Mycobacteria protocols
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
New York [u.a.]
Humana Press
2015
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Ausgabe: | 3. ed. |
Schriftenreihe: | Methods in molecular biology
465 |
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Online-Zugang: | Inhaltsverzeichnis |
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264 | 1 | |a New York [u.a.] |b Humana Press |c 2015 | |
300 | |a XII, 416 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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adam_text | Titel: Mycobacteria protocols
Autor: Parish, Tanya
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... ix
1 Whole-Genome Sequencing for Comparative Genomics
and De Novo Genome Assembly................................... 1
Andrej Benjak, Claudia Sala, and Kuben C. Hartkoorn
2 Whole-Transcriptome Sequencing for High-Resolution Transcriptomic
Analysis in Mycobacterium tuberculosis............................... 17
Andrej Benjak, Claudia Sala, and Kuben C. Hartkoorn
3 RNA Sequencing for Transcript 5 -End Mapping in Mycobacteria.......... 31
ScarletS. Shell, Michael K Chase, Thomas K Ioerger,
and Sarah M. Fortune
4 Fractionation and Analysis of Mycobacterial Proteins.................... 47
Megan C. Lucas, Lisa M. Wolfe, Rachel M. Hazenfield, Jade Kurihara,
Nicole A. Kruh-Garcia, John Belisle, and Karen M. Dobos
5 Lipid and Lipoarabinomannan Isolation and Characterization............. 77
Marie-Antoinette Lanéelle, Jérôme Nigou, and Mamadou Daffê
6 Metabolomics of Mycobacterium tuberculosis.......................... 105
Madhumitha Nandakumar, Gareth A. Prosser,
Luiz Pedro S. de Carvalho, and Kyu Rhee
7 Electroporation of Mycobacteria................................... 117
Renan Goude, David M. Roberts, and Tanya Parish
8 Targeted Gene Knockout and Essentiality Testing
by Homologous Recombination................................... 131
Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi
9 Construction of Conditional Knockdown Mutants in Mycobacteria......... 151
Dirk Schnappinger, Kathryn M. O Brien, and Sabine Ehrt
10 Mycobacterial Recombineering.................................... 177
Kenan C. Murphy, Kadamba Papavinasasundaram,
and Christopher M. Sassetti
11 In Vitro Models That Utilize Hypoxia to Induce Non-replicating
Persistence in Mycobacteria....................................... 201
Charles D. Sohaskey and Martin I. Voskuil
12 Genetic Dissection of Mycobacterial Biofilms......................... 215
Anil K. Ojha, William R. Jacobs Jr., and Graham F. Hatfull
13 Measuring Efflux and Permeability in Mycobacteria.................... 227
Liliana Rodrigues, Miguel Viveiros, and José A. Ainsa
vii
viii
Contents
14 Single-Cell Analysis of Mycobacteria Using Microfluidics
and Time-Lapse Microscopy...................................... 241
Neeraj Dhar and Giulia Manina
15 Antimicrobial Susceptibility Testing for Mycobacterium sp................. 257
Delia Blanco-Ruano, David M. Roberts, Ruben Gonzalez-Del-Rio,
Daniel Alvarez, M* José Rebollo, Esther Pérez-Herrdn,
and Alfonso Mendoza
16 Determination of Compound Kill Kinetics Against
Mycobacterium tuberculosis....................................... 269
Julie Early and Torey Ailing
17 Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery
Assay (LORA) for Mycobacterium tuberculosis......................... 281
Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau
18 A Multi-stress Model for High Throughput Screening
Against Non-replicating Mycobacterium tuberculosis..................... 293
Ben Gold, Thulasi Warrier, and Carl Nathan
19 Isolation and Characterization of Compound-Resistant Isolates
of Mycobacterium tuberculosis..................................... 317
Theresa O Malley and Eduard Melief
20 Macrophage Infection Models for Mycobacterium tuberculosis............. 329
Benjamin K. Johnson and Robert B. Abramovitch
21 Infection of Human Neutrophils to Study Virulence Properties
of Mycobacterium tuberculosis..................................... 343
Tobias Dallenga, Björn Corleis, and Ulrich E. Schaible
22 Isolation of Bead Phagosomes to Study Virulence Function
of M. tuberculosis Cell Wall Lipids.................................. 357
Anna G Gejfken, Emmanuel C. Patin, and Ulrich E. Schaible
23 Live Imaging of Mycobacterium marinum Infection
in Dictyostelium discoideum....................................... 369
Caroline Barisch, Ana T. Lôpez-Jiménez, and Thierry Soldati
24 Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis
Infected Cells Using Phenotypic Assays.............................. 387
Vincent Delorme, Ok-Ryul Song, Alain Baulard, and Priscille Brodin
Index............................................................... 41S
|
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id | DE-604.BV042276611 |
illustrated | Illustrated |
indexdate | 2024-07-10T01:17:05Z |
institution | BVB |
isbn | 9781493924493 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027714070 |
oclc_num | 915554183 |
open_access_boolean | |
owner | DE-11 |
owner_facet | DE-11 |
physical | XII, 416 S. Ill., graph. Darst. |
publishDate | 2015 |
publishDateSearch | 2015 |
publishDateSort | 2015 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology |
spelling | Mycobacteria protocols ed. by Tanya Parish... 3. ed. New York [u.a.] Humana Press 2015 XII, 416 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 465 Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Mykobakterien (DE-588)4170927-5 gnd rswk-swf Mykobakterien (DE-588)4170927-5 s Molekulargenetik (DE-588)4039987-4 s Methode (DE-588)4038971-6 s DE-604 Parish, Tanya edt Methods in molecular biology 465 (DE-604)BV035362695 465 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027714070&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Mycobacteria protocols Methods in molecular biology Molekulargenetik (DE-588)4039987-4 gnd Methode (DE-588)4038971-6 gnd Mykobakterien (DE-588)4170927-5 gnd |
subject_GND | (DE-588)4039987-4 (DE-588)4038971-6 (DE-588)4170927-5 |
title | Mycobacteria protocols |
title_auth | Mycobacteria protocols |
title_exact_search | Mycobacteria protocols |
title_full | Mycobacteria protocols ed. by Tanya Parish... |
title_fullStr | Mycobacteria protocols ed. by Tanya Parish... |
title_full_unstemmed | Mycobacteria protocols ed. by Tanya Parish... |
title_short | Mycobacteria protocols |
title_sort | mycobacteria protocols |
topic | Molekulargenetik (DE-588)4039987-4 gnd Methode (DE-588)4038971-6 gnd Mykobakterien (DE-588)4170927-5 gnd |
topic_facet | Molekulargenetik Methode Mykobakterien |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027714070&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT parishtanya mycobacteriaprotocols |