GFP whole cell microbial biosensors scale-up and scale-down effects on biopharmaceutical processes
Gespeichert in:
| Format: | Elektronisch E-Book |
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| Sprache: | English |
| Veröffentlicht: |
New York, N.Y.
ASME
2013
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| Schriftenreihe: | Biomedical & nanomedical technologies
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| Schlagworte: | |
| Online-Zugang: | DE-1046 DE-1047 Volltext |
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| Beschreibung: | Title from PDF t.p. (viewed on January 26, 2013) Includes bibliographical references (p. 36-43) 1. Interaction between fluid flow and microbial cells: importance of the operating scale -- 1.1 Different scales involved in the development of microbial bioprocesses -- 1.2 Hydrodynamic modeling of the substrate heterogeneites perceived by cells -- 2. Stochastic simulation of the displacement of microbial cells along concentration field -- 2.1 Microbial stress response: different timescales involved -- 3. Experimental results gained from the physiological response of GFP biosensors in scale-down conditions -- 3.1 General stress response in scale-down bioreactors: rpoS::GFP -- 3.2 Use of destabilized GFP variants -- 4. Another source of information: protein leakage and the study of the secretome -- References Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters |
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| Beschreibung: | 1 electronic text (43 p.) |
| ISBN: | 1606504177 1606504274 9781606504178 9781606504277 |