Analysis of aortic arch development in zebrafish
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2013
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
TABLE O F CONTENTS
SUMMARY 1
C H A P T E R 1 INTRODUCTION 2
1.1 AORTIC ARCHES A N D CIRCUMJACENT TISSUE 2
1.1.1 FUNCTION O F THE AORTIC A R C H SYSTEM A N D ITS INVOLVEMENT IN
CONGENITAL
CARDIOVASCULAR ANOMALIES 2
1.1.2 PHARYNGEAL ARCHES 4
1.1.3 N C C S WITHIN THE PHARYNGEAL ARCHES 5
1.2 T H E MODEL ORGANISM ZEBRAFISH 6
1.2.1 ZEBRAFISH E M B R Y O S AS A TOOL TO S T U D Y VASCULAR D E V E L
O P M E N T IN GENERAL AND
AORTIC ARCH D E V E L O P M E N T IN PARTICULAR 6
1.2.2 AORTIC A R C H ( A A ) D E V E L O P M E N T IN ZEBRAFISH 7
1.3 T A T A BOX-BINDING PROTEIN-ASSOCIATED FACTOR 1 ( T A F 1 ) 8
1.3.1 TAF1 A N D ITS ROLE IN THE GENERAL TRANSCRIPTION M A C H I N E R Y
8
1.3.2 NON-CANONICAL FUNCTIONS O F TAF1 10
1.3.3 PHYSIOLOGICAL ROLE O F T A F I 10
1.4 A I M S O F THIS THESIS 11
C H A P T E R 2 MATERIAL A N D M E T H O D S 12
2.1 MATERIALS 12
2.1.1 FREQUENTLY USED BUFFERS A N D SOLUTIONS 12
2.1.2 ZEBRAFISH LINES 13
2.1.3 PLASMIDS 13
2.2 MOLECULAR BIOLOGICAL M E T H O D S 14
2.2.1 ISOLATION O F RIBONUCLEIC ACID ( R N A ) F R O M ZEBRAFISH E M B R
Y O S 14
2.2.2 C D N A SYNTHESIS 14
2.2.3 P C R TO A M P L I F Y D N A FRAGMENTS FOR C L O N I N G 15
2.2.4 ENDONUCLEASE RESTRICTION DIGESTS 16
2.2.5 A G A R O S E GEL ELECTROPHORESIS 16
2.2.6 EXTRACTION O F D N A F R O M AGAROSE GELS 16
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HTTP://D-NB.INFO/1035531747
IMAGE 2
2.2.7 LIGATION 17
2.2.8 TRANSFORMATION 17
2.2.9 C O L O N Y P C R S 18
2.2.10 ISOLATION O F NUCLEIC ACIDS U S I N G MINIPREP A N D MIDIPREP 19
2.2.11 SITE-DIRECTED MUTAGENESIS 19
2.2.12 CLONING O F CONSTRUCTS USED IN THIS THESIS 2 0
2.2.13 M R N A SYNTHESIS 21
2.2.14 G E N O T Y P I N G F O R T A F L MUTATION 2 2
2.2.15 S A M P L E PREPARATION F O R MICROARRAY G E N E EXPRESSION
ANALYSIS 2 4
2.2.16 P C R S USED FOR GENETIC M A P P I N G AND POSITIONAL CLONING 2 5
2.3 ZEBRAFISH MAINTENANCE 2 7
2.3.1 ZEBRAFISH HUSBANDRY A N D M A T I N G O F ADULTS 2 7
2.4 ZEBRAFISH EMBRYOLOGICAL M E T H O D S 2 7
2.4.1 MICROINJECTION 2 7
2.4.2 PHARMACOLOGICAL INHIBITOR TREATMENTS 2 8
2.4.3 W H O L E - M O U N T IN SITU HYBRIDIZATION 2 8
2.4.4 IMMUNOHISTOCHEMISTRY (ZN5 ANTIBODY STAINING) 3 2
2.4.5 T U N E L ASSAY 3 3
2.4.6 ANALYSIS O F CELL PROLIFERATION B Y 5-ETHYNYL-2 -DEOXYURIDINE ( E
D U )
INCORPORATION 3 4
2.4.7 CELL TRANSPLANTATIONS 3 5
2.5 I M A G I N G A N D CONFOCAL DATA PROCESSING 3 7
2.5.1 CONFOCAL M I C R O S C O P Y 3 7
2.5.2 T I M E - L A P S E IMAGING 3 7
2.5.3 CONFOCAL I M A G E PROCESSING A N D QUANTIFICATION 3 7
2.6 CELL CULTURE EXPERIMENTS 3 8
2.6.1 T H A W I N G CELLS 3 8
2.6.2 COUNTING CELL N U M B E R S A N D SPLITTING FOR TRANSFECTION 3 8
2.6.3 TRANSFECTION INTO C O S 7 CELLS 3 9
2.6.4 T A F L A N T I B O D Y STAINING ON C O S 7 CELLS 3 9
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IMAGE 3
C H A P T E R 3 RESULTS 41
3.1 DETAILED CHARACTERIZATION O F THE W A Y TO G O (WAY S4 9) AORTIC
ARCH ( A A ) PHENOTYPE 41
3.1.1 WAY S4(W M U T A N T E M B R Y O S FAIL TO G R O W A A 3 - 6 4 1
3.1.2 T H E M O R P H O L O G Y O F THE PHARYNGEAL ARCHES IS UNAFFECTED
IN WAY 409 M U T A N T
E M B R Y O S 4 2
3.1.3 T H E WAY 5,4 PHENOTYPE DIFFERS F R O M A A DEFECTS CAUSED B Y
T - B O X L ( T B X L )
DEPLETION 4 7
3.1.4 WAY S4NG M U T A N T E M B R Y O S D E V E L O P INITIAL A A CELL
CLUSTERS CONTAINING THE
CORRECT N U M B E R O F CCLLS BUT A A VESSELS FAIL TO FORM 4 8
3.1.5 R E D U C E D A A CCLL PROLIFERATION B U T NOT ELEVATED LEVELS O F
APOPTOSIS ARE
RESPONSIBLE F O R THE WAY 540 PHENOTYPE 5 2
3.2 A MUTATION IN THE ZEBRAFISH ORTHOLOGUE O F TAFL UNDERLIES THE WAY
5409 P H E N O T Y P E . . . 5 8
3.3 ANALYSIS O F THE FUNCTION O F T A F L IN A A D E V E L O P M E N T 6
3
3.3.1 ANALYSIS O F T A F L FUNCTIONAL D O M A I N S IN THE CONTEXT O F A
A DEVELOPMENT....63
3.3.2 SEARCH FOR D O W N S T R E A M TARGETS O F T A F L IN A A D E V E
L O P M E N T 6 8
3.3.3 PRESENCE O F T A F L IN N C C S IS REQUIRED F O R A A DEVELOPMENT
7 3
3.4 MODEL FOR FUNCTION O F T A F L IN A A D E V E L O P M E N T 7 8
C H A P T E R 4 DISCUSSION 7 9
4.1 T H E WAY S4(L9 M U T A T I O N AFFECTS THE A A VASCULATURE WITHOUT
CAUSING PHARYNGEAL A R C H
PATTERNING DCFECTS 7 9
4.2 A MUTATION IN THE ZEBRAFISH ORTHOLOGUE O F TAFL UNDERLIES THE WAY
S4OT P H E N O T Y P E . . . 8 0
4.3 T A F L PROMOTES A A CELL PROLIFERATION 81
4 . 4 ANALYSIS O F T A F L FUNCTIONAL D O M A I N S IN A A DEVELOPMENT 8
3
4.5 PRESENCE O F T A F L IN N C C S IS REQUIRED F O R A A D E V E L O P
M E N T 8 4
4 . 6 N C C S ARC REQUIRED FOR EARLY A A D E V E L O P M E N T 8 6
4.7 CONCLUDING R E M A R K S A N D FUTURE PERSPECTIVES 8 8
C H A P T E R 5 REFERENCES 9 0
- ILL -
IMAGE 4
C H A P T E R 6 A P P E N D I X 103
6.1 ABBREVIATIONS 103
6.2 DATA OBTAINED FROM C O U N T I N G O F A A CELLS A N D A A CELL
PROLIFERATION 108
6.3 SEQUENCES O F PRIMERS USED FOR CLONING A N D C D N A TESTING 109
6.4 SEQUENCES O F PRIMERS USED FOR GENERATION O F IN SITU HYBRIDIZATION
PROBES 1 10
6.5 SEQUENCES O F PRIMERS USED FOR SITE-DIRECTED MUTAGENESIS I L L
6.6 SEQUENCES O F PRIMERS USED FOR POSITIONAL CLONING 112
6.7 C U R R I C U L U M VITAE JANINE HILLMER 114
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| any_adam_object | 1 |
| author | Hillmer, Janine 1985- |
| author_GND | (DE-588)103553164X |
| author_facet | Hillmer, Janine 1985- |
| author_role | aut |
| author_sort | Hillmer, Janine 1985- |
| author_variant | j h jh |
| building | Verbundindex |
| bvnumber | BV041122512 |
| ctrlnum | (OCoLC)853280974 (DE-599)DNB1035531747 |
| dewey-full | 573.18538617482 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 573 - Specific physiological systems in animals |
| dewey-raw | 573.18538617482 |
| dewey-search | 573.18538617482 |
| dewey-sort | 3573.18538617482 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-12-24T03:24:59Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026098528 |
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| physical | IV, 116 S. Ill., graph. Darst. |
| publishDate | 2013 |
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| spellingShingle | Hillmer, Janine 1985- Analysis of aortic arch development in zebrafish Neuralleiste (DE-588)4503267-1 gnd Missbildung (DE-588)4039561-3 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Zebrabärbling (DE-588)4079468-4 gnd Aortenbogen (DE-588)4142762-2 gnd Kardiovaskuläres System (DE-588)4024665-6 gnd |
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| title | Analysis of aortic arch development in zebrafish |
| title_auth | Analysis of aortic arch development in zebrafish |
| title_exact_search | Analysis of aortic arch development in zebrafish |
| title_full | Analysis of aortic arch development in zebrafish vorgelegt von Janine Hillmer |
| title_fullStr | Analysis of aortic arch development in zebrafish vorgelegt von Janine Hillmer |
| title_full_unstemmed | Analysis of aortic arch development in zebrafish vorgelegt von Janine Hillmer |
| title_short | Analysis of aortic arch development in zebrafish |
| title_sort | analysis of aortic arch development in zebrafish |
| topic | Neuralleiste (DE-588)4503267-1 gnd Missbildung (DE-588)4039561-3 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Zebrabärbling (DE-588)4079468-4 gnd Aortenbogen (DE-588)4142762-2 gnd Kardiovaskuläres System (DE-588)4024665-6 gnd |
| topic_facet | Neuralleiste Missbildung Embryonalentwicklung Zebrabärbling Aortenbogen Kardiovaskuläres System Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026098528&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hillmerjanine analysisofaorticarchdevelopmentinzebrafish |