Photoswitching proteins methods and protocols

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Weitere Verfasser: Cambridge, Sidney (HerausgeberIn)
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Sprache:English
Veröffentlicht: New York [u.a.] Humana Press 2014
Schriftenreihe:Methods in molecular biology 1148
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adam_text Titel: Photoswitching proteins Autor: Cambridge, Sidney Jahr: 2014 Contents Preface.......................................................... v Contributors.......................................................... ix 1 Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells........................................... 1 Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko 2 Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo............................................ 19 Sidney Cambridge 3 Optochemical Activation of Kinase Function in Live Cells................ 31 Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters 4 Photoswitching of Cell Surface Receptors Using Tethered Ligands......... 45 Andreas Reiner and Ehud Y. Isacoff 5 Photocontrol of AMPA Receptors with a Photochromic Ligand........... 69 Martin Sumser and Philipp Stawski 6 Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy........................................ 77 Nina Wittenmayer 7 Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins..... 89 Lauren R. Polstein and Charles A. Gersbach 8 Manipulation of Plasma Membrane Phosphoinositides Using Photoinduced Protein-Protein Interactions........................... 109 Olof Idevall-Hagren and Pietro DeCamilli 9 A Roadmap to Applying Cytogenetics in Neuroscience.................. 129 Consuelo Fois, Pierre-Hugues Prouvât, and Albrecht Stroh 10 Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors..... 149 Marion Mutter, Natalia Swietek, and Thomas A. Münch 11 Photoactivated Adenylyl Cyclases as Cytogenetic Modulators of Neuronal Activity............................................ 161 Wagner Steuer Costa, Jana Liewald, and Alexander Gottschalk 12 Structural Basis of Photoswitching in Fluorescent Proteins............... 177 Chenxi Duan, Virgile Adam, Martin Byrdin, and Dominique Bourgeois 13 Using Photoactivatable GFP to Track Axonal Transport Kinetics........... 203 Archan Ganguly and SubhojitRoy vii viii Contents 14 In Vivo Cell Tracking Using PhOTO Zebrafish........................ 217 William P. Dempsey, Hanyu Qin, and Periklis Pantazis 15 In Vivo Cytogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein............... 229 Cathleen Teh and Vladimir Korzh 16 Photoactivatable Fluorescent Proteins for Super-resolution Microscopy...... 239 Tuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus 17 pcSOFI as a Smart Label-Based Superresolution Microscopy Technique..... 261 Benjamien Moeyaert and Peter Dedecker Index............................................................... 277
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Springer protocols
spellingShingle Photoswitching proteins methods and protocols
Methods in molecular biology
title Photoswitching proteins methods and protocols
title_auth Photoswitching proteins methods and protocols
title_exact_search Photoswitching proteins methods and protocols
title_full Photoswitching proteins methods and protocols ed. by Sidney Cambridge
title_fullStr Photoswitching proteins methods and protocols ed. by Sidney Cambridge
title_full_unstemmed Photoswitching proteins methods and protocols ed. by Sidney Cambridge
title_short Photoswitching proteins
title_sort photoswitching proteins methods and protocols
title_sub methods and protocols
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027290958&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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