Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme
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Oktober 2016
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| 100 | 1 | |a Hegde, Vishal |d 1987- |e Verfasser |0 (DE-588)1148147772 |4 aut | |
| 245 | 1 | 0 | |a Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme |c vorgelegt von Vishal Hegde aus Manipal, Indien |
| 264 | 1 | |a Köln |c Oktober 2016 | |
| 300 | |a 2 Mikrofiche (I, 101 Seiten) |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b h |2 rdamedia | ||
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| 340 | |f 24x | ||
| 502 | |b Dissertation |c Universität Köln |d 2016 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-030264439 | |
Datensatz im Suchindex
| _version_ | 1819785537303609344 |
|---|---|
| adam_text | TABLE OF CONTENTS
ABSTRACT....................................................................................................................................................................................1
ZUSA M M E N FA S SU N G
............................................................................................................................................................
3
1. IN T R O D U C T IO N
.............................................................................................................................................................5
1 .1 . I
M P O R T A N C E
OF
D E G R A D A TIO N
IN
M A IN T A IN IN G
P RO TEIN
A N D
CELLULAR
H O M E O S T A S IS
...................................
5
1 .2 . L
YS O S O M A L
D E G R A D A T IO N
S Y S T E M
................................................................................................................................
5
1 .3 . U
B IQ U IT IN
-P
R O TE A S O M E
S Y S T E M
...................................................................................................................................6
1 .4 . T
H E
2 6 S
P R O T E A S O M E
.......................................................................................................................................................
6
1 .5 . U
B IQ U IT IN
-
IN D E P E N D E N T
D E G R A D A T IO N
.......................................................................................................................8
1 .6 . U
B IQ U IT IN
-
D E P E N D E N T
D E G R A D A T IO N
...........................................................................................................................9
1 .7 . P
O L Y A M IN E S
......................................................................................................................................................................
1 4
1 .8 . O
R N IT H IN E
D
E C A R B O X Y LA S E
..........................................................................................................................................2
1
1 .9 . A
N T IZ Y M E
...........................................................................................................................................................................
22
1 .1 0 .
DMAI
AND
DMA2
........................................................................................................................................................
2 6
2. A IM OF THE CURRENT S T U D Y
...............................................................................................................................28
3. MATERIALS A ND M E T H O D S
...................................................................................................................................29
3 .1 . M
A T E R IA L S
........................................................................................................................................................................
2 9
3 .2 . M
E T H O D S
...........................................................................................................................................................................
3 8
3 .2 .1 . M
OLECULAR
BIO LO G Y
T E C H N IQ U E S
...............................................................................................................................3
8
3 .2 .2 . B
IO C H E M IC A L
A N D
IM M U N O L O G IC A L
M E T H O D S
........................................................................................................4
2
4. RESULTS
.......................
47
4 .1 . U
B IQ U IT IN
LIGASES
FOR
O
A Z
I
A N D
TH EIR
R E G U L A T IO N
...........................................................................................
4 7
4 .1 .1 .
DMA1
AND
DMA2
ARE UBIQUITIN LIGASES MEDIATING A PROTEOLYTIC CONTROL OF O A Z L
........................
4 7
4 .1 .2 . P
O L Y A M IN E S
IN H IB IT
U B IQ U IT Y L A T IO N
OF
O
A Z
I
IN
V I V O
.........................................................................................5
0
4 .1 .3 . D
M A
I
A N D
D
M A
2
B IN D
O
A Z
I
IN
V IT R O
.....................................................................................................................
51
4 .1 .4 . P
O L Y A M IN E S
IN H IB IT
B IN D IN G
O F
D
M A
2
T O
O
A Z
I
IN
V IT R O
....................................................................................52
4 .1 .5 . FHA DOMAIN OF DMA2 IS THE MAIN O A Z L INTERACTING
MOTIF..........................................................................
53
4 .1 .6 .
N
TERMINUS DOMAINS OF DMA2 HAVE REGULATORY ROLES IN TARGETING O A Z L
............................................
5 6
4 .1 .7 . O
A Z
I
S T IM U L A T E S
A U T O
-
U B IQ U IT Y L A T IO N
O F
D
M A
2
IN
V IT R O
..............................................................................
5 9
4 .1 .8 . F
L U C T U A T IO N S
OF
O
A Z
I
LEVELS
D U R IN G
CELL
D IV IS IO N
CYCLE
IS
D E P E N D E N T
O N
D
M A
2
.................................
6 1
4 .1 .9 . DM A DAMAGE INDUCED O A Z L DEGRADATION DEPENDS ON
DMAI
AND
DMA2
............................................
6 4
4 .1 .1 0 . D
M A
2
IS
ALSO
REGULATED
IN
A
S IM ILA R
CELL
CYCLE
-
D E P E N D E N T
PATTERN
AS
O
A Z
I
........................................
6 5
4 .2 .1 . A UCC SERINE CODON IN THE RIBOSOMAL FRAMESHIFT SITE OF A Z1 M
R N A IS INCOMPATIBLE WITH DECODING
IN
YEAST......................................................................................................................................................................................6
7
4 .2 .2 . POLYAMINE-DEPENDENT FRAMESHIFTING IS ABSENT IN A Z1 EXPRESSED
IN S.CEREVISIAE
........................................
6 8
4 .2 .3 .
AZ1
FRAMESHIFTING IS ALSO DEPENDENT UPON PRESENCE OF A HUNGRY CODON AT THE
PENULTIMATE
POSITION PRECEDING THE O R F1 STOP
CODON.................................................................................................................
6 9
4 .2 .4 . T
RANSFER
OF
TH E
3
EN D
OF
OAZI
O N T O
AZ1
IS
N O T
SU FFIC IE N T
TO
IN FLIC T
P O L Y A M IN E
-
R E G U LA TIO N
O N
ITS
DECODING.................................................................................................................................................................................7
0
4 .2 .5 . 5 END OF
AZ1
IS INHIBITORY IN ITS
FUNCTION...........................................................................................................7
2
4 .2 .6 . T
HE
N -
T E R M IN A L
N A SC EN T
POLYPEPTIDE
M IG H T
BE
IN V O L V E D
IN
P O L Y A M IN E
-
D E P E N D E N T
RIBOSOMAL
FRAMESHIFTING OF A Z 1
.........................................................................................................................................................7
4
4 .2 .7 . H
U M A N
A
N T IZ Y M E
IS
TA R G ETED
IN
YEAST
VIA
A
DMA1-
A N D
D M A 2 -
M E D IA T E D
,
U B IQ U IT IN
D E P E N D E N T
DEGRADATION
PATHWAY.......................................................................................................................................................
75
5.
DISCUSSION...................................................................................................................................................................77
5 .1 . D M A 1
A N D
D M A 2
ARE
B O N A
FIDE
U B IQ U IT IN
LIGASES
FOR
S
A C C H A R O M YC ES
CEREVISIAE
A N T IZ Y M E
.........
7 7
5 .2 . P
O L Y A M IN E
IN H IB IT S
TH E
D E G R A D A T IO N
O F
O
A Z
I
....................................................................................................79
5 .3 . T
HE
U B IQ U IT IN
LIGASES
REGULATE
TH E IR
O W N
LEVELS
TH R O U G H
A U T O
-
U B IQ U IT Y L A T IO N
................................
8 0
5 .4 . A
U T O
-
IN H IB IT IO N
OF
TH E
FHA
D O M A IN
OF
D
M A
2
V IA
ITS
N -
T E R M IN U S
.............................................................8 0
5 .5 . C
ELL
CYCLE
-
A N D
D N A
D A M A G E
-
IN D U C E D
R EG U LA TIO N
O F
O
A Z
I
IS
D M A 1 -
A N D
D M A 2 -
D E P E N D E N T
... 82
5 .6 . R
E C O G N IT IO N
O F
TH E
P E N U L T IM A T E
C O D O N
BEFORE
TH E
F R A M E S H IFT
SITE
BY
A
N EAR
-
C O G N A TE
PEPTIDYL
-
T
R NA
IS IMPORTANT FOR FRAMESHIFTING EFFICIENCY OF HUMAN ANTIZYME IN YEAST
.......................................................
8 4
5 .7 . RIBOSOMAL FRAMESHIFTING OF HUMAN ANTIZYME MIGHT INVOLVE
CIS-ACTING FACTORS..............................8 6
5 .8 . T
HE
C-
T E R M IN U S
OF O
A Z
I IS UNABLE TO IMPOSE POLYAMINE-DEPENDENT FRAMESHIFTING WHEN REPLACING
THE C-TERMINUS OF A Z L
.......................................................................................................................................................
8 7
REFERENCES..............................................................................................................................................................................
89
ABBREVIATIONS.......................................................................................................................................................................98
ACKNOWLEDGEM EN
T...........................................................................................................................................................99
EIDESSTATTLICHE
ERKLAERUNG.......................................................................................................................................
100
LEBENSLAUF
101
|
| any_adam_object | 1 |
| author | Hegde, Vishal 1987- |
| author_GND | (DE-588)1148147772 |
| author_facet | Hegde, Vishal 1987- |
| author_role | aut |
| author_sort | Hegde, Vishal 1987- |
| author_variant | v h vh |
| building | Verbundindex |
| bvnumber | BV044869974 |
| ctrlnum | (OCoLC)1135409079 (DE-599)DNB1150261323 |
| discipline | Biologie |
| format | Thesis Microfilm Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
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| illustrated | Illustrated |
| indexdate | 2024-12-24T06:22:58Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030264439 |
| oclc_num | 1135409079 |
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| physical | 2 Mikrofiche (I, 101 Seiten) Illustrationen |
| publishDate | 2016 |
| publishDateSearch | 2016 |
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| spellingShingle | Hegde, Vishal 1987- Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme |
| subject_GND | (DE-588)4113937-9 |
| title | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme |
| title_auth | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme |
| title_exact_search | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme |
| title_full | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme vorgelegt von Vishal Hegde aus Manipal, Indien |
| title_fullStr | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme vorgelegt von Vishal Hegde aus Manipal, Indien |
| title_full_unstemmed | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme vorgelegt von Vishal Hegde aus Manipal, Indien |
| title_short | Regulation of polyamine biosynthesis: proteolytic control of Ornithine Decarboxylase-Antizyme |
| title_sort | regulation of polyamine biosynthesis proteolytic control of ornithine decarboxylase antizyme |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1150261323/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030264439&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hegdevishal regulationofpolyaminebiosynthesisproteolyticcontrolofornithinedecarboxylaseantizyme |