Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Düsseldorf
VDI Verlag GmbH
[2016]
|
| Ausgabe: | Als Manuskript gedruckt |
| Schriftenreihe: | Fortschritt-Berichte VDI. Reihe 5, Grund- und Werkstoffe, Kunststoffe
Nr. 760 |
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| 100 | 1 | |a Derr, Ludmilla |d 1984- |e Verfasser |0 (DE-588)1107057949 |4 aut | |
| 245 | 1 | 0 | |a Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity |c Dipl.-Chem. Ludmilla Derr, Bremen |
| 246 | 1 | 3 | |a Interactions: enzymes and colloidal particles |
| 250 | |a Als Manuskript gedruckt | ||
| 264 | 1 | |a Düsseldorf |b VDI Verlag GmbH |c [2016] | |
| 264 | 4 | |c © 2016 | |
| 300 | |a XIX, 189 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Fortschritt-Berichte VDI. Reihe 5, Grund- und Werkstoffe, Kunststoffe |v Nr. 760 | |
| 502 | |b Dissertation |c Universität Bremen |d 2016 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-029132493 | |
Datensatz im Suchindex
| DE-BY-TUM_call_number | 0701 65 B 276 |
|---|---|
| DE-BY-TUM_katkey | 2227460 |
| DE-BY-TUM_location | Mag |
| DE-BY-TUM_media_number | 040071446334 |
| _version_ | 1820895909565693952 |
| adam_text | CONTENTS
ACKNOWLEDGMENTS
III
CONTENTS
V
UST OF ABBREVIATIONS AND SYMBOLS XI
SUMMARY
XVI
ZUSAMMENFASSUNG
XVIII
1
1
INTRODUCTION
I
1.1
AIMS AND MOTIVATION
5
N AQUEOUS SUSPENSION
* 2
INTERACTIONS BETWEEN COLLOIDAL PARTICLES
5
2.1
VAN DER WAALS FORCES
8
2.2
ELECTROSTATIC DOUBLE LAYER FO
2.2.1
SURFACE CHARGE *
I I
2.2.2
ZETA POTENTIAL
13
2.2.3
THE POISSON-BOLTZMANN EQUATION
17
2.2.4
THE ELECTROSTATIC NTERACTJON ENERGY BETWEEN TWO SPHERES
18
2.3
THE DLVO THEORY
20
2.4
NON-DLVO FORCES
22
3
PROTEINS
22
^3.1
THE AMINO ACID COMPOSITION OF P
**
E
23
3.2
PROTEIN STRUCTURE
24
3.3
ENZYMES
25
3.3.1
-
.
CHYMOTRYPSIN
ON AND COVALENT ENZYME
*
SURFACE FUNCTJ
*
NALJZAT
*
*4
COLOIDAL PARTICLES, THE
27
MMOBILIZATLON
27
4.1
ALUMINA COLLOIDAL PARTICLES
4.2 SILICA COLLOIDAL PARTICLES 28
4.3 TITANLA COLLOIDAL PARTICLES 29
4.4 SURFACE FUNCTIONALIZATION OF COLLOIDAL PARTICLES 30
4.5 COVALENT ENZYME *MMOBJLIZATJON 31
5 PROTEIN ADSORPTION 34
5.1 INFLUENCES ON PROTEIN-PARTICLE INTERACTIONS 34
5.2 THERMODYNAMIC APPROACH 36
5.3 PROTEIN ADSORPTION MODELS 37
5.3.1 THE LANGMUIR ADSORPTION MODEL 37
5.3.2 OTHER ADSORPTION MODELS 39
5.4 METHODS FOR STUDYING PROTEIN-PARTICLE ADSORPTION 39
6 ENZYMATIC ACTIVITY 41
6.1 MICHAELIS-MENTEN KINETICS 41
6.2 DETERMINARON OF
**
AND
VMAX
FROM LINEARIZATION PLOTS 45
6.3 ACTIVITY ASSAYS: PRINCIPLES 46
7 EXPERIMENTAL METHODS AND PRINCIPLES 48
7.1 ELECTROACOUSTIC SPECTROSCOPY: ZETA POTENTIAL 48
7.2 GAS ADSORPTJON ISOTHERMS: SPECIFIC SURFACE AREA SSA
BET
,
HYDROPHOBICITY/ HYDROPHILICITY 50
7.3 UV-V*S SPECTROSCOPY: PROTEIN QUANTIFICATION 51
7.4 ACTIVITY ASSESSMENT OF FREE AND ADSORBED A-CHYMOT
*
YPS*N 53
7.5 MATRIX-ASSISTED LASER DESORPTION *ONIZATJON TIME*OF*FLIGHT MASS
SPECTROSCOPY 55
7.6 CIRCULAR DICHROISM SPECTROSCOPY: CHANGES
***
PROTEIN STRUCTURE 56
7.7 VISUAL MOLECULAR DYNAMICS: MODELING OF PROTEINS 58
8 PHYSISORPTION OF
ENZYMATICALLY ACTIVE CHYMOTRYPSIN ON
TITANIA
COLLOIDAL
PARTJDES
59
8.1 INTRODUCTION
59
8.2 EXPERIMENTAL SECTION 61
8.2.1 MATERIALS 61
8.2.2 CHARACTERIZATION OFT
*
2
COLLOIDAL PARTICLES 61
8.2.3 HYDROPHOBIC/ HYDROPHILIC PROPERTIES OF CHYMOTRYPSIN 62
8.2.4 TIME-DEPENDENT AND PH-DEPENDENT ADSORPTION/ DESORPTION OF
CHYMOTRYPSIN 63
8.2.5 CONCENTRATION-DEPENDENT ADSORPTION OF CHYMOTRYPSIN ON *102 65
8.2.6 DETERMINATION OF THE ENZYMATJC ACTIVITY OF CHYMOTRYPSJN 65
8.2.7 STATISTICAL ANALYSIS 67
8.3 RESULTS 68
8.3.1 CHARACTERIZATION OF TJ
*
2
COLLOIDAL PARTICLES 68
8.3.2
TME-DEPENDENT AND PH-DEPENDENT ADSORPTION/ DESORPTION OF
CHYMOTRYPSIN 70
8.3.3 CONCENTRAT*ON-DEPENDENT ADSORPTION OF CHYMOTRYPSIN ON TI
*
2
71
8.3.4 SUBSTRATE CONCENTRATION-DEPENDENCY FOR FREE AND ADSORBED
CHYMOTRYPSIN 73
8.4 DISCUSSION 75
8.5 CONCLUSIONS 79
8.6 ACKNOWLEDGMENTS 80
8.7 SOURCE 81
9 EFFECTS OF SIL*CA AND ALUMJNA COLLOIDAL PARTICLES ON THE ENZYMATIC
ACTIVITY
OF A-CHYMOTRYPS*N AFTER ADSORPTION 82
9.1 INTRODUCTION 82
9.2 EXPERIMENTAL SECTION 83
9.2.1 MATERIALS 83
9.2.2 CHARACTERIZATION OF SI
*
2
AND **
2 0 3
84
9.2.3 SIZE
DETERMINATION AND HYDROPHOBIC/ HYDROPHILIC PROPERTIES OF
CHYMOTRYPSIN
85
9.2.4 ADSORPTION AND DESORPTJON STUDIES OF CHYMOTRYPSJN 86
9.2.5 QUANTITATIVE DETERMINATION OF ADSORBED CHYMOTRYPSIN 88
9.2.6 ENZYMATIC
ACTIVITY MEASUREMENTS OF FREE CHYMOTRYPSIN 88
9.2.7 ENZYMATJC ACTIVITY MEASUREMENTS OF ADSORBED CHYMOTRYPSIN 88
9.2.8 STATISTICAL ANALYSIS 89
9.3 RESULTS 90
9.3.1 CHARACTERIZATION OF SI
*
2
AND **
2 0 3
90
9.3.2 SIZE
DETERMINATION AND HYDROPHOBIC/ HYDROPHILIC PROPERTIES
OF
CHYMOTRYPSIN
92
9.3.3 ADSORPTION OF CHYMOTRYPSIN ON
*
*
2
AND **
2 0 3
COLLOIDAL PARTICLES 93
9.3.4 ADSORPTION/ DESORPTION STUDIES OF CHYMOTRYPSIN 94
9.3.5
ENZYMATIC ACTIVITY OF ADSORBED CHYMOTRYPSIN
96
9.4 DISCUSSION 98
9.5 CONCLUSIONS 101
9.6 ACKNOWLEDGMENTS 102
10 PHYSJSORPTION OF *-CHYMOTRYPS*N ON SI02 AND **
*
2
: A COMPARATIVE STUDY
VIA EXPERIMENTS AND MOLECULAR DYNAMICS SIMULATIONS 103
10.1 INTRODUCTION 103
10.2 MATERIALS AND METHODS 106
10.2.1 MATERIALS 106
10.2.2 CHARACTERIZATION OF SI02 AND **
*
2
106
10.2.3 MALDI-TOF-MS 107
10.2.4 TIME- AND PH-DEPENDENT ADSORPTION OF CHYMOTRYPSIN TO
*
*
2
ANDTI
*
2
108
10.2.5 CONCENTRATION-DEPENDENT ADSORPTJON OF CHYMOTRYPSJN TO SI
*
2
ANDTI
*
2
109
10.2.6 DESORPTION STUDIES 110
10.2.7 MOLECULAR DYNAMICS SIMULATIONS 110
10.3 RESULTS 113
10.3.1 CHARACTERIZATION OF SI02 AND
* *
2
COLLOIDAL PARTICLES 113
10.3.2 DEPENDENCE OF THE PROTEIN ADSORPTION ON INCUBATJON TIME AND
PH 117
10.3.3 MALDI-TOF-MS ANALYSIS OF POSSIBLE AUTOLYSIS 118
10.3.4 ANALYSIS OF THE ADSORPTION LAYERS 120
10.3.5 DEPENDENCE OF THE ADSORBED AMOUNT ON THE PROTEIN
CONCENTRATION 122
10.3.6 LONG-RANGE INTERACTION FORCE OF CHYMOTRYPSIN OVER S*02 AND *.102
123
10.3.7 INTERACTION FORCES FOR CHYMOTRYPSIN MULTI-LAYERS 126
10.3.8 EXPL*CIT-SOLVENT MD SIMULATIONS OF CHYMOTRYPSIN ADSORPTION ON
TI02AND SI
*
2
128
10.4 DISCUSSION AND CONCLUSIONS 132
10.5 ACKNOWLEDGMENTS 135
10.6 APPENDIX: CIRCULAR DICHRO^^ 135
10.7 SOURCE 138
11 ASSESSMENT OF THE PROTEOLYTIC ACTIVITY OF A-CHYMOTRYPS*N IMMOBILIZED
ON
COLLOIDAL PARTICLES BY MATR*X*ASS*STED LASER DESORPTION IONIZATION
T*ME*O
*
*
FLIGHT MASS SPECTROSCOPY 140
11.1 INTRODUCTION 140
11.2 MATERIALS AND METHODS 142
11.2.1 MATERIALS 142
11.2.2 CHARACTERIZATION OF ALUMINA AND SILICA COLLOIDAL PARTICLES 143
11.2.3 CHYMOTRYPSIN IMMOBILIZATION ON ALUMINA AND SILICA COLLOIDAL
PARTICLES 144
11.2.4 PROTEOLYSIS TEST BY MALDI-TOF*MS MEASUREMENTS 146
11.2.5 DATA ANALYSIS 147
11.3 RESULTS 148
11.4 DISCUSSION 156
11.5 CONCLUSIONS 160
11.6 ACKNOWLEDGMENTS 161
11.7 APPENDIX: CHARACTERIZATION OF COLOIDAL PARTJCLES AFTER SURFACE
FUNCTIONALIZATION AND ENZYME MMOBILJZATJON USJNG FOURIER TRANSFORM
INFRARED (FIR ) SPECTROSCOPY 161
11.8 SOURCE 163
12 CONCLUSIONS 164
1 3 0 U TLO O K 168
LIST OF STUDENTS PROJECTS 170
LI*T OF PUBLICATIONS 171
SELECTED ORAL AND POSTER PRESENTATIONS 172
14 REFERENCES 173
|
| any_adam_object | 1 |
| author | Derr, Ludmilla 1984- |
| author_GND | (DE-588)1107057949 |
| author_facet | Derr, Ludmilla 1984- |
| author_role | aut |
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| author_variant | l d ld |
| building | Verbundindex |
| bvnumber | BV043720420 |
| classification_rvk | VK 8700 |
| classification_tum | CHE 827d |
| ctrlnum | (OCoLC)957467910 (DE-599)BVBBV043720420 |
| discipline | Chemie / Pharmazie Biologie |
| edition | Als Manuskript gedruckt |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV043720420 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T05:13:36Z |
| institution | BVB |
| isbn | 9783183760053 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029132493 |
| oclc_num | 957467910 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-83 DE-210 |
| owner_facet | DE-91G DE-BY-TUM DE-83 DE-210 |
| physical | XIX, 189 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| publisher | VDI Verlag GmbH |
| record_format | marc |
| series | Fortschritt-Berichte VDI. Reihe 5, Grund- und Werkstoffe, Kunststoffe |
| series2 | Fortschritt-Berichte VDI. Reihe 5, Grund- und Werkstoffe, Kunststoffe |
| spellingShingle | Derr, Ludmilla 1984- Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity Fortschritt-Berichte VDI. Reihe 5, Grund- und Werkstoffe, Kunststoffe Immobilisiertes Enzym (DE-588)4161361-2 gnd Physisorption (DE-588)4123950-7 gnd Oxidkeramik (DE-588)4173025-2 gnd Chymotrypsin (DE-588)4148026-0 gnd Enzymaktivität (DE-588)4152470-6 gnd |
| subject_GND | (DE-588)4161361-2 (DE-588)4123950-7 (DE-588)4173025-2 (DE-588)4148026-0 (DE-588)4152470-6 (DE-588)4113937-9 |
| title | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity |
| title_alt | Interactions: enzymes and colloidal particles |
| title_auth | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity |
| title_exact_search | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity |
| title_full | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity Dipl.-Chem. Ludmilla Derr, Bremen |
| title_fullStr | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity Dipl.-Chem. Ludmilla Derr, Bremen |
| title_full_unstemmed | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity Dipl.-Chem. Ludmilla Derr, Bremen |
| title_short | Interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity |
| title_sort | interactions between enzymes and oxide colloidal particles and their influence on enzymatic activity |
| topic | Immobilisiertes Enzym (DE-588)4161361-2 gnd Physisorption (DE-588)4123950-7 gnd Oxidkeramik (DE-588)4173025-2 gnd Chymotrypsin (DE-588)4148026-0 gnd Enzymaktivität (DE-588)4152470-6 gnd |
| topic_facet | Immobilisiertes Enzym Physisorption Oxidkeramik Chymotrypsin Enzymaktivität Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029132493&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000016442 |
| work_keys_str_mv | AT derrludmilla interactionsbetweenenzymesandoxidecolloidalparticlesandtheirinfluenceonenzymaticactivity AT derrludmilla interactionsenzymesandcolloidalparticles |