Methods in cilia & flagella

Gespeichert in:
Bibliographische Detailangaben
Weitere Verfasser: Basto, Renata (HerausgeberIn)
Format: Buch
Sprache:English
Veröffentlicht: Amsterdam [u.a.] Elsevier, Academic Press 2015
Ausgabe:1. ed.
Schriftenreihe:Methods in cell biology 127
Schlagworte:
Online-Zugang:Inhaltsverzeichnis
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

MARC

LEADER 00000nam a2200000 cb4500
001 BV042549621
003 DE-604
005 20150518
007 t|
008 150508s2015 xx ad|| |||| 00||| eng d
020 |a 9780128024515  |9 978-0-12-802451-5 
035 |a (OCoLC)907572157 
035 |a (DE-599)BVBBV042549621 
040 |a DE-604  |b ger 
041 0 |a eng 
049 |a DE-355  |a DE-29T  |a DE-11  |a DE-703 
084 |a WC 5100  |0 (DE-625)148108:13423  |2 rvk 
245 1 0 |a Methods in cilia & flagella  |c ed. by Renata Basto ... 
250 |a 1. ed. 
264 1 |a Amsterdam [u.a.]  |b Elsevier, Academic Press  |c 2015 
300 |a XXII, 567 S., [12] Bl.  |b Ill., graph. Darst. 
336 |b txt  |2 rdacontent 
337 |b n  |2 rdamedia 
338 |b nc  |2 rdacarrier 
490 1 |a Methods in cell biology  |v 127 
650 0 7 |a Cytologie  |0 (DE-588)4070177-3  |2 gnd  |9 rswk-swf 
650 0 7 |a Zilie  |0 (DE-588)4190826-0  |2 gnd  |9 rswk-swf 
650 0 7 |a Methode  |0 (DE-588)4038971-6  |2 gnd  |9 rswk-swf 
650 0 7 |a Geißel  |g Biologie  |0 (DE-588)4156348-7  |2 gnd  |9 rswk-swf 
689 0 0 |a Zilie  |0 (DE-588)4190826-0  |D s 
689 0 1 |a Cytologie  |0 (DE-588)4070177-3  |D s 
689 0 2 |a Methode  |0 (DE-588)4038971-6  |D s 
689 0 |5 DE-604 
689 1 0 |a Geißel  |g Biologie  |0 (DE-588)4156348-7  |D s 
689 1 1 |a Cytologie  |0 (DE-588)4070177-3  |D s 
689 1 2 |a Methode  |0 (DE-588)4038971-6  |D s 
689 1 |C b  |5 DE-604 
700 1 |a Basto, Renata  |4 edt 
830 0 |a Methods in cell biology  |v 127  |w (DE-604)BV002534878  |9 127 
856 4 2 |m Digitalisierung UB Regensburg - ADAM Catalogue Enrichment  |q application/pdf  |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027983531&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA  |3 Inhaltsverzeichnis 
943 1 |a oai:aleph.bib-bvb.de:BVB01-027983531 

Datensatz im Suchindex

_version_ 1819705765187813376
adam_text Contents Contributors ..........................................................................................xiii Preface ................................................................................................xxi CHAPTER 1 Imaging Centrosomes and Cilia in the Mouse Kidney ....................................................................1 Masato Hoshi, Jinzhi Wang, Sanjay Jain, Мое R. Mahjoub Introduction .......................................................................2 1. Methods ...........................................................................4 Conclusion ......................................................................13 References ......................................................................14 CHAPTER 2 Ependymal Cell Differentiation, from Monociliated to Multiciliated Cells ..............................................19 Nathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehi, Carsten Janke, Nathalie Spassky Introduction..................................................................... 20 1. Methods .........................................................................20 Conclusion ......................................................................32 Acknowledgments ............................................................32 References ......................................................................32 CHAPTER 3 Observing Planar Cell Polarity in Multiciliated Mouse Airway Epithelial Cells ................................37 Eszter К. Vladar, Yin Loon Lee, Tim Stearns, Jeffrey Đ. Axelrod Introduction..................................................................... 38 1. Acquisition or Generation of Multiciliated Airway Epithelial Cells ..............................................................................41 2. Assessment of Ultrastructural Planar Polarity of Motile Cilia... 42 3. Localization of PCP Signaling Pathway Proteins in Multiciliated Airway Epithelial Cells ...................................48 Conclusions .....................................................................52 Acknowledgments ............................................................52 References ......................................................................52 vi Contents CHAPTER 4 Imaging and Analyzing Primary Cilia in Cardiac Cells ......................................................................55 Nicolas Diguet, Jean-François Le Garree, Tommaso Lucchesi, Sigolène M. Meilhac Introduction .....................................................................56 1. Imaging Cilia In situ, in Fixed Hearts ..................................57 2. Imaging and Manipulating Cilia in Primary Cultures of Cardiac Cells ...............................................................61 3. Quantitative Analysis of Cilium Orientation ..........................67 Conclusion ......................................................................72 Acknowledgments ............................................................72 Supplementary Data ..........................................................72 References ......................................................................72 CHAPTER 5 Cilia in Photoreceptors ...........................................75 Linjing Li, Manistia Anand, Kollu Nageswara Rao, Hemant Khanna Introduction .....................................................................76 1. Methods .........................................................................77 Conclusions .....................................................................91 Acknowledgments ............................................................91 References ......................................................................92 CHAPTER 6 Analysis of Primary Cilia in the Developing Mouse Brain ..........................................................93 Judith T.M.L Paridaen, Wieland B. Huttner, Michaela Wilsch-Bräuninger Introduction .....................................................................94 1. Neurogenesis in the Developing Neocortex ...........................94 2. Primary Cilia in the Developing Neocortex ...........................96 3. Material ..........................................................................97 4. Methods .......................................................................103 Conclusion ....................................................................126 Acknowledgments ..........................................................126 References ....................................................................126 CHAPTER 7 In Vivo Investigation of Cilia Structure and Function Using Xenopus .......................................131 Eric R. Brooks, John B. Wallingford Introduction ...................................................................132 1. A Brief Introduction to Xenopus Husbandry and Manipulation .................................................................133 Contents vii 2. Imaging of Cilia in Fixed Xenopus Embryos and Tissues ...... 134 3. Methods for Live Cell Analysis of Cilia in Xenopus ............. 139 4. Mounting Xenopus Embryos ............................................ 142 5. Image Acquisition .......................................................... 145 6. Quantitative Analysis of Ciliary Properties .........................151 Conclusion ....................................................................154 Acknowledgments .......................................................... 155 References .................................................................... 155 CHAPTER 8 A Quantitative Approach to Study Endothelial Cilia Bending Stiffness During Blood Flow Mechanodetection In Vivo .................................... 161 Francesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot Introduction ................................................................... 162 1. The Challenge of Measuring Ciliary Mechanics .................. 162 2. Live Imaging ................................................................. 164 3. Image Processing ........................................................... 168 4. Bending Rigidity Estimation through Modeling ................... 168 5. Material ........................................................................ 172 Conclusion .................................................................... 172 Acknowledgments .......................................................... 173 References .................................................................... 173 CHAPTER 9 Quantitative Description of Fluid Flows Produced by Left-Right Cilia in Zebrafish ............................175 Craig Fox, M. Lisa Manning, Jeffrey D. Amack Introduction ................................................................... 176 1. Methods .......................................................................177 2. Application ...................................................................183 Conclusion .................................................................... 185 Acknowledgments .......................................................... 185 References .................................................................... 185 CHAPTER 10 Efficient Live Fluorescence Imaging of Intraflagellar Transport in Mammalian Primary Cilia ........................................................189 Hiroaki Ishikawa, Wallace F. Marshall Introduction ................................................................... 190 1. Preparation of Cells for IFT Imaging ................................. 191 2. Live Imaging of IFT ....................................................... 193 viii Contents 3. Image Processing and Data Analysis .................................195 4. Discussion.................................................................... 198 Acknowledgments ..........................................................199 Supplementary Data ........................................................199 References ....................................................................199 CHAPTER 11 Analysis of Soluble Protein Entry Into Primary Cilia Using Semipermeabilized Cells ....................203 David K. Breslow, Maxence V. Nachury Introduction ...................................................................204 1. Methods .......................................................................205 2. Conclusions and Future Outlook .......................................219 Acknowledgments ..........................................................219 References ....................................................................220 CHAPTER 12 Methods for Imaging Individual Cilia in Living Echinoid Embryos .................................................223 Robert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty Introduction ...................................................................224 1, Methods .......................................................................225 Conclusion ....................................................................240 Acknowledgments ..........................................................240 Supplementary Data ........................................................240 References ....................................................................240 CHAPTER 13 The planarian Schmidtea Mediterranea as a Model for Studying Motile Cilia and Multiciliated Cells ...............................................243 Cyril Basquin, Anne-Marie Orfila, Juliette Azimzadeh Introduction ...................................................................244 1. Maintaining Planarians in the Lab .....................................247 2. RNA Interference ...........................................................251 3. Whole-Mount Immunofluorescence Staining .......................255 4. Electron Microscopy .......................................................258 5. Live Imaging .................................................................260 Acknowledgments ..........................................................261 References ....................................................................261 Contents ix CHAPTER 14 Quantitative Analysis of Flagellar Proteins in Drosophila Sperm Tails ........................................263 Teresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto Introduction ...................................................................264 1. Methods .......................................................................266 Concluding Remarks .......................................................276 Acknowledgments ..........................................................276 References ....................................................................276 CHAPTER 15 Imaging Cilia in Drosophila melanogaster .............279 Jennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Bénédicte Durand Introduction ...................................................................280 1. Immunodetection of Cilia ................................................283 2. Observation of Cilia Ultrastructure by Transmission Electron Microscopy ...................................................................293 Acknowledgments ..........................................................298 References ....................................................................298 CHAPTER 16 Studying G Protein-coupled Receptors: Immunoblotting, Immunoprecipitation, Phosphorylation, Surface Labeling, and Cross-linking Protocols ........................................ зоз Kasturi Pal, Hemant Badgandi, Saikat Mukhopadhyay Introduction ...................................................................305 1. Reagents .......................................................................307 2. Protocol for Immunoblotting GPCRs .................................311 3. Protocol for Immunoprecipitating GPCRs ...........................313 4. GPCR Labeling and Cross-Linking Studies .........................317 5. Comments on Biochemical Assays Involving GPCRs and Multipass Transmembrane Proteins ...................................319 Conclusions ...................................................................320 Acknowledgments ..........................................................320 References ....................................................................320 CHAPTER 17 image Analysis of Caenorhabditis elegans Ciliary Transition Zone Structure, Ultrastructure, Molecular Composition, and Function ................... 323 Anna A.W.M. Sanders, Julie Kennedy, Oliver E. Blacque Introduction ...................................................................324 Contents 1. Cilium Compartmentalization and the Transition Zone.........324 2. Caenorhabditis elegans Sensory Neuronal Cilia Model.........326 3. Methods....................................................................... 328 Concluding Remarks .......................................................344 References ....................................................................344 CHAPTER 18 Genetic and Genomic Approaches to Identify Genes Involved in Flagellar Assembly in Chlamydomonas Reinhardt!!.................................349 Huawen Lin, Susan K. Dutcher Introduction ...................................................................350 1. Identifying the Causative Genes Using Forward Genetics ......351 2. Identifying Gene Function Using Reverse Genetics ..............370 3. Identifying Genes Using the RNA Transcriptome................. 373 4. Identifying Genes Using Genetic Interactions ......................375 5. Summary ......................................................................378 Acknowledgments ..........................................................378 References ....................................................................378 CHAPTER 19 Axonemal Motility in Chlamydomonas ...................387 Ken-ichi Wakabayashi, Ritsu Kamiya Introduction ...................................................................388 1. Methods .......................................................................389 Acknowledgments ..........................................................400 References ....................................................................400 CHAPTER 20 Posttranscriptional Control Over Rapid Development and Ciliogenesis in Marsilea ............403 Stephen M. Wolniak, Thomas С Boothby, Corine M. van der Weele Introduction ...................................................................404 1. The Development of Experimental Protocols in a Nonmodel System ..........................................................409 2. Centrin Translation is Necessary for Formation of the Blepharoplast ................................................................412 3. Cell Fate in the Male Gametophyte of Marsilea: Only Two Types of Cells are Produced ......................................415 4. Spermidine Provides a Driving Force behind Rapid Development in the Male Gametophyte .............................417 5. Spermidine Levels Change in the Gametophyte ...................418 Contents xi 6. Stored mRNAs are Transcribed during the Natural Process of Spore Desiccation .......................................................420 7. Mago Nashi and Speckle mRNA Dynamics ........................423 8. Emerging Themes from the Transcriptome of the Rapidly Developing Male Gametophyte of Mar silea ........................423 9. Many of the Stored Transcripts in the Spore Nucleus are Partially Processed Pre-mRNAs ........................................425 10. NudC, an Archetype IRT in the Gametophyte .....................426 11. IRT Splicing as a Means to Regulate the Timing and Location of Translation ...................................................427 12. Polyadeny lation as a Late Regulator of Translation ..............430 Conclusions, Predictions, and a Working Model for the Control of Rapid Development .........................................430 Acknowledgments ..........................................................438 References ....................................................................438 CHAPTER 21 Total Internal Reflection Fluorescence Microscopy of Intraflagellar Transport in Tetrahymena thermophila .........................................................445 Yu-Yang Jiang, Karl Lechtreck, Jacek Gaertig Introduction ...................................................................446 1. TIRF Microscope Configuration and Data Analysis in Application to IFT in Tetrahymena ....................................447 2. Using a TIRF microscope to Image Tetrahymena cilia ..........447 3. Limitations ....................................................................453 Acknowledgments ..........................................................454 Supplementary Data ........................................................454 References ....................................................................454 CHAPTER 22 Ciliary Heterogeneity within a Single Cell: The Рагатесіит Model ..............................................457 Anne Aubusson-Fleury, Jean Cohen, Michel Lemullois 1. The Paramecium Model ..................................................458 2. Cilia in Paramecium Organization .....................................460 3. Staining the Cilia with Antitubulin Antibodies ....................465 4. Protocols ......................................................................470 5. Conclusion ....................................................................478 6. Chemical Reagents and Buffers ........................................479 Acknowledgments ..........................................................479 References ....................................................................479 xii Contents CHAPTER 23 Imaging Intraflagellar Transport in Trypanosomes ......................................................487 Julien Santi-Rocca, Nicolas Chenouard, Cécile Fort, Thibault Lagache, Jean-Christophe Olivo-Marin, Philippe Bastin Introduction ...................................................................488 1. Producing Trypanosomes Expressing Fluorescent IFT Fusion Proteins ........................................................490 2. Visualization of IFT and Acquisition of Time-Lapse Videos .. 498 3. Quantification of IFT ......................................................500 Conclusion ....................................................................504 Acknowledgments ..........................................................504 Supplementary Data ........................................................504 References ....................................................................504 CHAPTER 24 Scanning and Three-Dimensional Electron Microscopy Methods for the Study of Trypanosoma brucei and Leishmania mexicana Flagella............................................... .509 Eva Gluenz, Richard John Wheeler, Louise Hughes, Sue Vaughan Introduction ...................................................................510 1. Rationale ......................................................................515 2. Methods .......................................................................516 3. Materials ......................................................................535 4. Discussion ....................................................................537 Acknowledgments ..........................................................538 References ....................................................................538 Volumes in Series .................................................................................543 Index ..................................................................................................555
any_adam_object 1
author2 Basto, Renata
author2_role edt
author2_variant r b rb
author_facet Basto, Renata
building Verbundindex
bvnumber BV042549621
classification_rvk WC 5100
ctrlnum (OCoLC)907572157
(DE-599)BVBBV042549621
discipline Biologie
edition 1. ed.
format Book
fullrecord <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01807nam a2200457 cb4500</leader><controlfield tag="001">BV042549621</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20150518 </controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">150508s2015 xx ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780128024515</subfield><subfield code="9">978-0-12-802451-5</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)907572157</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV042549621</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-703</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5100</subfield><subfield code="0">(DE-625)148108:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Methods in cilia &amp; flagella</subfield><subfield code="c">ed. by Renata Basto ...</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Academic Press</subfield><subfield code="c">2015</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XXII, 567 S., [12] Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in cell biology</subfield><subfield code="v">127</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zilie</subfield><subfield code="0">(DE-588)4190826-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Geißel</subfield><subfield code="g">Biologie</subfield><subfield code="0">(DE-588)4156348-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Zilie</subfield><subfield code="0">(DE-588)4190826-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Geißel</subfield><subfield code="g">Biologie</subfield><subfield code="0">(DE-588)4156348-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="2"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Basto, Renata</subfield><subfield code="4">edt</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in cell biology</subfield><subfield code="v">127</subfield><subfield code="w">(DE-604)BV002534878</subfield><subfield code="9">127</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg - ADAM Catalogue Enrichment</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&amp;doc_library=BVB01&amp;local_base=BVB01&amp;doc_number=027983531&amp;sequence=000002&amp;line_number=0001&amp;func_code=DB_RECORDS&amp;service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-027983531</subfield></datafield></record></collection>
id DE-604.BV042549621
illustrated Illustrated
indexdate 2024-12-24T04:27:16Z
institution BVB
isbn 9780128024515
language English
oai_aleph_id oai:aleph.bib-bvb.de:BVB01-027983531
oclc_num 907572157
open_access_boolean
owner DE-355
DE-BY-UBR
DE-29T
DE-11
DE-703
owner_facet DE-355
DE-BY-UBR
DE-29T
DE-11
DE-703
physical XXII, 567 S., [12] Bl. Ill., graph. Darst.
publishDate 2015
publishDateSearch 2015
publishDateSort 2015
publisher Elsevier, Academic Press
record_format marc
series Methods in cell biology
series2 Methods in cell biology
spellingShingle Methods in cilia & flagella
Methods in cell biology
Cytologie (DE-588)4070177-3 gnd
Zilie (DE-588)4190826-0 gnd
Methode (DE-588)4038971-6 gnd
Geißel Biologie (DE-588)4156348-7 gnd
subject_GND (DE-588)4070177-3
(DE-588)4190826-0
(DE-588)4038971-6
(DE-588)4156348-7
title Methods in cilia & flagella
title_auth Methods in cilia & flagella
title_exact_search Methods in cilia & flagella
title_full Methods in cilia & flagella ed. by Renata Basto ...
title_fullStr Methods in cilia & flagella ed. by Renata Basto ...
title_full_unstemmed Methods in cilia & flagella ed. by Renata Basto ...
title_short Methods in cilia & flagella
title_sort methods in cilia flagella
topic Cytologie (DE-588)4070177-3 gnd
Zilie (DE-588)4190826-0 gnd
Methode (DE-588)4038971-6 gnd
Geißel Biologie (DE-588)4156348-7 gnd
topic_facet Cytologie
Zilie
Methode
Geißel Biologie
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027983531&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link (DE-604)BV002534878
work_keys_str_mv AT bastorenata methodsinciliaflagella