Lentiviral vectors and exosomes as gene and protein delivery tools

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Weitere Verfasser: Federico, Maurizio (HerausgeberIn)
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Sprache:English
Veröffentlicht: New York Humana Press [2016]
Schriftenreihe:Methods in molecular biology 1448
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adam_text Titel: Lentiviral vectors and exosomes as gene and protein delivery tools Autor: Federico, Maurizio Jahr: 2016 Contents Preface.......................................................... v Contributors...................................................... ix Part I Recent Improvements in LV Construction, Production, and Transduction 1 Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown.................................. 3 Angeline de Bruyns, Ben Gelling, and David Dankort 2 Development of Inducible Molecular Switches Based on All-in-One Lentiviral Vectors Equipped with Drug Controlled FLP Recombinase....... 23 Tobias Maetzig and Axel Schambach 3 Production of Retrovirus-Based Vectors in Mildly Acidic pH Conditions .... 41 Nathalie Holic and David Fenard 4 Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G........... 49 NataSa Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt, and Christian Thirion Part II New LV Targets and Applications 5 Transduction of Murine Hematopoietic Stem Cells with Tetracycline-regulated Lentiviral Vectors......................... 65 Maike Stahlhut, Axel Schambach, and Olga S. Kustikova 6 Introduction of shRNAs, miRNAs, or AntagomiRs into Primary Human Liver Cells Through Lentiviral Vectors........................ 77 Jessica K. Rieger and Maria Thomas 7 Production and Concentration of Lentivirus for Transduction of Primary Human T Cells....................................... 85 Alan Kennedy and Adam P. Cribbs 8 Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer........................................... 95 Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, and Robert Winston 9 The LAM-PCR Method to Sequence LV Integration Sites............... 107 Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, and Manfred Schmidt vii viii Contents 10 Conditional RNAi Using the Lentiviral GLTR System................... 121 Elisabeth Pfeijfenberger, Reinhard Sigl, and Stephan Geley 11 Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies............................. 139 Aur6lien Lathuiliire and Bernard L. Schneider Part III Integrase-Mutant LVs 12 Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector-Transduced. 293T Cell Line........ 159 Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, and M. K. Azaham A. Hamid 13 Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson s Disease............................... 175 Njjoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. YAnez-Munoz 14 Development of Lentiviral Vectors for Targeted Integration and Protein Delivery............................................ 185 Diana Schenkwein and Seppo Yld-Herttuala Part IV Production, Detection, and Engineering of Exosomes 15 Biogenesis and Functions of Exosomes and Extracellular Vesicles........... 201 Florian Dreyer and Andreas Baur 16 Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes.................................... 217 Carolina Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana Zanetti, Maria Luisa Fiani, and Massimo Sargiacomo 17 Cardiac Myocyte Exosome Isolation................................ 237 ZulfiqarA. Malik, Tingting T. Liu, and Anne A. Knowlton 18 Incorporation of Heterologous Proteins in Engineered Exosomes.......... 249 Francesco Manfredi, Paola Di Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico 19 Exosome-Mediated Targeted Delivery of miRNAs...................... 261 Shin-ichiro Ohno and Masahiko Kuroda Index........................................................... 271
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Springer protocols
spelling Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
New York Humana Press [2016]
2016
xi, 276 Seiten Illustrationen
txt rdacontent
n rdamedia
nc rdacarrier
Methods in molecular biology 1448
Springer protocols
LentivirusesvLaboratory manuals
Lentivirus
Laboratory Manuals
Federico, Maurizio edt
Erscheint auch als Online-Ausgabe 978-1-4939-3753-0
Methods in molecular biology 1448 (DE-604)BV035362695 1448
HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029168831&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle Lentiviral vectors and exosomes as gene and protein delivery tools
Methods in molecular biology
LentivirusesvLaboratory manuals
Lentivirus
title Lentiviral vectors and exosomes as gene and protein delivery tools
title_auth Lentiviral vectors and exosomes as gene and protein delivery tools
title_exact_search Lentiviral vectors and exosomes as gene and protein delivery tools
title_full Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
title_fullStr Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
title_full_unstemmed Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
title_short Lentiviral vectors and exosomes as gene and protein delivery tools
title_sort lentiviral vectors and exosomes as gene and protein delivery tools
topic LentivirusesvLaboratory manuals
Lentivirus
topic_facet LentivirusesvLaboratory manuals
Lentivirus
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029168831&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link (DE-604)BV035362695
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