Plant argonaute proteins methods and protocols
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| Format: | Buch |
| Sprache: | English |
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New York, NY
Humana Press
[2017]
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| Schriftenreihe: | Methods in molecular biology
1640 |
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Datensatz im Suchindex
| _version_ | 1819673913786892288 |
|---|---|
| adam_text | Titel: Plant Argonaute proteins
Autor: Carbonell, Alberto
Jahr: 2017
Contents
Preface.......................................................... i?
Contributors...................................................... ix
1 Plant ARGONAUTEs: Features, Functions, and Unknowns.............. 1
Alberto Carbonell
2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs
and 5 Cleavage Products of Their Target RNAs in Plants................ 23
Guodong Ren, Xiaoyan Wang, and Bin Yu
3 In Vitro Formation of Plant RNA-Induced Silencing Complexes
Using an Extract of Evacuolated Tobacco Protoplasts................... 39
Taichiro Iki, Masayuki Ishikawa, and Manabu Toshikawa
4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage
and Translational Repression in Plants............................... 55
Yukihide Tomari and Hiro-oki Iwakawa
5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants...... 73
Kdroly Fdtyol and Jozsef Burgydn
6 Immunoprecipitation and High-Throughput Sequencing
of ARGONAUTE-Bound Target RNAs from Plants.................... 93
Alberto Carbonell
7 Identification of ARGONAUTE/Small RNA Cleavage Sites
by Degradome Sequencing....................................... 113
Ivett Baksa and Gydrgy Szittya
8 Dissecting the Subnuclear Localization Patterns of Argonaute
Proteins and Other Components of the RNA-Directed DNA
Methylation Pathway in Plants.................................... 129
Cheng-Guo Duan and Jian-Kang Zhu
9 Detection of Argonaute 1 Association with Polysomes
in Arabidopsis thaliana.......................................... 137
Cecile Lecampion, Elodie Lanet, and Christophe Robaglia
10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis
and DNA Repair............................................... 145
Marina Martinez-Garcia and Monica Pradillo
11 Isolation and Characterization of ARGONA TJTE Mutants
in Chlamydomonas............................................. 159
Tomohito Yamasaki
12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification,
Characterization, and Genetic Transformation......................... 173
Meizhen Wang, Taxing Deng, Eenjuan Shao, Miaomiao Liu,
Yongqi Pang, Caili Li, and Shanfa Lu
vii
viii
Contents
13 Cloning and Characterization of Argonauts Genes in Tomato............. 191
Zbiqiang Xian, Fang Tan, and Zhengguo Li
14 Nonradioactive Detection of Small RNAs Using
Digoxigenin-Labeled Probes...................................... 199
Ariel H. Tomassi, Delfina Gqgliardi, Damian A. Cambiagno,
and Pablo A. Manavella
15 Nonradioactive Plant Small RNA Detection Using
Biotin-Labeled Probes........................................... 211
Jun Flu and Tingguo Zhu
16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent
DNA Methylation in Plants....................................... 219
Kai Tang, Cheng-Guo Duan, Huiming Zhang, and Jian-Kang Zhu
17 Structural and Functional Characterization of Plant ARGONAUTE
MID Domains................................................ 227
Filipp Frank and Bhushan Nagar
18 Identification and Analysis of WG/GW
ARGONAUTE-Binding Domains.................................. 241
Andrzej Zielezinski and Wojciech M. Karlowski
19 Identification and Evolutionary Characterization
of ARGONAUTE-Binding Platforms............................... 257
Joshua T. Trujillo and Rebecca A. Mosher
20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES.......... 267
Ravi K. Singh and Shree P. Pandey
Index............................................................... 295
|
| any_adam_object | 1 |
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| dewey-full | 580 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 580 - Plants |
| dewey-raw | 580 |
| dewey-search | 580 |
| dewey-sort | 3580 |
| dewey-tens | 580 - Plants |
| discipline | Biologie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-12-24T05:58:35Z |
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| isbn | 9781493971640 1493971646 |
| language | English |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer Protocols |
| spellingShingle | Plant argonaute proteins methods and protocols Methods in molecular biology |
| title | Plant argonaute proteins methods and protocols |
| title_auth | Plant argonaute proteins methods and protocols |
| title_exact_search | Plant argonaute proteins methods and protocols |
| title_full | Plant argonaute proteins methods and protocols |
| title_fullStr | Plant argonaute proteins methods and protocols |
| title_full_unstemmed | Plant argonaute proteins methods and protocols |
| title_short | Plant argonaute proteins |
| title_sort | plant argonaute proteins methods and protocols |
| title_sub | methods and protocols |
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