The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2013
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| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV041391460 | ||
| 003 | DE-604 | ||
| 005 | 20140110 | ||
| 007 | t| | ||
| 008 | 131031s2013 xx ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)862855417 | ||
| 035 | |a (DE-599)BVBBV041391460 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
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| 100 | 1 | |a Link, Jana |d 1986- |e Verfasser |0 (DE-588)1043591656 |4 aut | |
| 245 | 1 | 0 | |a The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression |c von Jana Link |
| 246 | 1 | 3 | |a Die Rolle meiotischer Kernhüllenkomponenten für Chromosomendynamiken und den meiotischen Ablauf |
| 264 | 1 | |c 2013 | |
| 300 | |a 113 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Würzburg, Univ., Diss., 2013 | ||
| 546 | |a Zsfassung in dt. und engl. Sprache | ||
| 650 | 0 | 7 | |a Kernhülle |0 (DE-588)4309987-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Meiose |0 (DE-588)4169347-4 |2 gnd |9 rswk-swf |
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| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:20-opus-83540 |
| 856 | 4 | 1 | |u https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-83540 |x Resolving-System |z kostenfrei |3 Volltext |
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| 912 | |a ebook | ||
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Datensatz im Suchindex
| DE-BY-TUM_katkey | 1959475 |
|---|---|
| _version_ | 1820806096122544128 |
| adam_text | SUMMARY 11
ZUSAMMENFASSUNG 12
1. INTRODUCTION 14
1.1 MEIOSIS - A SPECIAL TYPE OF CELL DIVISION
ESSENTIAL FOR GAMETOGENESIS 15
TELOMERE TETHERING & MOVEMENT - A CONSERVED FEATURE OF LEPTOTENE &
ZYGOTENE 16
SYNAPSIS FORMATION & RECOMBINATION - INTERDEPENDENT PROCESSES OF
ZYGOTENE & PACHYTENE
19
DEFECTS IN MEIOTIC PROGRESSION - CAUSES FOR INFERTILITY 22
1.2 THE NUCLEAR
LAMINA
- A VERSATILE COMPONENT OF THE
NUCLEAR
ENVELOPE 23
STRUCTURE & COMPOSITION OF THE LAMINA - AN ASSEMBLY MEDIATED BY
FUNCTIONAL DOMAINS OF THE
LAMIN PROTEINS 23
GENERAL FUNCTIONS OF THE LAMINA - ROLES BEYOND STRUCTURE & SCAFFOLD 25
THE LAMINA DURING MEIOSIS - UNIQUE PROTEINS & IMPLICATIONS FOR
SPECIALISED FUNCTIONS 28
1.3 THE
LINC COMPLEX
- THE LINKER
BETWEEN NUCLEOPLASM AND CYTOSKELETON 30
PROTEINS OF THE LINC COMPLEX - HIGHLY CONSERVED COMPONENTS FROM YEAST TO
MAN 31
FUNCTIONS OF LINC
COMPLEXES - A BROAD VARIETY OF ROLES MEDIATED BY SPECIALISED PROTEIN
COMBINATIONS 33
MEIOTIC LINC COMPLEXES
- THE PLAYERS THAT TETHER & MOVE MEIOTIC CHROMOSOMES 34
1.4 AIMS OF THIS THESIS - INVESTIGATING MEIOTIC TELOMERE ATTACHMENT AND
DYNAMICS FROM
DIFFERENT PERSPECTIVES 38
UNDERSTANDING THE ROLE OF THE MEIOTIC LAMINA - THE LAMIN C2 KNOCKOUT
MOUSE MODEL 38
INVESTIGATING THE MEIOTIC FUNCTIONS OF SUN2 - THE SUN1 KNOCKOUT MOUSE
MODEL 38
2. RESULTS 40
2.1
THE ROLE
OF THE
MEIOTIC NUCLEAR LAMINA IN CHROMOSOME DYNAMICS 40
BOUQUET STAGE FORMATION IS NOT ALTERED IN LAMIN C2 DEFICIENT
SPERMATOCYTES 42
BOUQUET STAGE RELEASE IS SIGNIFICANTLY DELAYED IN LAMIN C2 DEFICIENT
SPERMATOCYTES 43
DSB-REPAIR & RECOMBINATION ARE INCOMPLETE IN LAMIN C2 DEFICIENT MALES 45
2.2 THE
ROLE OF SUN2 IN MEIOTIC
TELOMERE ATTACHMENT
AND MOVEMENT 49
TELOMERE ATTACHMENT IS STILL PARTIALLY INTACT IN SUN1 DEFICIENT
SPERMATOCYTES 49
TELOMERES REMAIN STABLY ATTACHED THROUGHOUT PROPHASE I IN SUN1 DEFICIENT
SPERMATOCYTES
53
SUN2 AND KASH5 MEDIATE TELOMERE ATTACHMENT IN SUN1 DEFICIENT
SPERMATOCYTES 55
ATTACHED TELOMERES IN SUN1 DEFICIENT MICE ARE ABLE TO FORM BOUQUET-LIKE
CLUSTERS 58
HTTP://D-NB.INFO/1044800267
3. DISCUSSION 60
3.1
CONTRIBUTIONS OF NUCLEAR
ENVELOPE COMPONENTS TO MEIOTIC TELOMERE ATTACHMENT....
61
3.2 ROLES
OF THE
NUCLEAR
ENVELOPE IN MEIOTIC TELOMERE DYNAMICS 66
3.3 POSSIBLE
FUNCTIONS OF THE
FORMATION
AND RELEASE OF MEIOTIC
BOUQUET 73
4. FUTURE PROSPECTS 77
5. MATERIALS
AND METHODS 79
5.1 MATERIALS
USED 79
CHEMICALS 79
MICE STRAINS AND BREEDING 79
ANTIBODIES 79
OLIGONUCLEOTIDES 81
EQUIPMENT AND SOFTWARE 81
5.2 MOLECULAR
METHODS 83
GENOMIC DNA PREPARATION 83
MICE GENOTYPING BY PCR AMPLIFICATION OF GENOMIC DNA 83
DNA ELECTROPHORESIS 84
5.3 MICROSCOPIC METHODS 86
MOUSE TISSUE PREPARATION 86
PARAFFIN TISSUE EMBEDDING AND SECTIONING 86
FROZEN TISSUE EMBEDDING AND SECTIONING 87
TISSUE PREPARATION FOR ELECTRON MICROSCOPY 87
CHROMOSOME SPREADS 88
IMMUNOFLUORESCENCES ON PARAFFIN TISSUE SECTIONS 89
TELOMERE FLUORESCENT IN SITU HYBRIDISATION (TELOFISH) ON PARAFFIN
SECTIONS 90
IMMUNOFLUORESCENCE ON FROZEN SECTIONS 94
IMMUNOFLUORESCENCE ON CHROMOSOME SPREADS 95
5.4
IMAGE ACQUISITION
AND ANALYSIS 96
MICROSCOPY 96
3D RECONSTRUCTIONS 96
QUANTIFICATIONS OF TELOMERE ATTACHMENT 97
QUANTIFICATIONS OF BOUQUET FORMATIONS 97
STATISTICAL ANALYSIS 97
6. REFERENCES 99
7. ERKLARUNG 108
8. CURRICULUM VITAE 109
9. LEBENSLAUF. 110
10. PUBLICATIONS ILL
DANKSAGUNG 112
|
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| author | Link, Jana 1986- |
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| indexdate | 2024-12-24T03:55:30Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026839230 |
| oclc_num | 862855417 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
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| physical | 113 Bl. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| record_format | marc |
| spellingShingle | Link, Jana 1986- The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression Kernhülle (DE-588)4309987-7 gnd Meiose (DE-588)4169347-4 gnd |
| subject_GND | (DE-588)4309987-7 (DE-588)4169347-4 (DE-588)4113937-9 |
| title | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression |
| title_alt | Die Rolle meiotischer Kernhüllenkomponenten für Chromosomendynamiken und den meiotischen Ablauf |
| title_auth | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression |
| title_exact_search | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression |
| title_full | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression von Jana Link |
| title_fullStr | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression von Jana Link |
| title_full_unstemmed | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression von Jana Link |
| title_short | The role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression |
| title_sort | the role of meiotic nuclear envelope components in chromosome dynamics and meiotic progression |
| topic | Kernhülle (DE-588)4309987-7 gnd Meiose (DE-588)4169347-4 gnd |
| topic_facet | Kernhülle Meiose Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-83540 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026839230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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