The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung
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| Sprache: | English |
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August 2015
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| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV044879691 | ||
| 003 | DE-604 | ||
| 005 | 20180421 | ||
| 007 | t| | ||
| 008 | 180323s2015 gw a||| m||| 00||| eng d | ||
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| 035 | |a (DE-599)DNB1149302380 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 |a DE-860 |a DE-2174 | ||
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| 100 | 1 | |a Deppermann, Carsten |d 1985- |e Verfasser |0 (DE-588)1149302429 |4 aut | |
| 245 | 1 | 0 | |a The role of platelet granules in thrombosis, hemostasis, stroke and inflammation |b = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |c submitted by Carsten Deppermann from Frankfurt/Main, Germany |
| 246 | 1 | 1 | |a Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |
| 264 | 1 | |a Würzburg |c August 2015 | |
| 300 | |a viii, 109 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a veröffentlicht 2017 | ||
| 502 | |b Dissertation |c Julius-Maximilians-Universität Würzburg |d 2015 | ||
| 546 | |a Zusammenfassung in deutscher und englischer Sprache | ||
| 650 | 0 | 7 | |a Entzündung |0 (DE-588)4014975-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Thrombozyt |0 (DE-588)4059964-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Schlaganfall |0 (DE-588)4052588-0 |2 gnd |9 rswk-swf |
| 653 | |a Platelet granules | ||
| 653 | |a Thrombosis | ||
| 653 | |a Hemostasis | ||
| 653 | |a Stroke | ||
| 653 | |a Inflammation | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Thrombozyt |0 (DE-588)4059964-4 |D s |
| 689 | 0 | 1 | |a Entzündung |0 (DE-588)4014975-4 |D s |
| 689 | 0 | 2 | |a Schlaganfall |0 (DE-588)4052588-0 |D s |
| 689 | 0 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:20-opus-121010 |a Deppermann, Carsten, 1985- |t The role of platelet granules in thrombosis, hemostasis, stroke and inflammation |d Würzburg : Universität Würzburg, 2015 |h Online-Ressource |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1149302380/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 1 | |u https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-121010 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030273939&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-030273939 | |
Datensatz im Suchindex
| DE-BY-TUM_katkey | 2318507 |
|---|---|
| _version_ | 1820892874698391552 |
| adam_text | CONTENTS
CONTENTS III
SUMMARY VI
ZUSAMMENFASSUNG VII
1 INTRODUCTION 1
1.1
PLATELETS..................................................................................................................
1
1.2 PLATELET ACTIVATION AND THROMBUS
FORMATION............................................................. 2
1.3 PLATELET SIGNALING UPON
ACTIVATION...........................................................................
3
1.4
MEGAKARYOCYTES...................................................................................................
7
1.4.1 MEGAKARYOPOIESIS..................................... 9
1.4.2 TRANSCRIPTION FACTORS IN
MEGAKARYOPOIESIS................................................ 9
1.5 PLATELET G RA NU LE
S....................................................................................................
10
1.5.1 PLATELET GRANULE C O N TE N
T.............................................................................
10
1.5.2 PLATELET GRANULE CONTENT IN NORMAL PHYSIOLOGY AND PATHOPHYSIOLOGY
.... 11
1.5.3 BIOGENESIS OF PLATELET
GRANULES..................................................................
14
1.5.4 PLATELET
SECRETION........................................................................................
15
1.5.5 PLATELET GRANULE
DISORDERS..........................................................................
17
1.6 AIM OF THIS S TU D Y
....................................................................................................
16
2 MATERIALS AND METHODS 20
2.1 MATERIALS
...............................................................................................................
20
2.1.1 CHEMICALS AND
REAGENTS.............................................................................
20
2.1.2 ANTIBODIES
.
...................................................................................................
21
2.1.3 BUFFERS
......................................................................................................
22
2.1.4 A NIM
ALS......................................................................................................
25
2.2
METHODS..................................................................................................................
26
2.2.1 GENOTYPING OF MICE
...................................................................................
26
2.2.2 MOLECULAR BIOLOGY AND BIOCHEMISTRY . . .
.
.......................................... 28
2.2.3
IN VITRO
ANALYSES OF PLATELET FUNCTION
......................................................... 29
2.2.4
IN VIVO
ANALYSES OF PLATELET
FUNCTION............................................................ 32
2.2.5 MK A N A LYSE
S..............................................................................................
34
2.2.6 ELECTRON
MICROSCOPY..................................................................................
35
2.2.7 H ISTOLO G
Y....................................................................................................
36
2.2.8 STATISTICAL DATA A N A LY S IS
..............................................................................
37
3 RESULTS 38
3.1 ANALYSIS OF MUND 3-4-DEFICIENT M IC E
..................................................................... 38
3.1.1
UNC13CT/
MICE DISPLAY UNALTERED PLATELET COUNTS AND GRANULE NUMBERS . . 38
3.1.2 ABROGATED ATP SECRETION AND AGGREGATION OF
U N D 3DA
P LA TE LE TS ............ 38
3.1.3
UNC13DA
PLATELETS SHOW IMPAIRED P-SELECTIN EXPOSURE AND INTEGRIN ACTI
VATION AFTER STIMULATION AND ADHESION TO COLLAGEN UNDER FLO W
....................
39
3.1.4 DEFECTIVE
IN VIVO
THROMBUS FORMATION AND HEMOSTASIS IN
UNC13CTA
MICE . 41
3.1.5 REDUCED INFARCT SIZE AND NO SIGNS OF HEMORRHAGE IN MUND
3-4-DEFICIENT
MICE AFTER TM C A O
......................................................................................
42
3.2 ANALYSIS OF NBEAL2-DEFICIENT M ICE
...........................................................................
46
3.2.1
NBEAL 2A
MICE ARE MACROTHROMBOCYTOPENIC AND LACK -GRANULES IN MKS
AND P LA TE LE TS
...............................................................................................
46
3.2.2 DEFECTIVE ADHESION AND AGGREGATE FORMATION OF
NBEAL2A
PLATELETS UNDER
F L O W
.............................................................................................................
55
3.2.3 SEVERELY DEFECTIVE ARTERIAL THROMBUS FORMATION AND HEMOSTASIS IN
NBEAL2-
DEFICIENT M
ICE...............................................................................................
57
3.2.4
NBEAL2A
MICE ARE PROTECTED IN A MODEL OF ISCHEMIC STROKE
.......................
60
3.2.5
NBEAL2A
MICE SHOW IMPAIRED DERMAL HEALING DUE TO REDUCED TGF-SS RE
LEASE FROM MUTANT P LA TE LE
TS........................................................................
61
3.2.6 COMPARISON OF
NBEAL2A
AND HUMAN GPS PLATELETS AND M K S
.................
62
3.3 ANALYSIS OF MUND 3-4/NBEAL2-DEFICIENT M ICE
.........................................................
65
3.3.1 MACROTHROMBOCYTOPENIA BUT UNALTERED PLATELET GLYCOPROTEIN
EXPRESSION IN
UNC13CTA/NBEAL2A
M ICE
..............................................................................
65
3.3.2 ABROGATED P-SELECTIN EXPOSURE AND REDUCED INTEGRIN ACTIVATION UPON
STIM
ULATION OF MUND 3-4/NBEAL2-DEFICIENT PLATELETS
........................................
65
3.3.3
UNC13CTA/NBEAL2~A
PLATELETS SHOW DEFECTIVE AGGREGATION, ADHESION TO COL
LAGEN UNDER FLOW AND PS EXPOSURE
............................................................
67
3.3.4 MUND 3-4/NBEAL2-DEFICIENT MICE DISPLAY IMPAIRED THROMBOSIS AND
HEMOSTA
SIS
IN V IV O
....................................................................................................
69
3.3.5
U ND 3CTA/NBEAL2A
MICE MAINTAIN VASCULAR INTEGRITY AT SITES OF LOCAL IN
FLAMMATION BUT NOT ISCHEMIC STRO KE
............................................................
69
4 DISCUSSION 73
4.1 PLATELET DENSE GRANULES IN THROMBOSIS, HEMOSTASIS AND
STROKE............................. 73
4.2 PLATELET -GRANULES IN THROMBOSIS, HEMOSTASIS AND S TR O K E
..................................
74
4.2.1 THE
NBEAL2A
MOUSE AS A MODEL FOR THE GRAY PLATELET SYNDROM E
..............
77
4.2.2 THE ROLE OF NBEAL2 IN -GRANULE BIOGENESIS
...........................................
78
4.3 COMBINED DEFICIENCY OF PLATELET- AND DENSE GRANULE
SECRETION............
..............
79
4.4 CONCLUDING REMARKS AND O U TLO O K
...........................................................................
82
BIBLIOGRAPHY 84
5 APPENDIX 101
5.1
ABBREVIATIONS.............................................................................................................101
5.2
ACKNOWLEDGEMENTS....................................................................................................
104
5.3 CURRICULUM V IT A E
.......................................................................................................105
5.4
PUBLICATIONS...............................................................................................................
107
5.4.1 RESEARCH ARTICLES
...........................................................................................
107
5.4.2 SELECTED TALKS AND
PRESENTATIONS..................................................................107
5.5 A FFID A
VIT.....................................................................................................................
109
5.6 EIDESSTATTLICHE ERKLAERUNG
.........................................................................................109
|
| any_adam_object | 1 |
| author | Deppermann, Carsten 1985- |
| author_GND | (DE-588)1149302429 |
| author_facet | Deppermann, Carsten 1985- |
| author_role | aut |
| author_sort | Deppermann, Carsten 1985- |
| author_variant | c d cd |
| building | Verbundindex |
| bvnumber | BV044879691 |
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| ctrlnum | (OCoLC)1136258064 (DE-599)DNB1149302380 |
| discipline | Medizin |
| format | Thesis Book |
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| id | DE-604.BV044879691 |
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| indexdate | 2024-12-24T06:23:16Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030273939 |
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| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-860 DE-2174 |
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| physical | viii, 109 Seiten Illustrationen, Diagramme 30 cm |
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| spellingShingle | Deppermann, Carsten 1985- The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung Entzündung (DE-588)4014975-4 gnd Thrombozyt (DE-588)4059964-4 gnd Schlaganfall (DE-588)4052588-0 gnd |
| subject_GND | (DE-588)4014975-4 (DE-588)4059964-4 (DE-588)4052588-0 (DE-588)4113937-9 |
| title | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |
| title_alt | Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |
| title_auth | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |
| title_exact_search | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |
| title_full | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung submitted by Carsten Deppermann from Frankfurt/Main, Germany |
| title_fullStr | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung submitted by Carsten Deppermann from Frankfurt/Main, Germany |
| title_full_unstemmed | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung submitted by Carsten Deppermann from Frankfurt/Main, Germany |
| title_short | The role of platelet granules in thrombosis, hemostasis, stroke and inflammation |
| title_sort | the role of platelet granules in thrombosis hemostasis stroke and inflammation zur rolle der thrombozytengranula in thrombose hamostase schlaganfall und entzundung |
| title_sub | = Zur Rolle der Thrombozytengranula in Thrombose, Hämostase, Schlaganfall und Entzündung |
| topic | Entzündung (DE-588)4014975-4 gnd Thrombozyt (DE-588)4059964-4 gnd Schlaganfall (DE-588)4052588-0 gnd |
| topic_facet | Entzündung Thrombozyt Schlaganfall Hochschulschrift |
| url | http://d-nb.info/1149302380/04 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-121010 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030273939&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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