Lipid droplets
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| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2013
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in cell biology
116 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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MARC
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| 020 | |a 9780124080515 |9 978-0-12-408051-5 | ||
| 035 | |a (OCoLC)864741119 | ||
| 035 | |a (DE-599)BVBBV041457319 | ||
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| 245 | 1 | 0 | |a Lipid droplets |c ed. by Hongyuan Yang ... |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2013 | |
| 300 | |a XIII, 287 S., [8] S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 116 | |
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| 700 | 1 | |a Yang, Hongyuan |e Sonstige |4 oth | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-026903746 | |
Datensatz im Suchindex
| DE-BY-UBR_call_number | 88/WC 5100 |
|---|---|
| DE-BY-UBR_katkey | 5283878 |
| DE-BY-UBR_location | 88 |
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| _version_ | 1822757607439335424 |
| adam_text | Contents
Contributors
..............................................................................................................ix
Preface
...................................................................................................................xiii
CHAPTER
1
Proteomic Studies of Isolated Lipid Droplets
from Bacteria,
C. elegans,
and Mammals
.....................
ι
Huimin Na, Peng Zhang, Yunfeng Ding, Li Yang, Yang Wang,
Huina Zhang, Zhensheng Xie, Fuquan Yang, Simon Cichello,
Pingsheng
Liu
Introduction
..................................................................................2
1.1
Equipment
....................................................................................3
1.2
Reagents
.......................................................................................3
1.3
Methods
........................................................................................4
1.4
Discussion
..................................................................................10
Acknowledgment
.......................................................................12
References
..................................................................................12
CHAPTER
2
Analysis of Yeast Lipid Droplet Proteome and
Lipidome
..................................................................................
is
Claudia Schmidt,
Birgit Ploier,
Barbara
Koch, Günther Daum
Introduction
................................................................................16
2.1
Materials
.....................................................................................18
2.2
Methods
..............................
^
......................................................20
2.3
Results and Discussion
..............................................................26
2.4
Notes
................,............—:..........................................................33
Acknowledgments
.....................................................................33
References
..................................................................................34
CHAPTER
3
Visualization of Lipid Droplets in
C. elegans
by
Light and Electron Microscopy
........................................39
Ho Yi
Mak
Introduction
................................................................................40
3.1
Transgenic Expression of Lipid Droplet Markers
in
C. elegans
...............................................................................41
3.2
Visualization of Lipid Droplet Associated Proteins
by Laser Scanning Confocal Microscopy
..................................43
3.3
Visualization of Lipid Droplet Associated Proteins
by Spinning Disk Confocal Microscopy
...................................45
3.4
Visualization of Lipid Droplets by
ТЕМ
..................................46
3.5
Visualization of
Lipid
Droplet Associated Proteins
by Immunoelectron Microscopy
................................................47
Summary
....................................................................................48
Acknowledgments
.....................................................................48
References
..................................................................................48
CHAPTER
4
Analysis of
Lipid
Droplet Dynamics and
Functions in
Drosophila
melanogaster
.........................53
Han Lee, Ying Peng, Yi Guo
4.1
Genome-Wide
RNA
Interference Screens in
Drosophila
Cells to Identify Regulators for LD Biology
............................54
4.2
LD Dynamics in Different
Drosophila
Developmental Stages
................................................................59
4.3
LD Functions in
Drosophila
Physiology
...................................64
Acknowledgments
.....................................................................66
References
..................................................................................66
CHAPTER
5
Analysis of Oil Droplets in Microalgae
.........................71
Chengshi Yan, Jilian Fan, ChangchengXu
Introduction
................................................................................71
5.1
Nile Red as a Probe for Oil Droplets
........................................72
5.2
Confocal and Transmission Electron Microscopic
Observation
................................................................................76
5.3
Isolation and Purification of Oil Droplets
.................................79
Summary
....................................................................................79
Acknowledgments
.....................................................................79
References
..................................................................................80
CHAPTER
6
Studying Lipolysis in Adipocytes by Combining
siRNA Knockdown and Adenovirus-Mediated
Overexpression Approaches
.............................................83
Xiaodong Zhang, Bradlee L.
Heckmann,
Jun
Liu
Introduction and Rationale
.........................................................84
6.1
Materials and Reagents
..............................................................87
6.2
Methods
......................................................................................89
6.3
Concluding Remarks
..................................................................97
Acknowledgment
.....................................................................100
References
................................................................................100
CHAPTER
7
Analysis of
Lipid
Droplets in Hepatocytes
.................107
Huajin Wang, Ariel D. Quiroga, Richard Lehner
Introduction and Rationale
.......................................................108
7.1
Materials
...................................................................................
Ill
7.2
Methods
....................................................................................113
7.3
Discussion
................................................................................122
Conclusion
................................................................................125
Acknowledgments
...................................................................125
References
................................................................................126
CHAPTER
8
Analysis of Lipid Droplets in Cardiac Muscle
..........129
Hong Wang, Ming Lei, Ru-ching Hsia, Carole Sztalryd
Introduction and Rationale
.......................................................130
8.1
Isolation of Cardiac LD Proteins and Preparation of
Cardiac LDs for Analysis by Western Blot
............................132
8.2
Analysis of Cardiac LDs by 2D Analysis of LDs by
Conventional
ТЕМ
...................................................................137
8.3
Progress in
3D
EM Technology May Provide New Tools
to Study Cardiac LDs and Mitochondria Interactions
............142
Conclusions
..............................................................................145
Acknowledgments
...................................................................146
References
................................................................................146
CHAPTER
9
Imaging Cytoplasmic Lipid Droplets in
Enterocytes and Assessing Dietary
Fat Absorption
......................................................................
ш
Aki
Uchida, Hyeon Jeong Lee, Ji-Xin Cheng,
Kimberly K. Buhman
Introduction and Rationale
.......................................................152
9.1
Materials and Methods
.............................................................153
9.2
Discussion
................................................................................162
Acknowledgment
.....................................................................165
References
................................................................................165
CHAPTER
10
Lipid Droplets and Viral Infections
..............................167
Gregory Camus,
Dorothée A.
Vogt,
Andrew S. Kondratowicz,
Melanie Ott
Introduction
..............................................................................168
10.1
Materials
...................................................................................173
10.2
Methods
....................................................................................175
10.3
Discussion
................................................................................186
Acknowledgments
...................................................................187
References
................................................................................188
CHAPTER
11
Purification of Integral Membrane Proteins
and Lipid-Binding Assays
................................................191
David A. Gross, David L. Silver
Introduction and Rationale
.......................................................192
11.1
Materials
...................................................................................195
11.2
Methods
....................................................................................195
Conclusion and Applications
...................................................208
Acknowledgment
.....................................................................209
References
................................................................................209
CHAPTER
12
Imaging of Neutral Lipids and Neutral
Lipid Associated Proteins
................................................213
Lydia-Ann
L S.
Harris, James
R.
Skinner, Nathan E. Wolins
Introduction and Rationale
.......................................................214
12.1
Materials
...................................................................................214
12.2
Methods
....................................................................................215
12.3
Discussion
................................................................................221
Summary
..................................................................................224
Acknowledgments
...................................................................224
References
................................................................................225
CHAPTER
13
Imaging Lipid Droplets by Electron Microscopy
.....227
Toyoshi Fujimoto, Yuki Ohsaki, Michitaka Suzuki, Jinglei Cheng
Introduction
..............................................................................228
13.1
Probing the LD Core
................................................................231
13.2
Observation of the LD Surface
................................................235
13.3
Freeze-Substitution
...................................................................237
13.4
Immunoelectron Microscopy of LD-Associated Proteins
.......238
13.5
Freeze-Fracture Electron Microscopy
.....................................246
13.6
Future directions
......................................................................248
Acknowledgments
...................................................................248
References
................................................................................248
CHAPTER
14
Imaging Lipid Droplet Fusion and Growth
..................253
Zhiqi Sun, Jingyi Gong, Lizhen Wu, Peng Li
Introduction
..............................................................................254
14.1
Quantitative Measurement of LD Size
....................................256
14.2
Measuri ng Neutral Lipid Exchange Through FRAP
...............257
14.3
Measuring LD Fusion Rate
......................................................262
Conclusions
..............................................................................265
Acknowledgments
...................................................................265
References
................................................................................265
Index
.......................................................................................................................269
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV041457319 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T03:57:01Z |
| institution | BVB |
| isbn | 9780124080515 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026903746 |
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| physical | XIII, 287 S., [8] S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spellingShingle | Lipid droplets Methods in cell biology Cytologie (DE-588)4070177-3 gnd Methode (DE-588)4038971-6 gnd Sphärosom (DE-588)4182237-7 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4038971-6 (DE-588)4182237-7 (DE-588)4143413-4 |
| title | Lipid droplets |
| title_auth | Lipid droplets |
| title_exact_search | Lipid droplets |
| title_full | Lipid droplets ed. by Hongyuan Yang ... |
| title_fullStr | Lipid droplets ed. by Hongyuan Yang ... |
| title_full_unstemmed | Lipid droplets ed. by Hongyuan Yang ... |
| title_short | Lipid droplets |
| title_sort | lipid droplets |
| topic | Cytologie (DE-588)4070177-3 gnd Methode (DE-588)4038971-6 gnd Sphärosom (DE-588)4182237-7 gnd |
| topic_facet | Cytologie Methode Sphärosom Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026903746&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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