Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch & Buch Verl.
2013
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| Schriftenreihe: | Bilogical Science
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| Online-Zugang: | Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a Interaction of Helicobacter pylori with human myeloid antigenpresenting cells |b immunomodulating effects and their dependency on the bacterial cholesterol metabolism |c Michael Fehlings |
| 264 | 1 | |a Berlin |b Mensch & Buch Verl. |c 2013 | |
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Datensatz im Suchindex
| _version_ | 1804150462552735744 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
INTRODUCTION 1
1.1 THE HUMAN GASTRIC PATHOGEN, HELICOBACTER PYLORI 1
1.1.1 VIRULENCE FACTORS INVOLVED IN COLONIZATION OF H. PYLORI 2
1.2 MYELOID ANTIGEN-PRESENTING CELLS 5
1.2.1 MONOCYTES 7
1.2.2 MACROPHAGES 8
1.2.3 DENDRITIC CELLS 9
1.3 T-CELL IMMUNITY 11
1.4 THE IMMUNE RESPONSE AGAINST H. PYLORI AND BACTERIAL IMMUNE EVA
SION STRATEGIES 13
1.4.1 INNATE IMMUNE MECHANISMS AGAINST H. PYLORI 1.3
1.4.2 ADAPTIVE IMMUNE MECHANISMS AGAINST H. PYLORI 15
1.5 AIM OF THE STUDY 17
MATERIALS AND METHODS 19
2.1 MATERIALS 19
2.1.1 INSTRUMENTS 19
2.1.2 BUFFERS AND MEDIA 20
2.1.3 KITS 21
2.1.4 ANTIBODIES 22
2.1.5 BACTERIAL STRAINS 23
2.1.6 HUMAN BLOOD SAMPLES 23
2.2 METHODS 23
2.2.1 BACTERIA 23
2.2.1.1 CULTURE AND HARVESTING OF H. PYLORI 23
2.2.1.2 PREPARATION OF GLYCEROL STOCKS 24
2.2.2 HUMAN PRIMARY CELL PREPARATION 24
2.2.2.1 ISOLATION OF PERIPHERAL BLOOD MONONUCLEAR CELLS 2.
2.2.2.2 CELL COUNT 24
2.2.2.3 ISOLATION OF HUMAN MONOCYTES 25
2.2.2.4 GENERATION OF MONOCYTE-DERIVED DCS 25
2.2.2.5 GENERATION OF MI AND M2 MACROPHAGES 26
2.2.2.6 ISOLATION OF T CELLS 27
2.2.3 INFECTION AND EXPERIMENTAL ASSAYS 27
2.2.3.1 INFECTION OF HUMAN APCS WITH H. PYLORI 27
2.2.3.2 ANALYSIS OF SURFACE MOLECULES 28
2.2.3.3 PHAGOCYTOSIS EXPERIMENTS 28
2.2.3.4 MIXED LEUKOCYTE REACTION ASSAY 28
VII
HTTP://D-NB.INFO/1034518283
IMAGE 2
CONTENTS
2.2.3.5 ANALYSIS OF CYTOKINE SECRETION 2(J
2.2.4 DATA ANALYSIS AND STATISTICS 29
3 RESULTS 31
3.1 EFFECTS OF H. PYLORI INFECTION ON MONOCYTES 31
3.1.1 MONOCYTES RELEASE PREDOMINANTLY PRO-INFLAMMATORY CYTOKINES
AFTER H. PYLORI INFECTION 31
3.2 EFFECTS OF H. PYLORI INFECTION ON MI AND M2 MACROPHAGES 33
3.2.1 H. PYLORI INDUCES PHENOTYPIC ALTERATIONS IN MI AND M2 MACROPHAGES
33
3.2.2 H. PYLORI INFECTION PREVENTS ANTIGEN UPTAKE BY MI MACROPHAGES
40
3.2.3 H. PYLORI AFFECTS CD8 + T-CELL PROLIFERATION INDUCED BY MI
MACROPHAGES 41
3.2.4 MI AND M2 MACROPHAGES DIFFER REGARDING THEIR CYTOKINE
PROFILES UPON H. PYLORI INFECTION 43
3.3 EFFECTS OF H. PYLORI INFECTION ON DCS 47
3.3.1 H. PY/ORI-INFECTED DCS GET ACTIVATED, BUT DOWN-REGULATE DCSIGN
EXPRESSION 47
3.3.2 H. PYLORI INHIBITS SUBSEQUENT ANTIGEN UPTAKE OF DCS 51
3.3.3 H. PYLORI TRIGGERS T-CELL PROLIFERATION INDUCED BY DCS 52
3.3.4 H. PYLORI INFECTION INFLUENCES CYTOKINE SECRETION BY DCS 54
3.4 COMPARISON OF CYTOKINE SECRETION BY APCS AFTER H. PYLORI INFEC
TION 57
4 DISCUSSION 59
4.1 APCS REACT DIFFERENTLY UPON INTERACTION WITH H. PYLORI 59
4.2 THE H. PYLORI CHOLESTEROL METABOLISM MODULATES INDIVIDUAL APC
FUNCTIONS 64
4.3 CONCLUSIONS AND OUTLOOK 67
REFERENCES 6L)
|
| any_adam_object | 1 |
| author | Fehlings, Michael 1981- |
| author_GND | (DE-588)1035816768 |
| author_facet | Fehlings, Michael 1981- |
| author_role | aut |
| author_sort | Fehlings, Michael 1981- |
| author_variant | m f mf |
| building | Verbundindex |
| bvnumber | BV041087848 |
| classification_rvk | XD 4900 |
| ctrlnum | (OCoLC)861970979 (DE-599)DNB1034518283 |
| dewey-full | 616.33014 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.33014 |
| dewey-search | 616.33014 |
| dewey-sort | 3616.33014 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| id | DE-604.BV041087848 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:39:19Z |
| institution | BVB |
| isbn | 9783863872915 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026064510 |
| oclc_num | 861970979 |
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| physical | IX, 99 S. Ill., graph. Darst. |
| publishDate | 2013 |
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| publisher | Mensch & Buch Verl. |
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| series2 | Bilogical Science |
| spelling | Fehlings, Michael 1981- Verfasser (DE-588)1035816768 aut Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism Michael Fehlings Berlin Mensch & Buch Verl. 2013 IX, 99 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bilogical Science Zugl.: Berlin, Humboldt-Univ., Diss., 2013 Cholesterinstoffwechsel (DE-588)4147860-5 gnd rswk-swf Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd rswk-swf Antigenpräsentation (DE-588)4211468-8 gnd rswk-swf Immunevasion (DE-588)1032386185 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Helicobacter-pylori-Infektion (DE-588)4206296-2 s Immunevasion (DE-588)1032386185 s Antigenpräsentation (DE-588)4211468-8 s Cholesterinstoffwechsel (DE-588)4147860-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026064510&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fehlings, Michael 1981- Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism Cholesterinstoffwechsel (DE-588)4147860-5 gnd Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd Antigenpräsentation (DE-588)4211468-8 gnd Immunevasion (DE-588)1032386185 gnd |
| subject_GND | (DE-588)4147860-5 (DE-588)4206296-2 (DE-588)4211468-8 (DE-588)1032386185 (DE-588)4113937-9 |
| title | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism |
| title_auth | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism |
| title_exact_search | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism |
| title_full | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism Michael Fehlings |
| title_fullStr | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism Michael Fehlings |
| title_full_unstemmed | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism Michael Fehlings |
| title_short | Interaction of Helicobacter pylori with human myeloid antigenpresenting cells |
| title_sort | interaction of helicobacter pylori with human myeloid antigenpresenting cells immunomodulating effects and their dependency on the bacterial cholesterol metabolism |
| title_sub | immunomodulating effects and their dependency on the bacterial cholesterol metabolism |
| topic | Cholesterinstoffwechsel (DE-588)4147860-5 gnd Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd Antigenpräsentation (DE-588)4211468-8 gnd Immunevasion (DE-588)1032386185 gnd |
| topic_facet | Cholesterinstoffwechsel Helicobacter-pylori-Infektion Antigenpräsentation Immunevasion Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026064510&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT fehlingsmichael interactionofhelicobacterpyloriwithhumanmyeloidantigenpresentingcellsimmunomodulatingeffectsandtheirdependencyonthebacterialcholesterolmetabolism |