The evolutionary history of the mammalian synaptonemal complex
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2014
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| 245 | 1 | 0 | |a The evolutionary history of the mammalian synaptonemal complex |c von Johanna Fraune |
| 246 | 1 | 3 | |a Die Evolutionsgeschichte des Synaptonemalkomplexes der Maus |
| 264 | 1 | |c 2014 | |
| 300 | |a 149 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. und engl. Sprache | ||
| 502 | |a Würzburg, Univ., Diss., 2014 | ||
| 650 | 0 | 7 | |a Hydra |g Polyp |0 (DE-588)4160852-5 |2 gnd |9 rswk-swf |
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| 912 | |a ebook | ||
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Datensatz im Suchindex
| DE-BY-TUM_katkey | 2016800 |
|---|---|
| DE-BY-UBM_katkey | 5020514 |
| DE-BY-UBR_katkey | 5470944 |
| _version_ | 1823055509250375680 |
| adam_text | THE EVOLUTIONARY HISTORY OF THE
MAMMALIAN SYNAPTONEMAL
COMPLEX
DISSERTATION
ZUR ERLANGUNG DES NATURWISSENSCHAFTLICHEN DOKTORGRADES DER JULIUS-
MAXIMILIANS-UNIVERSITAT WURZBURG
VORGELEGT VON
JOHANNA FRAUNE
AUS HOXTER
WURZBURG, 2014
HTTP://D-NB.INFO/1058765744
INDEX
3
INDEX
INDEX 3
1 LIST OF FIGURES 6
2 LIST OF TABLES 7
3 SUMMARY 8
4 ZUSAMMENFASSUNG 9
5 GENERAL INTRODUCTION 11
5.1 MEIOSIS 11
5.1.1 MEIOSIS
I 11
5.1.2 MEIOSIS II 14
5.2 THE SYNAPTONEMAL
COMPLEX (SC) 15
5.2.1 THE SC COMPONENTS
OF METAZOAN MODEL ORGANISMS 16
5.2.2 THE FUNCTION OF SC COMPONENTS
AND THEIR ASSEMBLY TO A MATURE SC 20
6 AIMS OF THIS STUDY : 25
7 CHAPTER I: THE EVOLUTION OF THE TWO BONA
FIDE STRUCTURAL SC COMPONENTS OF THE MOUSE 27
INTRODUCTION 27
RESULTS 28
SEQUENCE AND STRUCTURE OF HYSYCPL
AND HYSYCP3 29
EXPRESSION OF HYSYCPL
AND HYSYCP3 IN
HYDRA VULGRISAEP
32
DISCUSSION 37
8 CHAPTER II:
THE EVOLUTION
OF THE
CENTRAL ELEMENT
COMPONENTS OF THE MURINE
SC 40
INTRODUCTION 40
RESULTS 40
PHYLOGENY OF SYCE1, SYCE2, SYCE3 AND TEXL2 40
EXPRESSION OF HYSYCE2 AND
HYTEXL2 IN
HYDRA VULGARIS AEP
43
INTERACTION OF HYSYCE2 AND HYTEXL2
EX VIVO
47
DISCUSSION 47
INDEX 4
9 CHAPTER III:
THE EVOLUTION
OF THE MAMMALIAN
LATERAL ELEMENT PROTEIN SYCP2 50
INTRODUCTION 50
RESULTS 51
PHYLOGENY OF SYCP2 51
SEQUENCE AND EXPRESSION
OF THE SYCP2 ORTHOLOGUE IN
HYDRA VULGARIS AEP
53
DISCUSSION 55
10 GENERAL DISCUSSION 57
10.1 THE DYNAMIC EVOLUTION
OF THE
SC 57
10.2 THE CNIDARIAN
SC: HYDRA
AS MODEL SYSTEM
FOR MEIOSIS RESEARCH? 60
11 PERSPECTIVES 67
12 MATERIAL 68
12.1 BIOLOGICAL MATERIAL 68
12.1.1
HYDRA
LINEAGE:
HYDRA VULGARIS AEP
68
12.1.2 BACTERIA 68
12.1.3 CELL LINES 69
12.1.4 ANTIBODIES 69
12.2 MOLECULAR MATERIAL 70
12.2.1 PLASMIDS 70
12.2.2 OLIGONUCLEOTIDES 71
12.2.3 RECOMBINANT DNA CONSTRUCTS 73
12.3 CHEMICALS 74
12.4 COMPUTER SOFTWARE AND
ONLINE TOOLS 75
12.5 EQUIPMENT 75
13 METHODS 77
13.1 MICROBIOLOGICAL METHODS 77
13.1.1 CULTURING OF BACTERIA 77
13.1.2 PRODUCTION OF COMPETENT BACTERIA 78
13.1.3 TRANSFORMATION OF
E. COLI
BACTERIA WITH PLASMID DNA 78
13.2 MOLECULAR METHODS 79
13.2.1 RNA EXTRACTION 79
13.2.2 PURIFICATION OF PLASMID DNA 79
13.2.3 POLYMERASE CHAIN REACTION (PCR) 79
13.2.4 DNA SEQUENCING 81
13.2.5 DNA GEL ELECTROPHORESIS 81
INDEX 5
13.2.6 PREPARATIVE DNA GEL
ELECTROPHORESIS AND GEL EXTRACTION 82
13.2.7 DNA CLONING 83
13.3 BIOCHEMICAL METHODS 84
13.3.1 PROTEIN GEL ELECTROPHORESIS 84
13.3.2 COOMASSIE STAINING 86
13.3.3 WESTERN BLOT ANALYSIS 86
13.3.4 EXPRESSION AND
PURIFICATION OF HIS-TAGGED FUSION
PROTEINS 88
13.3.5 CONCENTRATION AND PURIFICATION OF PROTEINS FROM AQUEOUS SOLUTIONS
90
13.3.6 SYNTHESIS OF SPECIFIC
ANTIBODIES 91
13.4 CELL CULTURE 94
13.4.1 CULTURING COS-7 CELLS 94
13.4.2 TRANSFECTION OF COS-7 CELLS 94
13.5 HISTOCHEMISTRY 95
13.5.1 HYBRIDIZATION HISTOCHEMISTRY: WHOLE MOUNT
IN SITU
HYBRIDIZATION 95
13.5.2 IMMUNOHISTOCHEMISTRY 98
13.5.3 IMMUNOCYTOCHEMISTRY 100
13.6 PHYLOGENETIC ANALYSIS 103
13.6.1 DATASET ASSEMBLY 103
13.6.2 MULTIPLE SEQUENCE ALIGNMENT 104
13.6.3 PHYLOGENETIC TREE CONSTRUCTION 105
14 REFERENCES 107
15 SUPPLEMENTS 115
15.1 SUPPLEMENTARY FIGURES 115
15.2 SUPPLEMENTARY TABLES 129
ERKLARUNG 146
LEBENSLAUF 147
LIST OF SCIENTIFIC PUBLICATIONS 148
PEER-REVIEWED ARTICLES 148
PUBLISHED ABSTRACTS 148
DANKSAGUNG 149
|
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| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2025-02-03T17:41:49Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027439869 |
| oclc_num | 885203983 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
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| physical | 149 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| record_format | marc |
| spellingShingle | Fraune, Johanna 1986- The evolutionary history of the mammalian synaptonemal complex Hydra Polyp (DE-588)4160852-5 gnd Maus (DE-588)4169148-9 gnd Evolution (DE-588)4071050-6 gnd Synaptinemal-Komplex (DE-588)4278711-7 gnd |
| subject_GND | (DE-588)4160852-5 (DE-588)4169148-9 (DE-588)4071050-6 (DE-588)4278711-7 (DE-588)4113937-9 |
| title | The evolutionary history of the mammalian synaptonemal complex |
| title_alt | Die Evolutionsgeschichte des Synaptonemalkomplexes der Maus |
| title_auth | The evolutionary history of the mammalian synaptonemal complex |
| title_exact_search | The evolutionary history of the mammalian synaptonemal complex |
| title_full | The evolutionary history of the mammalian synaptonemal complex von Johanna Fraune |
| title_fullStr | The evolutionary history of the mammalian synaptonemal complex von Johanna Fraune |
| title_full_unstemmed | The evolutionary history of the mammalian synaptonemal complex von Johanna Fraune |
| title_short | The evolutionary history of the mammalian synaptonemal complex |
| title_sort | the evolutionary history of the mammalian synaptonemal complex |
| topic | Hydra Polyp (DE-588)4160852-5 gnd Maus (DE-588)4169148-9 gnd Evolution (DE-588)4071050-6 gnd Synaptinemal-Komplex (DE-588)4278711-7 gnd |
| topic_facet | Hydra Polyp Maus Evolution Synaptinemal-Komplex Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-100043 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027439869&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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