Mycobacteria protocols

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Weitere Verfasser: Parish, Tanya (HerausgeberIn)
Format: Buch
Sprache:English
Veröffentlicht: New York [u.a.] Humana Press 2015
Ausgabe:3. ed.
Schriftenreihe:Methods in molecular biology 465
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adam_text Titel: Mycobacteria protocols Autor: Parish, Tanya Jahr: 2015 Contents Preface.......................................................... v Contributors.......................................................... ix 1 Whole-Genome Sequencing for Comparative Genomics and De Novo Genome Assembly................................... 1 Andrej Benjak, Claudia Sala, and Kuben C. Hartkoorn 2 Whole-Transcriptome Sequencing for High-Resolution Transcriptomic Analysis in Mycobacterium tuberculosis............................... 17 Andrej Benjak, Claudia Sala, and Kuben C. Hartkoorn 3 RNA Sequencing for Transcript 5 -End Mapping in Mycobacteria.......... 31 ScarletS. Shell, Michael K Chase, Thomas K Ioerger, and Sarah M. Fortune 4 Fractionation and Analysis of Mycobacterial Proteins.................... 47 Megan C. Lucas, Lisa M. Wolfe, Rachel M. Hazenfield, Jade Kurihara, Nicole A. Kruh-Garcia, John Belisle, and Karen M. Dobos 5 Lipid and Lipoarabinomannan Isolation and Characterization............. 77 Marie-Antoinette Lanéelle, Jérôme Nigou, and Mamadou Daffê 6 Metabolomics of Mycobacterium tuberculosis.......................... 105 Madhumitha Nandakumar, Gareth A. Prosser, Luiz Pedro S. de Carvalho, and Kyu Rhee 7 Electroporation of Mycobacteria................................... 117 Renan Goude, David M. Roberts, and Tanya Parish 8 Targeted Gene Knockout and Essentiality Testing by Homologous Recombination................................... 131 Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi 9 Construction of Conditional Knockdown Mutants in Mycobacteria......... 151 Dirk Schnappinger, Kathryn M. O Brien, and Sabine Ehrt 10 Mycobacterial Recombineering.................................... 177 Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti 11 In Vitro Models That Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria....................................... 201 Charles D. Sohaskey and Martin I. Voskuil 12 Genetic Dissection of Mycobacterial Biofilms......................... 215 Anil K. Ojha, William R. Jacobs Jr., and Graham F. Hatfull 13 Measuring Efflux and Permeability in Mycobacteria.................... 227 Liliana Rodrigues, Miguel Viveiros, and José A. Ainsa vii viii Contents 14 Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy...................................... 241 Neeraj Dhar and Giulia Manina 15 Antimicrobial Susceptibility Testing for Mycobacterium sp................. 257 Delia Blanco-Ruano, David M. Roberts, Ruben Gonzalez-Del-Rio, Daniel Alvarez, M* José Rebollo, Esther Pérez-Herrdn, and Alfonso Mendoza 16 Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis....................................... 269 Julie Early and Torey Ailing 17 Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis......................... 281 Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau 18 A Multi-stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis..................... 293 Ben Gold, Thulasi Warrier, and Carl Nathan 19 Isolation and Characterization of Compound-Resistant Isolates of Mycobacterium tuberculosis..................................... 317 Theresa O Malley and Eduard Melief 20 Macrophage Infection Models for Mycobacterium tuberculosis............. 329 Benjamin K. Johnson and Robert B. Abramovitch 21 Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis..................................... 343 Tobias Dallenga, Björn Corleis, and Ulrich E. Schaible 22 Isolation of Bead Phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids.................................. 357 Anna G Gejfken, Emmanuel C. Patin, and Ulrich E. Schaible 23 Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum....................................... 369 Caroline Barisch, Ana T. Lôpez-Jiménez, and Thierry Soldati 24 Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells Using Phenotypic Assays.............................. 387 Vincent Delorme, Ok-Ryul Song, Alain Baulard, and Priscille Brodin Index............................................................... 41S
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spelling Mycobacteria protocols ed. by Tanya Parish...
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XII, 416 S. Ill., graph. Darst.
txt rdacontent
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Parish, Tanya edt
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HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027714070&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle Mycobacteria protocols
Methods in molecular biology
Molekulargenetik (DE-588)4039987-4 gnd
Methode (DE-588)4038971-6 gnd
Mykobakterien (DE-588)4170927-5 gnd
subject_GND (DE-588)4039987-4
(DE-588)4038971-6
(DE-588)4170927-5
title Mycobacteria protocols
title_auth Mycobacteria protocols
title_exact_search Mycobacteria protocols
title_full Mycobacteria protocols ed. by Tanya Parish...
title_fullStr Mycobacteria protocols ed. by Tanya Parish...
title_full_unstemmed Mycobacteria protocols ed. by Tanya Parish...
title_short Mycobacteria protocols
title_sort mycobacteria protocols
topic Molekulargenetik (DE-588)4039987-4 gnd
Methode (DE-588)4038971-6 gnd
Mykobakterien (DE-588)4170927-5 gnd
topic_facet Molekulargenetik
Methode
Mykobakterien
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027714070&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link (DE-604)BV035362695
work_keys_str_mv AT parishtanya mycobacteriaprotocols