Glyco-Engineering Methods and Protocols

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Veröffentlicht: New York, NY Springer New York 2015
Schriftenreihe:Methods in Molecular Biology 1321
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Datensatz im Suchindex

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adam_text GLYCO-ENGINEERING / : 2015 TABLE OF CONTENTS / INHALTSVERZEICHNIS CURRENT APPROACHES TO ENGINEERING N-LINKED PROTEIN GLYCOSYLATION IN BACTERIA INVERSE METABOLIC ENGINEERING FOR ENHANCED GLYCOPROTEIN PRODUCTION IN ESCHERICHIA COLI GLYCOSNAP: A HIGH-THROUGHPUT SCREENING METHODOLOGY FOR ENGINEERING DESIGNER GLYCOSYLATION ENZYMES PRODUCTION OF GLYCOPROTEINS WITH ASPARAGINE-LINKED N-ACETYLGLUCOSAMINE IN ESCHERICHIA COLI GLYCO-ENGINEERING O-ANTIGENE-BASED VACCINES AND DIAGNOSTICS IN E. COLI PROGRESS IN YEAST GLYCOSYLATION ENGINEERING PROTEIN PRODUCTION WITH A PICHIA PASTORIS OCH1 KNOCK-OUT STRAIN IN FED-BATCH MODE ENGINEERING THE PICHIA PASTORIS N-GLYCOSYLATION PATHWAY USING THE GLYCOSWITCH TECHNOLOGY DEVELOPMENT OF A VALUABLE YEAST STRAIN USING A NOVEL MUTAGENESIS TECHNIQUE FOR THE EFFECTIVE PRODUCTION OF THERAPEUTIC GLYCOPROTEINS AN OVERVIEW AND HISTORY OF GLYCO-ENGINEERING IN INSECT EXPRESSION SYSTEMS SWEETBAC: APPLYING MULTIBAC TECHNOLOGY TOWARDS FLEXIBLE MODIFICATION OF INSECT CELL GLYCOSYLATION ENGINEERING SIALIC ACID SYNTHESIS ABILITY IN INSECT CELLS ENGINEERING N-GLYCOSYLATION PATHWAY IN INSECT CELLS: SUPPRESSION OF Β-N-ACETYLGLUCOSAMINIDASE AND EXPRESSION OF Β-1,4-GALACTOSYLTRANSFERASE N-GLYCO-ENGINEERING IN PLANTS: UPDATE ON STRATEGIES AND MAJOR ACHIEVEMENTS GENE TARGETING FOR PRECISION GLYCO-ENGINEERING: PRODUCTION OF BIOPHARMACEUTICALS DEVOID OF PLANT-TYPICAL GLYCOSYLATION IN MOSS BIOREACTORS IM“PLANT”ING OF MAMMALIAN GLYCOSYLTRANSFERASE GENE INTO PLANT SUSPENSION-CULTURED CELLS USING AGROBACTERIUM-MEDIATED TRANSFORMATION TRANSIENT GLYCO-ENGINEERING OF N. BENTHAMIANA AIMING AT THE SYNTHESIS OF MULTI-ANTENNARY SIALYLATED PROTEINS SUBCELLULAR TARGETING OF PROTEINS INVOLVED IN MODIFICATION OF PLANT N- AND O-GLYCOSYLATION ASSEMBLY OF MULTIGENE CONSTRUCTS USING GOLDEN GATE CLONING STRATEGIES FOR ENGINEERING PROTEIN N-GLYCOSYLATION PATHWAY IN MAMMALIAN CELLS GLYCAN REMODELLING WITH PROCESSING INHIBITORS AND LECTIN-RESISTANT EUKARYOTIC CELLS PRODUCTION OF HIGHLY SIALYLATED RECOMBINANT GLYCOPROTEINS USING RICINUS COMMUNIS AGGLUTININ-I-RESISTANT CHO GLYCOSYLATION MUTANTS METABOLIC GLYCO-ENGINEERING IN EUKARYOTIC CELLS AND SELECTED APPLICATIONS EVALUATION OF QUENCHING AND EXTRACTION METHODS FOR NUCLEOTIDE/NUCLEOTIDE SUGAR ANALYSIS CHEMOENZYMATIC GLYCO-ENGINEERING OF MONOCLONAL ANTIBODIES CHEMICAL POLYSIALYLATION OF RECOMBINANT HUMAN PROTEINS SITE SPECIFIC GLYCOSYLATION PROFILING USING LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS) MASS SPECTROMETRIC ANALYSIS OF OLIGO- AND POLYSIALIC ACIDS ISOMER-SPECIFIC ANALYSIS OF RELEASED N-GLYCANS BY LC-ESI MS/MS WITH POROUS GRAPHITIZED CARBON DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT. GLYCO-ENGINEERING / : 2015 ABSTRACT / INHALTSTEXT CONCEIVED WITH THE INTENTION OF PROVIDING AN ARRAY OF STRATEGIES AND TECHNOLOGIES CURRENTLY IN USE FOR GLYCO-ENGINEERING DISTINCT LIVING ORGANISMS, THIS BOOK CONTAINS A WIDE RANGE OF METHODS BEING DEVELOPED TO CONTROL THE COMPOSITION OF CARBOHYDRATES AND THE PROPERTIES OF PROTEINS THROUGH MANIPULATIONS ON THE PRODUCTION HOST RATHER THAN IN THE PROTEIN ITSELF. THE FIRST FIVE SECTIONS DEAL WITH HOST-SPECIFIC GLYCO-ENGINEERING AND CONTAIN CHAPTERS THAT PROVIDE PROTOCOLS FOR MODIFICATIONS OF THE GLYCOSYLATION PATHWAY IN BACTERIA, YEAST, INSECT, PLANTS, AND MAMMALIAN CELLS, WHILE THE LAST TWO SECTIONS EXPLORE ALTERNATIVE APPROACHES TO HOST GLYCO-ENGINEERING AND SELECTED PROTOCOLS FOR THE ANALYSIS OF THE N-GLYCANS AND GLYCO-PROFILING BY MASS SPECTROMETRY. WRITTEN FOR THE HIGHLY SUCCESSFUL METHODS IN MOLECULAR BIOLOGY SERIES, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS. AUTHORITATIVE AND EXTENSIVE, GLYCO-ENGINEERING: METHODS AND PROTOCOLS OFFERS VAST OPTIONS TO HELP RESEARCHERS TO CHOOSE THE EXPRESSION SYSTEM AND APPROACH THAT BEST SUITS THEIR INTENDED PROTEIN RESEARCH OR APPLICATIONS DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
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spellingShingle Glyco-Engineering Methods and Protocols
Life sciences
Biochemistry
Life Sciences
Protein Science
Biowissenschaften
title Glyco-Engineering Methods and Protocols
title_auth Glyco-Engineering Methods and Protocols
title_exact_search Glyco-Engineering Methods and Protocols
title_full Glyco-Engineering Methods and Protocols edited by Alexandra Castilho
title_fullStr Glyco-Engineering Methods and Protocols edited by Alexandra Castilho
title_full_unstemmed Glyco-Engineering Methods and Protocols edited by Alexandra Castilho
title_short Glyco-Engineering
title_sort glyco engineering methods and protocols
title_sub Methods and Protocols
topic Life sciences
Biochemistry
Life Sciences
Protein Science
Biowissenschaften
topic_facet Life sciences
Biochemistry
Life Sciences
Protein Science
Biowissenschaften
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