In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat
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| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
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2013
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| 008 | 130424s2013 xx ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)844081059 | ||
| 035 | |a (DE-599)BSZ381312259 | ||
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| 049 | |a DE-188 |a DE-355 |a DE-29T |a DE-83 | ||
| 082 | 0 | |a 573.873619352 |2 22//ger | |
| 100 | 1 | |a Schmitt, Arno Christian |e Verfasser |0 (DE-588)103388250X |4 aut | |
| 245 | 1 | 0 | |a In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |c presented by Arno Christian Schmitt |
| 264 | 1 | |c 2013 | |
| 300 | |a 79 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zusfassung in dt. Sprache. | ||
| 502 | |a Heidelberg, Univ., Diss., 2013 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025944648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-025944648 | |
Datensatz im Suchindex
| _version_ | 1819657859863937024 |
|---|---|
| adam_text | IMAGE 1
4
CONTENTS
ZUSAMMENF ASSUNG 1
SUMMARY 3
1. INTRODUCTION 6
1.1 CORTICAL COLUMNS AS REFERENCE FRAME TO DESCRIBE THE STRUCTURE O F
THE CORTEX 7
1.2 THE WHISKER STIMULUS REPRESENTATION I N THE SOMATOSENSORY CORTEX O F
THE RAT 7 1.2.1 INITIAL EXCITATORY INPUT TO THE NEURONS OF A BARREL
COLUMN 9
1.2.2 INPUT AND OUTPUT OF PYRAMIDAL NEURONS IN L2/3 10
1.2.3 INHIBITORY INPUT TO CORTICAL NEURONS 11
1.2.4 PUTATIVE CANDIDATES TO INHIBIT PNS IN J2J3 13
1.2.5 THE INHIBITORY NEURONS IN CORTICAL LAYER 1 13
1.3 APPROACH O F THE STUDY 14
2. METHODS 16
2.1 EXPERIMENTAL PROCEDURES 16
2.1.1 ANESTHESIA AND SURGERY 16
2.1.2 WHISKER STIMULATION 17
2.1.3 IMAGING OF INTRINSIC OPTICAL SIGNALS 17
2.1.4 TWO-PHOTON FLUORESCENCE MICROSCOPY 18
2.1.5 TARGETED IN VIVO WHOLE-CELL VOLTAGE RECORDINGS 19
2.1.6 PERFUSION, TISSUE PROCESSING AND STAINING PROCEDURE 20
2.1.7 RECONSTRUCTION OF MORPHOLOGIES 21
2.1.8 PHARMACOLOGICAL MANIPULATION OF LI 22
2.2 DATA ANALYSIS 22
2.2.1 DETERMINATION OF THE LAMINAR POSITION OF RECORDED NEURONS 23
2.2.2 DIFFERENTIATION OF INHIBITORY NEURONS AND EXCITATORY NEURONS 23
2.2.3 ANALYSIS OF THE AP FIRING 23
2.2.4 ANALYSIS OF THE SUBTHRESHOLD MEMBRANE POTENTIAL DYNAMICS 24
2.2.5 ANALYSIS OF MEMBRANE POTENTIAL VARIABILITY DURING GYKI INJECTION
24 22.6 ANALYSIS OF POPULATION ACTIVITY DURING GYKI INJECTION 25
12..7 STATISTICAL TESTS AND GRAPHICAL DATA PRESENTATION 25
3. RESULTS 26
3.1 NEURONS W I T H SOMA I N LI DISPLAY DENDRITE CHARACTERISTIC O F
INHIBITORY NEURONS 27
3.2 THE MEMBRANE POTENTIAL O F LI NEURONS AND U2/3 PNS PRIOR WHISKER
DEFLECTION 28
3.3 THE MORPHOLOGICAL STRUCTURE O F LI NEURONS 31
3.3.1 THE LENGTH OF THE DENDRITIC TREES OF LI NEURONS AND L^3 PNS 31
3.3.3 THE DISTRIBUTION OF AXONS AND DENDRITES OF LI NEURONS 32
3.4 THE ELECTROPHYSIOLOGICAL RESPONSES TO WHISKER DEFLECTIONS 37
3.4.1 THE MEAN SENSORY EVOKED AP FIRING WITHIN 100MS AND WITHIN 200MS
POST STIMULATION.. 37 3.4.2 THE TIME COURSE OF THE SENSORY RESPONSES 39
HTTP://D-NB.INFO/1036515214
IMAGE 2
5
3.4.3 THE ONSET OF EXCITATORY INPUT TO LI NEURONS OCCURS AS EARLY AS TO
2/3 PNS 42 3.4.4 THE EARLY AP FIRING WITHIN 25MS AND THE LATER AP
RESPONSE 42
3.4.5 THE VARIABILITY OF THE MEMBRANE POTENTIAL PRE AND DURING SENSORY
RESPONSES 44
3.5 CONSEQUENCES O F NEURONAL ACTIVITY I N LI FOR SENSORY RESPONSES O F
V2J3 P N S 47 3.5.1 EFFECT ON L^3 PNS IN SURROUNDING BARREL COLUMNS 49
3.5.2 THE AP FIRING OF V2/3 NEURONS IN THE PRINCIPAL WHISKER COLUMN
DURING PHARMACOLOGICAL MANIPULATION OF LI 53
4. DISCUSSION 55
4.1 IDENTIFICATION O F LI NEURONS 56
4.2 THE ONSET O F PSP INPUT TO LI NEURONS 57
4.4 THE MORPHOLOGY O F LI NEURONS 59
4.4.1 COMPARISON TO PREVIOUS DESCRIPTIONS OF LI NEURONS 59
4.4.2 POSSIBLE LIMITATIONS OF THE RECONSTRUCTIONS 60
4.4.3 SPATIAL EXTENT OF LI NEURON AXONS IN COMPARISON TO THE SPATIAL
EXTENT OF SENSORY EVOKED ACTIVITY IN THE CORTEX 61
4.5 LI MEDIATED REDUCTION O F MEMBRANE POTENTIAL VARIABILITY 62
4.6 A PROPOSED FUNCTION O F THE INHIBITORY NEURONS I N LI 65
ACKNOWLEDGMENT 68
REFERENCES 69
|
| any_adam_object | 1 |
| author | Schmitt, Arno Christian |
| author_GND | (DE-588)103388250X |
| author_facet | Schmitt, Arno Christian |
| author_role | aut |
| author_sort | Schmitt, Arno Christian |
| author_variant | a c s ac acs |
| building | Verbundindex |
| bvnumber | BV040966371 |
| ctrlnum | (OCoLC)844081059 (DE-599)BSZ381312259 |
| dewey-full | 573.873619352 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 573 - Specific physiological systems in animals |
| dewey-raw | 573.873619352 |
| dewey-search | 573.873619352 |
| dewey-sort | 3573.873619352 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-12-24T03:13:26Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025944648 |
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| physical | 79 Bl. Ill., graph. Darst. |
| publishDate | 2013 |
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| spellingShingle | Schmitt, Arno Christian In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |
| subject_GND | (DE-588)4113937-9 |
| title | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |
| title_auth | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |
| title_exact_search | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |
| title_full | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat presented by Arno Christian Schmitt |
| title_fullStr | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat presented by Arno Christian Schmitt |
| title_full_unstemmed | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat presented by Arno Christian Schmitt |
| title_short | In vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |
| title_sort | in vivo electrophysiology and morphological structure of neurons in layer 1 of the primary somatosensory cortex of the rat |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025944648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT schmittarnochristian invivoelectrophysiologyandmorphologicalstructureofneuronsinlayer1oftheprimarysomatosensorycortexoftherat |