G Protein Signaling Methods and Protocols
G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investi...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
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Totowa, NJ
Humana Press
2004
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| Schriftenreihe: | Methods in Molecular Biology™
237 |
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| Online-Zugang: | DE-355 DE-91 URL des Erstveröffentlichers |
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| 520 | |a G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli | ||
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| illustrated | Not Illustrated |
| indexdate | 2024-12-24T06:25:53Z |
| institution | BVB |
| isbn | 9781592594306 |
| language | English |
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| physical | 1 Online-Ressource (XII, 250 p) |
| psigel | ZDB-2-PRO |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spellingShingle | G Protein Signaling Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode (DE-588)4324907-3 gnd GTP-bindende Proteine (DE-588)4248354-2 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4324907-3 (DE-588)4248354-2 (DE-588)4318717-1 (DE-588)4143413-4 |
| title | G Protein Signaling Methods and Protocols |
| title_auth | G Protein Signaling Methods and Protocols |
| title_exact_search | G Protein Signaling Methods and Protocols |
| title_full | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka |
| title_fullStr | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka |
| title_full_unstemmed | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka |
| title_short | G Protein Signaling |
| title_sort | g protein signaling methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode (DE-588)4324907-3 gnd GTP-bindende Proteine (DE-588)4248354-2 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode GTP-bindende Proteine Signaltransduktion Aufsatzsammlung |
| url | https://doi.org/10.1385/1592594301 |
| work_keys_str_mv | AT smrckaalanv gproteinsignalingmethodsandprotocols |