C RE E N E D POTENTIAL H O M O ZYG OUS L O R E IA
INSERTION LINES IN L JP H 0 2 -1
AND L JP H 0 2 - 2
1 18
A P P EN D IX H - C ONFIRM ATION OF A TP H O 2 POINT M UTATION 120
A PPEND IX I - LOTUS M ORPHOLOGICAL AND PHYSIOLOGICAL P A RAM E TE RS IN
COM PARATIVE STUDY
WITH A RABIDOP SIS
121
A P P EN D IX J - R OOT GROW TH ANALYSIS 1 23
A P P EN D IX K - P ROM OTER G U S STAINING OF L JP H 0 2 AND L JP T 4
124
A P P EN D IX L - FUNCTIONAL C OM PLEM ENTATION OF A TP H O 2 WITH A
RABIDOP SIS PROM OTER FUSED
WITH L JP H 0 2 G ENO M IC D N A
1 26
ERKLAERUNG
127
C V 128
|
| any_adam_object | 1 |
| author | Mubaraka, Rafia |
| author_GND | (DE-588)1161955526 |
| author_facet | Mubaraka, Rafia |
| author_role | aut |
| author_sort | Mubaraka, Rafia |
| author_variant | r m rm |
| building | Verbundindex |
| bvnumber | BV045422246 |
| ctrlnum | (OCoLC)1060620394 (DE-599)DNB1163745774 |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Mubaraka, Rafia Verfasser (DE-588)1161955526 aut Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus vorgelegt von Rafia Mubaraka aus Gujrat, Pakistan Köln 2018 X, 130 Seiten Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2018 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1163745774/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030808114&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mubaraka, Rafia Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus |
| subject_GND | (DE-588)4113937-9 |
| title | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus |
| title_auth | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus |
| title_exact_search | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus |
| title_full | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus vorgelegt von Rafia Mubaraka aus Gujrat, Pakistan |
| title_fullStr | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus vorgelegt von Rafia Mubaraka aus Gujrat, Pakistan |
| title_full_unstemmed | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus vorgelegt von Rafia Mubaraka aus Gujrat, Pakistan |
| title_short | Functional characterization of the E2 conjugase-like protein PHO2 in phosphate starvation and mycorrhizal symbiosis in Lotus japonicus |
| title_sort | functional characterization of the e2 conjugase like protein pho2 in phosphate starvation and mycorrhizal symbiosis in lotus japonicus |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1163745774/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030808114&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mubarakarafia functionalcharacterizationofthee2conjugaselikeproteinpho2inphosphatestarvationandmycorrhizalsymbiosisinlotusjaponicus |